Duverger V.,MerckSerono SA ZI B |
Sauvage C.,MerckSerono SA ZI B |
Kobr M.,MerckSerono SA ZI B |
Imhof M.O.,MerckSerono SA ZI B
Journal of Immunological Methods | Year: 2013
To ensure the selection of high producing recombinant cell lines, a number of screening processes were developed in the presence of detection agents. Here, CHO cell lines secreting recombinant antibodies were detected in semi-solid medium containing detection agents. The aim was to compare two protein A-derived detection agents to two commercial fluorescent antibodies directed against the Fc part of the antibody of interest: the protein A derived Z domain fused to the red fluorescent protein and protein A labelled with a fluorescent DylightTM 488 dye. All of these agents were compatible with cell recovery and colony formation, and specifically detected colonies secreting recombinant antibodies. Optimisation of the concentration of the fluorescent protein A allowed the identification of a higher number of good producers. Thus these data demonstrate that fluorescently labelled protein A-derivatives can be used for the selection of high producer cells. © 2013 Elsevier B.V.