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Priyadharsini R.,Indira Gandhi Medical College and Research Institute IGMCRI | Priyadharsini R.,Jawaharlal Institute of Postgraduate Medical Education & Research | Umamaheswaran G.,Jawaharlal Institute of Postgraduate Medical Education & Research | . Raja T.A.R.,Melmaruvathur Adhiparasakthi Institute of Medical science MAPIMS | And 7 more authors.
Journal of Community Genetics | Year: 2017

Several factors contribute to the development of coronary artery disease (CAD). Adenosine diphosphate (ADP) activated P2Y12 receptor also plays a key role in platelet activation and aggregation. It has been found that common variation in the P2Y12 gene was associated with increased platelet aggregation resulting in adverse cardiovascular outcomes. Thus, polymorphisms in the ADP receptor P2Y12 may contribute to the development of CAD. This study aims to determine the frequency distribution of platelet receptor polymorphism P2Y12 (i744T>C) in Tamilian population and to predict its possible role in CAD. Three hundred seventy-one subjects were recruited comprising of 221 healthy volunteers and 150 patients with CAD belonging to either sex, aged 18–60 years of Tamilian origin. Genomic DNA was extracted using phenol-chloroform method. Genotyping was done by PCR-RFLP (Polymerase chain reaction-restriction fragment length polymorphism). The C allele frequency of P2Y12 polymorphism in controls and cases was 8.4% and 17.7%, respectively. The TT, TC, and CC genotype frequencies in controls and cases were 83.7%, 15.8%, 0.5% and 66.7%, 31.3%, 2%, respectively. The genotype frequencies were in Hardy-Weinberg equilibrium. There was a significant association (p < 0.05) between the mutant genotypes of P2Y12(i744T>C) polymorphism and risk of CAD. The odds ratio was found to be 2.6. The variant allele frequency of P2Y12-i744T>C was significantly different from other populations. There was a significant association between the mutant genotypes of P2Y12(i744T>C) polymorphism and risk of developing CAD. Thus, the present study will emphasize on the relevance of pharmacogenetic testing of P2Y12(i744T>C) receptor gene polymorphism in CAD patients. © 2017 Springer-Verlag Berlin Heidelberg


Rao V.M.,Melmaruvathur Adhiparasakthi Institute of Medical science MAPIMS | Shanmugam L.,Melmaruvathur Adhiparasakthi Institute of Medical science MAPIMS | Surya R.,Melmaruvathur Adhiparasakthi Institute of Medical science MAPIMS | Kumar M.S.,Melmaruvathur Adhiparasakthi Institute of Medical science MAPIMS | And 6 more authors.
Biomedicine (India) | Year: 2014

Introduction: Inspiratory gasp produces vasoconstriction and decrease of blood flow in fingers. The response is highly variable and attempts to control variables have been unsuccessful in obtaining reproducible responses.Aim: The present study was conducted to study the pattern of vasoconstriction and decreased flow in the fingers.Materials and Methods: Peripheral blood flow was recorded using a photoelectric sensor.Results: The present study finds that the responses are different in each of the fingers studied in the degree of vasoconstriction, dispersion being wide as indicated by a wide SD. Recording from two adjacent fingers showed that the odds of obtaining different responses were significantly high. Serial recording at 20s interval thrice showed that three successive responses were likely to be the same. However sometimes a progressive increase or a progressive decrease could be seen. There was no significant difference in response between the dominant and non dominant sides though mean indicated that there was 14% greater vasoconstriction on the right side.Conclusion: It is concluded that difference in degree of activation of sympathetic in different regions, recruitment and adaptation of sympathetic activity are the basis of variability of response in the inspiratory gasp reflex.

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