Tsai C.-W.,National Central University |
Ruaan R.-C.,National Central University |
Liu C.-I.,Meiho University
Langmuir | Year: 2012
The hydrophobic interaction between antimicrobial peptides and membrane hydrophobic cores is usually related to their cytotoxicity. In this study, the adsorption mechanism of five plasma membrane-associated peptides, indolicidin (IL) and its four derivatives, with hydrophobic ligands was investigated to understand the relationship between peptide hydrophobicity and bioactivity. The hydrophobic adsorption mechanisms of IL and its derivatives were interpreted thermodynamically and kinetically by reversed-phase chromatography (RPC) analysis and surface plasmon resonance (SPR) measurement, respectively. IL and its derivatives possess a similar random coil structure in both aqueous and organic solvents. Thermodynamic analysis showed that the binding enthalpy of peptides with higher electropositivity was lower than those with lower electropositivity and exhibited unfavorable binding entropy. Higher electropositivity peptides adsorbed to the hydrophobic surface arising from the less bound solvent on the peptide surface. A comparison with the kinetic analysis showed that IL and its derivatives adopt a two-state binding model (i.e., adsorption onto and self-association on the hydrophobic acyl chain) to associate with the hydrophobic surface, and the binding affinity of peptide self-association correlates well with peptide hemolysis. Consequently, this study provided a novel concept for understanding the action of plasma membrane-associated peptides. © 2012 American Chemical Society.
Kuo S.-Y.,University of Macau |
Cuvelier S.J.,Sam Houston State University |
Huang Y.-S.,Meiho University
International Journal of Offender Therapy and Comparative Criminology | Year: 2014
This study identified risk factors for prison victimization in Taiwan with an application of Western literature and assessed the extent of its applicability in an Eastern context. The sample was drawn from four male prisons located in Northern, Central, Southern, and Eastern Taiwan; a total of 1,181 valid surveys were collected. The results generally support the major findings of the extant Western studies. Crowding, however, was not significantly associated with the risk of victimization in any of the statistical models, which might be related to the different experiences and living conditions in the free community between Taiwanese and American inmates. This study generated clear policy implications, which may reduce prison victimization and engender a greater sense of well-being in the prison environment. © The Author(s) 2012.
Lin C.-C.,Meiho University |
Liu C.-F.,National Taipei University of Nursing and Health Sciences
Climacteric | Year: 2015
Objective To perform a meta-analysis examining the efficacy of phytoestrogens for the relief of menopausal symptoms. Methods Medline, Cochrane, EMBASE, and Google Scholar databases were searched until September 30, 2013 using the following key words: vasomotor symptoms, menopausal symptoms, phytoestrogens, isoflavones, coumestrol, soy, red clover. Inclusion criteria were (1) randomized controlled trial (RCT), (2) perimenopausal or postmenopausal women experiencing menopausal symptoms, (3) intervention with an oral phytoestrogen. Outcome measures included Kupperman index (KI) changes, daily hot flush frequency, and the likelihood of side-effects. Results Of 543 potentially relevant studies identified, 15 RCTs meeting the inclusion criteria were included. The mean age of the subjects ranged from 49 to 58.3 and 48 to 60.1 years, respectively, in the placebo and phytoestrogen groups. The number of participants ranged from 30 to 252, and the intervention periods ranged from 3 to 12 months. Meta-analysis of the seven studies that reported KI data indicated no significant treatment effect of phytoestrogen as compared to placebo (pooled mean difference = 6.44, p = 0.110). Meta-analysis of the ten studies that reported hot flush data indicated that phytoestrogens result in a significantly greater reduction in hot flush frequency compared to placebo (pooled mean difference = 0.89, p < 0.005). Meta-analysis of the five studies that reported side-effect data showed no significant difference between the two groups (p = 0.175). Conclusion Phytoestrogens appear to reduce the frequency of hot flushes in menopausal women, without serious side-effects. © 2014 International Menopause Society.
Wu W.-H.,Kaohsiung Medical University |
Chiou W.-B.,Sun Yat Sen University |
Kao H.-Y.,Kaohsiung Medical University |
Alex Hu C.-H.,Meiho University |
Huang S.-H.,GigaMedia Ltd
Computers and Education | Year: 2012
Previous literature reviews or meta-analysis based studies on game-assisted learning have provided important results, but few studies have considered the importance of learning theory, and coverage of papers after 2007 is scant. This study presents a systematic review of the literature using a meta-analysis approach to provide a more comprehensive analysis and synthesis of relevant studies based on four orientations of learning theories and principles: behaviorism, cognitivism, humanism, and constructivism. Major findings of this study include that the majority of published studies were not based on learning theory and the development of learning theory orientations has prompted more studies to focus on constructivism and humanism than on behaviorism and cognitivism. In addition, most studies adopted a descriptive approach, followed by experimental methods and surveys, and most presented positive outcomes. These findings not only advance understanding of game-assisted learning from the important perspective of learning theory, but also provide useful insights for researchers and educators in issues related to game-assisted learning. © 2012 Elsevier Ltd. All rights reserved.
Wu W.-H.,National Kaohsiung University of Applied Sciences |
Wu W.-H.,Kaohsiung Medical University |
Jim Wu Y.-C.,National Sun Yat - sen University |
Chen C.-Y.,Meiho University |
And 3 more authors.
Computers and Education | Year: 2012
Two previous literature review-based studies have provided important insights into mobile learning, but the issue still needs to be examined from other directions such as the distribution of research purposes. This study takes a meta-analysis approach to systematically reviewing the literature, thus providing a more comprehensive analysis and synthesis of 164 studies from 2003 to 2010. Major findings include that most studies of mobile learning focus on effectiveness, followed by mobile learning system design.; surveys and experiments were used as the primary research methods. Also, mobile phones and PDAs are currently the most widely used devices for mobile learning but these may be displaced by emerging technologies. In addition, the most highly-cited articles are found to focus on mobile learning system design, followed by system effectiveness. These findings may provide insights for researchers and educators into research trends in mobile learning. © 2012 Elsevier Ltd. All rights reserved.
Yang P.,Kaohsiung Medical University |
Chang C.-L.,Kaohsiung Medical University |
Chang C.-L.,Kaohsiung Armed Forces General Hospital |
Chang C.-L.,Meiho University
Current Pharmaceutical Design | Year: 2014
Autism spectrum disorders (ASD) are developmental disorders that are characterized by deficits in reciprocal social interactions and communication, as well as by the presence of impairing repetitive behaviors and restricted interests. Prior work examining human pathology, and model systems and genetic studies have all led to the current conceptualization of ASD as a disorder of synaptic formation and functioning (a “synapsopathy”). In this regard, glutamate, the major excitatory neurotransmitter in central nervous system synaptic transmission, with roles in learning, memory and synaptic plasticity, is hypothesized to play an important role in the pathophysiology of ASD. Molecules targeting glutamate signaling have been suggested to possess therapeutic potential for ASD treatment. This review focuses on the role of the structure and function of glutamate receptors, describes synaptic cell-adhesion molecule pathways related to glutamate and/or ASD, introduces a rare disease approach in the development of novel drugs for ASD treatment, and reports on glutamate-related clinical trials. We will also present promising new techniques using human-induced pluripotent stem cells, which may afford researchers the ability to study the relationships between clinical phenotypes, cellular responses and glutamate involvement in ASD. © 2014 Bentham Science Publishers.
Chiu A.-F.,Tzu Chi University |
Huang M.-H.,Tzu Chi University |
Wang C.-C.,Meiho University |
Kuo H.-C.,Chung Yuan Christian University
International Journal of Urology | Year: 2012
Objectives: To assess overactive bladder and its component symptoms among patients with type2 diabetes mellitus and to explore whether higher glycosylated hemoglobin and other factors increase the risk of overactive bladder symptoms. Methods: A total of 279 diabetes mellitus patients from our outpatient clinic, and 578 age- and sex-matched subjects without diabetes mellitus from public health centers were enrolled from May to September of 2010. The collected data included overactive bladder and its component symptoms measured by using the Overactive Bladder Symptom Score, and collecting demographic and clinical data. Overactive bladder was defined as total Overactive Bladder Symptom Score ≥3 and urgency score ≥2 (once a week or more). Results: Diabetes mellitus patients had a significantly higher proportion of overactive bladder symptoms/urgency compared with the controls (28.0% vs 16.3%, odds ratio 2.03, 95% confidence interval 1.44-2.86), as well as nocturia (48.0% vs 39.1%, odds ratio 1.44, 95% confidence interval 1.08-1.93). There were no significant effects of diabetes mellitus on urge urinary incontinence (14.0% vs 10.9%, odds ratio 1.32, 95% confidence interval 0.86-2.04) and daytime frequency (26.9% vs 32.4%, odds ratio 0.77, 95% confidence interval 0.56-1.05). After adjusting for all variables, high glycosylated hemoglobin levels were significantly associated with overactive bladder/urgency (odds ratio 1.24, 95% confidence interval 1.06-1.45), urge urinary incontinence (odds ratio 1.20, 95% confidence interval 1.00-1.45) and nocturia (odds ratio 1.17, 95% confidence interval 1.01-1.35). Conclusions: Patients with type2 mellitus present more overactive bladder symptoms/urgency and nocturia than controls. Among diabetic patients, higher glycosylated hemoglobin level represents an independent predictor of overactive bladder /urgency, urge urinary incontinence and nocturia. © 2012 The Japanese Urological Association.
Chen J.-T.,Taiwan Agricultural Research Institute |
Su H.-J.,Meiho University |
Huang J.-W.,National Chung Hsing University
Journal of Agricultural and Food Chemistry | Year: 2012
Secondary metabolites of Clitocybe nuda displayed antimicrobial activity against Phytophthora capsici. The culture filtrate of C. nuda was extracted with ethanol and chromatographically separated on a Sephadex LH-20 column and fractionated on a silica gel column to give eight fractions. These fractions were tested for the ability to inhibit zoospore germination of P. capsici. The most active fraction was further purified by silica gel column chromatography to yield three compounds: 2-methoxy-5-methyl-6-methoxymethyl-p-benzoquinone (1), 6-hydroxy-2H-pyran-3-carbaldehyde (2), and indole-3-carbaldehyde (3), all new to C. nuda. At a concentration of 500 mg/L, compound 3 showed complete inhibition of zoospore germination, while compounds 1 and 2 showed inhibition rates of 97 and 86%, respectively. To our knowledge, compound 1 is a newly discovered compound and, for the other two compounds, this is the first report in C. nuda. These compounds are potential candidates for new edible fungi-derived pesticides for the control of plant diseases. © 2012 American Chemical Society.
Juang D.F.,Meiho University |
Lee C.H.,Meiho University |
Hsueh S.C.,Meiho University
Bioresource Technology | Year: 2012
Electricity generation capabilities of microbial fuel cell with different light power on algae grown cathode were compared. Results showed that microbial fuel cell with 6 and 12. W power of light always produced higher voltage and power density than with 18 and 26. W. Similarly, microbial fuel cell with 6 and 12. W of light power always displayed higher Coulombic efficiency and specific power than the one with 18 and 26. W. The results also showed that microbial fuel cell with covered anodic chamber always displayed higher voltage, power density, Coulombic efficiency and specific power than the one without covered anodic chamber. Binary quadratic equations can be used to express the relationships between the light power and the voltage, power density, Coulombic efficiency and specific power. Although lower power of light on algae grown cathode and covering anodic chamber will increase system's electricity production, they will not significantly reduce its internal resistance. © 2012 Elsevier Ltd.
Lee M.-H.,MeiHo University
Journal of Nursing | Year: 2016
Clinical nursing practice uses professional nursing techniques, empathy, and moral judgment to help relieve the pain of patients who are suffering physically or mentally from trauma or illness. Thus, nursing involves sacred tasks. According to Confucian reasoning, morality is expressed through everyday actions. Providing care to patients arouses concern and sympathy in the caregiver, which induces him or her to fully apply professional knowledge to this task. This conduct demonstrates the moral conscience of nurses. The Chinese adage "Tian-tao hsing-ming hsiang kuan tung" (the way of heaven and life are correlated), attributed to Northern Song dynasty scholar Zhang Tsai, aptly explains the sacred mission of the nursing profession. Zhang held that all humans are born with a conscience that is given by heaven. However, human senses and desires may suppress this conscience. As Zhang Tsai suggested, the conscience must be cultivated in order to be manifested. The increase in recent years of international academic discussion/research related to the value and meaning of nursing and of exploration into how to promote the moral competence of nurses, not only provide support to Zhang's argument that "tian-tao" and "hsing-ming" are correlated but also provide positive momentum toward enhancing the self-worth of nurses and enabling patients to receive better care.