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Pedersen H.L.,Copenhagen University | Fangel J.U.,Copenhagen University | McCleary B.,Megazyme International Ireland Ltd. | Ruzanski C.,John Innes Center | And 11 more authors.
Journal of Biological Chemistry | Year: 2012

Microarrays are powerful tools for high throughput analysis, and hundreds or thousands of molecular interactions can be assessed simultaneously using very small amounts of analytes. Nucleotide microarrays are well established in plant research, but carbohydrate microarrays are much less established, and one reason for this is a lack of suitable glycans with which to populate arrays. Polysaccharide microarrays are relatively easy to produce because of the ease of immobilizing large polymers noncovalently onto a variety of microarray surfaces, but they lack analytical resolution because polysaccharides often contain multiple distinct carbohydrate substructures. Microarrays of defined oligosaccharides potentially overcome this problem but are harder to produce because oligosaccharides usually require coupling prior to immobilization. We have assembled a library of well characterized plant oligosaccharides produced either by partial hydrolysis from polysaccharides or by de novo chemical synthesis. Once coupled to protein, these neoglycoconjugates are versatile reagents that can be printed as microarrays onto a variety of slide types and membranes. We show that these microarrays are suitable for the high throughput characterization of the recognition capabilities of monoclonal antibodies, carbohydrate-binding modules, and other oligosaccharide-binding proteins of biological significance and also that they have potential for the characterization of carbohydrate-active enzymes. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.


Trademark
Megazyme International Ireland Ltd. | Date: 2000-12-19

DIAGNOSTIC REAGENTS OR TESTING REAGENTS FOR SCIENTIFIC OR RESEARCH USE; KITS CONTAINING DIAGNOSTIC REAGENTS OR TESTING REAGENTS FOR SCIENTIFIC OR RESEARCH USE.


Trademark
Megazyme International Ireland Ltd. | Date: 2000-09-12

DIAGNOSTIC REAGENTS OR TESTING REAGENTS FOR SCIENTIFIC OR RESEARCH USE; KITS CONTAINING DIAGNOSTIC REAGENTS OR TESTING REAGENTS FOR SCIENTIFIC OR RESEARCH USE.


Trademark
Megazyme International Ireland Ltd | Date: 2005-05-31

Diagnostic preparations for scientific or industrial use; diagnostic test kits comprised of enzymes, substrates and reagents for scientific, research and industrial use to check the activity of enzymes and concentration of carbohydrates and for other scientific and research use; diagnostic kits comprised of enzymes, substrates and reagents for scientific, research and industrial use; enzymes, substrates and reagents for scientific or research use.

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