Singh G.,Insect Resistance Group |
Sachdev B.,Insect Resistance Group |
Sharma N.,Meerut College |
Seth R.,University of Delhi |
Bhatnagar R.K.,Insect Resistance Group
Applied and Environmental Microbiology | Year: 2010
Vegetative insecticidal protein (Vip3A) is synthesized as an extracellular insecticidal toxin by certain strains of Bacillus thuringiensis. Vip3A is active against several lepidopteran pests of crops. Polyphagous pest, Spodoptera frugiperda, and its cell line Sf21 are sensitive for lyses to Vip3A. Screening of cDNA library prepared from Sf21 cells through yeast two-hybrid system with Vip3A as bait identified ribosomal protein S2 as a toxicity-mediating interacting partner protein. The Vip3A-ribosomal-S2 protein interaction was validated by in vitro pulldown assays and by RNA interference-induced knockdown experiments. Knockdown of expression of S2 protein in Sf21 cells resulted in reduced toxicity of the Vip3A protein. These observations were further extended to adult fifth-instar larvae of Spodoptera litura. Knockdown of S2 expression by injecting corresponding double-stranded RNA resulted in reduced mortality of larvae to Vip3A toxin. Intracellular visualization of S2 protein and Vip3A through confocal microscopy revealed their interaction and localization in cytoplasm and surface of Sf21 cells. Copyright © 2010, American Society for Microbiology.
Saxena D.,Meerut College
Indian Journal of Physics | Year: 2010
The effect of total solar eclipse on atmospheric conductivities has been studied. Continuous measurements of both types of positive and negative electrical conductivities and some meteorological parameters in conjunction with the total solar eclipse of 22 July, 2009 were made from 21 to 23 July, 2009 at Roorkee (29°52′ N, 77°53′ E, 275 m above sea level). Roorkee observed 90% maximum obscurity of eclipse since it was close to the path of totality. The event started from 05:28 AM IST and ended at 07:42 AM IST with the total eclipse at 06:30 AM IST Considerable increase of both positive and negative conductivities were recorded during the eclipse with respect to those made on any other day. Increase in relative humidity and decrease in temperature were also observed during the period of eclipse. © 2010 IACS.
Singh J.,Meerut College |
Singh P.,University of Lucknow
Bioinorganic Chemistry and Applications | Year: 2012
N-substituted pyridine hydrazide (pyridine-2-carbonyl chloride and 4-chloro-benzoic acid hydrazide) undergoes hydrazide formation of the iminic carbon nitrogen double bond through its reaction with cobalt(II), nickel(II), and copper(II) metal salts in ethanol which are reported and characterized based on elemental analyses, IR, solid reflectance, magnetic moment, molar conductance, and thermal analysis (TG). From the elemental analyses data, 1: 2 metal complexes are formed having the general formulae [MCl 2(HL) 2] · yH 2O (where M = Co(II), Ni(II), and Cu(II), y = 13). The important infrared (IR) spectral bands corresponding to the active groups in the ligand and the solid complexes under investigation were studied. IR spectra show that ligand is coordinated to the metal ions in a neutral bidentate manner with ON donor sites. The solid complexes have been synthesized and studied by thermogravimetric analysis. All the metal chelates are found to be nonelectrolytes. From the magnetic and solid reflectance spectra, the complexes (cobalt(II), nickel(II), and copper(II)) have octahedral and square planner geometry, respectively. The antibacterial and antifungal activity's data show that the metal complexes have a promising biological activity comparable with the parent ligand against bacterial and fungal species. © 2012 Jagvir Singh and Prashant Singh.
Singh D.,Meerut College
Biochemical and Cellular Archives | Year: 2015
Newly hatched W.L.H. chicks were infected with Ascaridia galli eggs low dose (500 eggs) and subsequently treated with cadmium acetate (5mg/Kg body wt.). After 30 days of post infection and treatment spleen showed various pathological changes. With low dose of infection the capsular was ruptured at various places and white pulp showed inflammatory edema, presence of secondary nodules, separation of inner capsular wall with inflammatory and non inflammatory edema. Sinus venosus showed lymphoid hyperplasia.
Singh L.,Meerut College |
Biochemical and Cellular Archives | Year: 2012
As to the current status of functional foods and beverages in India, we are amongst top 10 importers of the world and in exports lag behind China. Hence, Nutraceutical and Nutrigenomic research in India needs to be boosted as we have big opportunities in the field that exceeds organic sector due to the existence of rich biodiversity in the region. Due to their physiological advantage, health benefits, and additive effects on drug therapy, functional foods, beverages, supplements, and biofortifications are emerging as important branch of food science with multidisciplinary approach. An updated overview of Prebiotics, Probiotics, Synbiotics, Fermented foods, Macrobiotics, Nutraceuticals, Pharmaceuticals, Nutrigenomics and Biopharming and their health effects have been discussed herein therefore, along with increasing Indian awareness, contribution, options and priorities.