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Singh R.G.,Meenakshi Ammal Dental College and Hospital
The International journal of oral & maxillofacial implants | Year: 2011

To evaluate the osteogenic potential of commercially pure titanium after different surface treatments. Thirty commercially pure grade 2 titanium disks of similar designs and dimensions were divided into three groups. In the first group (group C), polished samples were used as the control group. In the second group (group SG), an oxide layer was coated on the samples using a sol-gel dip coating technique. In the third group (group SA), samples were sandblasted and treated with different acids in succession to etch the samples. Scanning electron microscopy with energy-dispersive x-ray spectroscopy of one sample from each group was performed to observe the surface morphology and surface composition. The surface roughness of the samples was evaluated with a surface profilometer. To evaluate the effect of the SA and SG treatments on the samples' osteogenic potential, samples from each group underwent cell culture study using human osteosarcoma osteoblast cell lines. Scanning electron microscopy of one sample from each group was performed to observe cell morphology and attachment. The SA and SG surfaces were rougher than that of the control group. There was a significant increase in oxygen content in groups SG and SA. Cell sheets were able to penetrate into the pores and adhered inside the valleys of the SA samples, suggesting excellent attachment. Source

Rajasekaran R.,Anna University | Aruna P.R.,Anna University | Koteeswaran D.,Meenakshi Ammal Dental College and Hospital | Bharanidharan G.,Anna University | And 2 more authors.
Journal of Biomedical Optics | Year: 2014

Steady-state and time-resolved fluorescence spectroscopy were employed in the discrimination of cervical cancer patients from healthy subjects using urine samples. Fluorescence emission at 390 and 440 nm was considered to monitor the fluorescence of indoxyl sulfate and neopterin. Significant spectral differences were observed between healthy and cancer subjects. Different ratio parameters were calculated from the spectral intensity at 280- and 350-nm excitation and were subjected to stepwise linear discriminant analysis. In total, 84.0% of samples were correctly classified at 280 nm and 96.4% were correctly classified at 350 nm. The fluorescence decay kinetics of urine samples at 390-nm emission was best described by biexponential fits, whereas the decay characteristics at 440 nm of urine samples was best explained by bi-exponential fits and, in some cases, by tri-exponential fits. However, the decay kinetics of both indoxyl sulfate and neopterin standards was well described by bi-exponential decays. Based on the fluorescence emission characteristics and statistical analysis, the fluorophores indoxyl sulfate, neopterin, and riboflavin may be considered as potential biomarkers for cervical cancer diagnosis. © 2014 Society of Photo-Optical Instrumentation Engineers (SPIE). Source

Rajasekaran R.,Anna University | Aruna P.,Anna University | Koteeswaran D.,Meenakshi Ammal Dental College and Hospital | Baludavid M.,Government Arignar Anna Memorial Cancer Hospital | Ganesan S.,Anna University
Journal of Fluorescence | Year: 2014

Urine is one of the diagnostically potential bio fluids, as it contains many metabolites and some of them are native fluorophores. These fluorophores distribution and the physiochemical properties may vary during any metabolic change or at different pathologic conditions. Since urine is a multicomponent fluid, synchronous luminescence technique, a powerful tool has been adopted to analyse multicomponents in single spectrum and to resolve emission spectrum without much of photobleaching of fluorophores. In this study, urine samples of both normal subjects and cancer patients were characterised using synchronous luminescence spectroscopy with a Stokes shift of 20 nm. Different ratio parameters were calculated from the intensity values of the synchronous luminescence spectra and they were used as input variables for a multiple linear discriminant analysis across normal and cancer groups. The stepwise linear discriminant analysis classifies 90.3 % of the original grouped cases and 88.6 % of the cross-validated grouped cases correctly. © 2014 Springer Science+Business Media New York. Source

Sreekumar K.,Meenakshi Ammal Dental College and Hospital | Bhargava D.,Meenakshi Ammal Dental College and Hospital
Oral and Maxillofacial Surgery | Year: 2011

Purpose: This randomized double-blind investigation was conducted to compare the onset and duration of action of soft tissue and pulpal anesthesia with three volumes of 4% articaine with 1:100,000 epinephrine in maxillary infiltration anesthesia. The injection discomfort associated with three volumes of infiltration anesthesia was also assessed. Materials and methods: A total of 10 subjects received 0.6 mL (group 1), 0.9 mL (group 2), and 1.2 mL (group 3) of the anesthetic buccal to the upper canine. Test teeth were assessed with electrical stimulation to determine onset and duration of pulpal anesthesia. Soft tissue anesthesia was assessed by pin-prick test, and injection discomfort was assessed using a visual analogue scale. The statistical analysis of the data recorded was carried out with one-way ANOVA and post-hoc tests. Results: The 1.2-mL dose induced faster onset of pulpal anesthesia, a higher success rate, and a longer duration of soft tissue/pulpal anesthesia than was achieved with 0.6 mL (P < 0.005). No differences in injection discomfort were observed between treatment groups. Group 3 where 1.2 mL of local anesthetic was injected showed faster onset and longer duration of action of articaine. Group 3 also had longer soft tissue anesthesia as compared to groups 1 and 2. Conclusion: Maxillary infiltration anesthesia with articaine and epinephrine has a faster onset, a greater success rate, and a longer duration when a volume of 1.2 mL is used than when volumes less than 1.0 mL are used. Palatal tissues were anesthetized with the highest concentration (1.2 mL) in our study (30% of cases). © 2011 Springer-Verlag. Source

Sabarinath B.,Meenakshi Ammal Dental College and Hospital | Sivaramakrishnan M.,Meenakshi Ammal Dental College and Hospital | Sivapathasundharam B.,Meenakshi Ammal Dental College and Hospital
Journal of Oral and Maxillofacial Pathology | Year: 2012

Objective: Giant cell fibromas (GCF) of the oral cavity are found predominantly in Caucasians and rarely in other races. This retrospective study was done to evaluate the clinicopathological features of GCFs in a sample of Indian population. Materials and Methods: 21 oral GCF cases were investigated from the year 1995 to 2010. Clinical data and microscopic features were reviewed and analyzed. Results: The mean age of patients at the time of diagnosis was 39years. Oral GCF occurred in patients between 6 and 67 years of age. The lesions were 4-17 mm in greatest dimension. GCF frequently has the provisional diagnosis of fibroma or papilloma. All tumors were treated by total surgical excision and no recurrence was reported. The consistent and diagnostic feature was the presence of large stellate giant cells, usually with one or two nuclei. Multinucleated giant cells were seen occasionally. These giant cells were most numerous in the connective tissue beneath the epithelium. Conclusion: Though there are distinct histopathologic features for GCF, its clinical presentation and prognosis are similar to the conventional fibroma/fibroepithelial polyp. Source

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