Meenakshi Academy of Higher Education and Research

Kānchipuram, India

Meenakshi Academy of Higher Education and Research

Kānchipuram, India
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Pandurangan K.,Meenakshi Academy of Higher Education and Research | Krishnappan V.,Madha Dental College and Hospitals | Subramanian V.,Meenakshi Academy of Higher Education and Research | Subramanyan R.,Sri Lakshminarayana Institute of Medical science
Inflammopharmacology | Year: 2015

Objective: The aim of the present study was to evaluate the anti-inflammatory effect of four dimethoxy flavone derivatives; 7,2′-dimethoxy flavone, 7,3′-dimethoxy flavone, 7,4′-dimethoxy flavone and 7,8,-dimethoxy flavone and to investigate the possible cellular mechanisms involved. Materials and methods: The acute anti-inflammatory effect of dimethoxy flavones was investigated by carrageenan induced hind paw oedema in rats. Further, the effect of dimethoxy flavones on certain mediators of pain and inflammation like cyclooxygenases (COX-1 and COX-2), pro-inflammatory cytokines (IL-1β and TNF-α) and free radical scavenging activity (NO and LPO) were investigated by using in vitro tests. Results: The investigated dimethoxy flavones produced a significant, dose and time dependent reduction of carrageenan induced paw oedema in rats with a maximum inhibition of 52.4 % observed for 7,4′-dimethoxy flavone. Although, the test compounds inhibited both the isoforms of cyclooxygenase, a higher degree of inhibition on COX-2 was evident. A concentration dependent inhibition of other inflammatory cytokines like tumor necrosis factor-α and interleukin-1β was identified in the present study. 7,4′-dimethoxy flavone was found to be maximally effective in inhibiting nitrite ion free radical generation and 7,8-dimethoxy flavone was more active in inhibiting lipid peroxidation than the other compounds. Conclusion: The results of the present study reveal the anti-inflammatory action of the investigated dimethoxy flavones. Inhibition of cyclooxygenases, cytokines and reactive oxygen species, observed in subsequent experiments may be suggested as possible mechanisms involved in the action of these compounds. © 2015, Springer Basel.


Pandurangan K.,Meenakshi Academy of Higher Education and Research | Krishnappan V.,Madha Dental College and Hospitals | Subramanian V.,Meenakshi Academy of Higher Education and Research | Subramanyan R.,Sri Lakshminarayana Institute of Medical science
European Journal of Pharmacology | Year: 2014

The aim of the present study was to evaluate the antinociceptive action of certain dimethoxy flavones (DMF, (7,2'-dimethoxy flavone, 7,3'-dimethoxy flavone, 7,4'-dimethoxy flavone and 7,8,-dimethoxy flavone) and the possible mechanisms involved. The antinociceptive effect of dimethoxy flavones was investigated in mice employing acetic acid-induced abdominal writhings, formalin-induced nociception and hot water tail immersion assay procedures. To identify the possible mechanisms involved in the antinociceptive action of these compounds, acetic acid-induced abdominal constriction assay alone was employed. Mice were pretreated with naloxone, yohimbine, ondansetron, haloperidol, bicuculline or glibenclamide before dimethoxy flavone treatment to identify the role of opioid, adrenergic, 5HT3-serotonergic, dopaminergic, gamma-amino butyric acid (GABA) receptor or potassium channels, respectively. The investigated dimethoxy flavones produced a significant reduction in the number of abdominal constrictions in acetic acid assay. A dose dependent decrease in paw-licking response time was evident in both the early and late phases of formalin induced nociception. A significant increase in reaction time was also evident after treatment with various dimethoxy flavones in hot water tail immersion assay. Pretreatment with naloxone, ondansetron or glibenclamide significantly attenuated the antinociceptive effect of all the four dimethoxy flavones. Yohimbine pretreatment attenuated the antinociceptive response of 7,3'-dimethoxy flavone, 7,4'-dimethoxy flavone and 7,8-dimethoxy flavone. Pretreatment with haloperidol potentiated the antinociceptive response of all the tested dimethoxy flavones. The antinociceptive effect of 7,2'-dimethoxy flavone and 7,3'-dimethoxy flavone was annulled by bicuculline pretreatment. The results of the present study reveal the antinociceptive effect of dimethoxy flavones involving multiple pathways. © 2014 Elsevier B.V.


Chinnaraj K.,Bharathiar University | Manikandan A.,Presidency College at Chennai | Ramu P.,Meenakshi Academy of Higher Education and Research | Antony S.A.,Presidency College at Chennai
Journal of Superconductivity and Novel Magnetism | Year: 2014

Spinel iron oxide (Fe3O4) nanostructures were synthesized by a simple microwave-assisted combustion method (MACM) using glycine as the fuel. For the purpose of comparative study, it was also prepared by a simple sol-gel-assisted combustion method (SACM). The as-prepared samples were characterized by powder X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, high-resolution scanning electron microscopy (HR-SEM), energy dispersive X-ray spectroscopy (EDX), UV-Visible diffuse reflectance spectroscopy (DRS), photoluminescence (PL) spectroscopy, and vibrating sample magnetometry (VSM) techniques. XRD and EDX results confirmed that the as-prepared products were pure spinel Fe3O4 without any impurity. Average crystallite size is found to be 12.15 and 28.47 nm for MACM and SACM products, respectively. HR-SEM analysis shows that nanospheres (NSs) and nanoparticles (NPs) have like morphologies by MACM and SACM methods, respectively. Band-gap (Eg) energy of the sample NSs is 2.44 eV, whereas the sample NPs have 2.19 eV. Magnetic hysteresis (M-H) loops revealed that the as-prepared Fe3O4 samples have superparamagnetic behavior at room temperature with saturation magnetization (Ms) values in the range of 38.53 and 46.51 emu/g for the sample NSs and NPs, respectively. The oxidation of benzyl alcohol into benzaldehyde reached a maximum of 67.43 % for NSs, whereas for NPs, the conversion was only 59.67 % with 100 % selectivity. © 2014, Springer Science+Business Media New York.


Yuvaraja T.,Meenakshi Academy of Higher Education and Research | Gopinath M.,NGP
Asian Journal of Pharmaceutical and Clinical Research | Year: 2015

The modern heuristic techniques mainly include the application of the artificial intelligence approaches such as genetic algorithm, particle swarm optimization algorithm, ant colony optimization, stochastic diffusion search, differential evolution, etc. The main aspect of these techniques is their flexibility for solving the optimization problems that have different mathematical constraints. In a power system area, the competition between the electric utilities is gradually increased due to the deregulation of the electrical markets. For this reason, the generation expansion problem presents itself as an important issue that needs to be considered in order to achieve reasonable economic decisions. © 2015, Asian Journal of Pharmaceutical and Clinical Research. All rights reserved.


PubMed | Meenakshi Academy of Higher Education and Research, Sri Lakshminarayana Institute of Medical science and Madha Dental College and Hospitals
Type: Journal Article | Journal: Inflammopharmacology | Year: 2015

The aim of the present study was to evaluate the anti-inflammatory effect of four dimethoxy flavone derivatives; 7,2-dimethoxy flavone, 7,3-dimethoxy flavone, 7,4-dimethoxy flavone and 7,8,-dimethoxy flavone and to investigate the possible cellular mechanisms involved.The acute anti-inflammatory effect of dimethoxy flavones was investigated by carrageenan induced hind paw oedema in rats. Further, the effect of dimethoxy flavones on certain mediators of pain and inflammation like cyclooxygenases (COX-1 and COX-2), pro-inflammatory cytokines (IL-1 and TNF-) and free radical scavenging activity (NO and LPO) were investigated by using in vitro tests.The investigated dimethoxy flavones produced a significant, dose and time dependent reduction of carrageenan induced paw oedema in rats with a maximum inhibition of 52.4% observed for 7,4-dimethoxy flavone. Although, the test compounds inhibited both the isoforms of cyclooxygenase, a higher degree of inhibition on COX-2 was evident. A concentration dependent inhibition of other inflammatory cytokines like tumor necrosis factor- and interleukin-1 was identified in the present study. 7,4-dimethoxy flavone was found to be maximally effective in inhibiting nitrite ion free radical generation and 7,8-dimethoxy flavone was more active in inhibiting lipid peroxidation than the other compounds.The results of the present study reveal the anti-inflammatory action of the investigated dimethoxy flavones. Inhibition of cyclooxygenases, cytokines and reactive oxygen species, observed in subsequent experiments may be suggested as possible mechanisms involved in the action of these compounds.


Borra S.K.,Frontier Lifeline Hospital | Mahendra J.,Meenakshi Ammal Dental College | Gurumurthy P.,Meenakshi Academy of Higher Education and Research | Jayamathi,Meenakshi Ammal Dental College | And 2 more authors.
Journal of Clinical and Diagnostic Research | Year: 2014

Background: Among various reactive oxygen species, hydroxyl radicals have the strongest chemical activity, which can damage a wide range of essential biomolecules such as lipids, proteins, and DNA.Objective: The objective of this study was to investigate the beneficial effects of curcumin on prevention of oxidative damage of biomolecules by hydroxyl radicals generated in in vitro by a Fenton like reaction.Materials and Methods: We have incubated the serum, plasma and whole blood with H2O2/Cu2 +/ Ascorbic acid system for 4 hours at 37 0C and observed the oxidation of biomolecules like albumin, lipids, proteins and DNA.Results: Curcumin at the concentrations of 50,100 and 200 μmoles, prevented the formation of ischemia modified albumin, MDA, protein carbonyls, oxidized DNA and increased the total antioxidant levels and GSH significantly.Conclusion: These observations suggest the hydroxyl radical scavenging potentials of curcumin and protective actions to prevent the oxidation of biomolecules by hydroxyl radicals. © 2014, Journal of Clinical and Diagnostic Research. All rights reserved.


Rama Krishna Reddy Y.V.,Frontier Lifeline Hospital | Mahendra J.,Meenakshi Ammal Dental College | Gurumurthy P.,Meenakshi Academy of Higher Education and Research | Jayamathi,Meenakshi Ammal Dental College | Babu S.,Frontier Lifeline Hospital
Journal of Clinical and Diagnostic Research | Year: 2015

Introduction: Although the cardiovascular disease (CVD) burden is rising in different countries, the morbidity and mortality rate is not reduced to much extent because of lack of application of the biomarkers for diagnosing CVD. Hence, we aimed to establish the predictable biomarkers in conjunction to framingham risk score in order to predict the risk for CVD in non cardiac patients.Materials and Methods: Three hundred subjects were screened for the study who came for the master health checkup. Out of them 50 patients were excluded as they were under medication. 23 patients were excluded due to various systemic diseases like fever and infection etc. The remaining of 227 patients with age range of 30-80 y was randomly selected for investigation. These subjects were divided into four different groups: Group I – controls with age range: 30-60 y (n=50) these subjects were free from all the systemic ailments and risk factors. Study groups comprised of Group II - (n=44) with age range: 30-40 y, Group III - (n=50) with age range: 41-50 y and Group IV - (n=83) with age range: 51-80 y. Patients with different risk factors without medication participated as study groups. Routine biochemical parameters were analysed using fully automated analyser and atherosclerotic biomarkers was analysed using ELISA kit. In addition to this, framingham risk scores was calculated in all the groups, for 30 y risk prognosis for CVD.Results: The atherosclerotic biomarkers such as E-selectin, Leptin, osteoprotegerin (OPG) and Ox-LDL were elevated among the study groups as compared to control group. Pearson correlation showed a significant association between the individual risk score (30 y framingham risk for CVD) of individuals, and the above biomarkers. The Receiver operating curve (ROC) analysis also showed a greater area under curve with higher sensitivity and specificity.Conclusion: We conclude the application E-Selectin, leptin, OPG and Ox-LDL as biomarkers along with the framingham risk scores in prediction risk for CVD in the individuals with subclinical atherosclerosis. It is more reliable and predictable as compared to the individual biomarkers alone. © 2015, Journal of Clinical and Diagnostic Research. All rights reserved.


PubMed | Meenakshi Academy of Higher Education and Research
Type: Journal Article | Journal: European journal of dentistry | Year: 2017

The purpose of this study was to assess the role of cross section on cyclic fatigue resistance of One Shape, Revo-S SU, and Mtwo rotary files in continuous rotation and reciprocating motion in dynamic testing model.A total of 90 new rotary One Shape, Revo-S SU, and Mtwo files (ISO size 25, taper 0.06, length 25 mm) were subjected to continuous rotation or reciprocating motion. A cyclic fatigue testing device was fabricated with 60 angle of curvature and 5 mm radius. The dynamic testing of these files was performed using an electric motor which permitted the reproduction of pecking motion. All instruments were rotated or reciprocated until fracture occurred. The time taken for each instrument to fracture was recorded. All the fractured files were analyzed under a scanning electron microscope (SEM) to detect the mode of fracture. Statistical analysis was performed using one-way ANOVA, followed by Tukeys honestly significant difference The time taken for instruments in reciprocating motion to fail under cyclic loading was significantly longer when compared with groups in continuous rotary motion. There was a statistically significant difference between Mtwo rotary and the other two groups in both continuous and reciprocating motion. One Shape rotary files recorded significantly longer duration to fracture resistance when compared with Revo-S SU files in both continuous and reciprocating motion. SEM observations showed that the instruments of all groups had undergone a ductile mode of fracture.Reciprocating motion improved the cyclic fatigue resistance of all tested groups.


PubMed | Meenakshi Academy of Higher Education and Research
Type: Comparative Study | Journal: Journal of endodontics | Year: 2014

The purpose of this study was to evaluate and compare the cyclic fatigue resistance of RaCe (FKG Dentaire, La Chaux-de-Fonds, Switzerland) and Mtwo (VDW, Munich, Germany) rotary files in continuous rotation and reciprocating motion.A total of 60 new rotary Mtwo and RaCe files (ISO size = 25, taper = 0.06, length = 25 mm) were selected and randomly divided into 4 groups (n = 15 each): Mtc (Mtwo NiTi files in continuous rotation), Rc (RaCe NiTi files in continuous rotation), Mtr (Mtwo NiTi files in reciprocating motion), and Rr (RaCe NiTi files in reciprocating motion). A cyclic fatigue testing device was fabricated with a 60 angle of curvature and a 5-mm radius. All instruments were rotated or reciprocated until fracture occurred. The time taken for each instrument to fracture and the length of the broken fragments were recorded. All the fractured files were analyzed under a scanning electron microscope to detect the mode of fracture. The Kolmogorov-Smirnov test was used to assess the normality of samples distribution, and statistical analysis was performed using the independent sample t test.The time taken for the instruments of the Mtr and Rr groups to fail under cyclic loading was significantly longer compared with the Mtc and Rc groups (P < .001). Scanning electron microscopic observations showed that the instruments of all groups had undergone a ductile mode of fracture. The length of the fractured segments was between 5 and 6 mm, which was not statistically significant among the experimental groups.Mtwo and RaCe rotary instruments showed a significantly higher cyclic fatigue resistance in reciprocating motion compared with continuous rotation motion.


PubMed | Meenakshi Academy of Higher Education and Research, Frontier Lifeline Hospital and Meenakshi Ammal Dental College
Type: Journal Article | Journal: Journal of clinical and diagnostic research : JCDR | Year: 2015

Although the cardiovascular disease (CVD) burden is rising in different countries, the morbidity and mortality rate is not reduced to much extent because of lack of application of the biomarkers for diagnosing CVD. Hence, we aimed to establish the predictable biomarkers in conjunction to framingham risk score in order to predict the risk for CVD in non cardiac patients.Three hundred subjects were screened for the study who came for the master health checkup. Out of them 50 patients were excluded as they were under medication. 23 patients were excluded due to various systemic diseases like fever and infection etc. The remaining of 227 patients with age range of 30-80 y was randomly selected for investigation. These subjects were divided into four different groups: Group I - controls with age range: 30-60 y (n=50) these subjects were free from all the systemic ailments and risk factors. Study groups comprised of Group II - (n=44) with age range: 30-40 y, Group III - (n=50) with age range: 41-50 y and Group IV - (n=83) with age range: 51-80 y. Patients with different risk factors without medication participated as study groups. Routine biochemical parameters were analysed using fully automated analyser and atherosclerotic biomarkers was analysed using ELISA kit. In addition to this, framingham risk scores was calculated in all the groups, for 30 y risk prognosis for CVD.The atherosclerotic biomarkers such as E-selectin, Leptin, osteoprotegerin (OPG) and Ox-LDL were elevated among the study groups as compared to control group. Pearson correlation showed a significant association between the individual risk score (30 y framingham risk for CVD) of individuals, and the above biomarkers. The Receiver operating curve (ROC) analysis also showed a greater area under curve with higher sensitivity and specificity.We conclude the application E-Selectin, leptin, OPG and Ox-LDL as biomarkers along with the framingham risk scores in prediction risk for CVD in the individuals with subclinical atherosclerosis. It is more reliable and predictable as compared to the individual biomarkers alone.

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