Medizinisches Labor Ostsachsen

Görlitz, Germany

Medizinisches Labor Ostsachsen

Görlitz, Germany
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Nenoff P.,Haut und Laborarzt Allergologie andrologie | Schubert K.,Hautarztpraxis | Jarsumbeck R.,Medizinisches Labor Ostsachsen | Uhrlass S.,Haut und Laborarzt Allergologie andrologie | Kruger C.,Haut und Laborarzt Allergologie andrologie
Aktuelle Dermatologie | Year: 2017

Following a holiday in Egypt, a 28 year old woman developed erythematosquamous, pustular and purulent abcessing lesions over the mons pubis, in the groin, and the lower abdomen. Suspecting a dermatomycosis, topical antifungal treatment was initiated. Microbiological diagnostic yielded fast growing, white-yellowish pigmented, granular fungus as well as Staphylococcus aureus and B-streptococci. Morphological differentiation revealed Trichophyton (T.) mentagrophytes variatio asteroides, a zoophilic dermatophyte. Treatment of the kerion-like dermatomycosis of the pubo-genital region with oral terbinafine failed to improve the appearance of the skin lesions. A short treatment change to fluconazole and antibiotic treatment e. g. with sultamicillin also failed to show improvement of the infection. Eventually the patient with tinea genitalis profunda was treated over 4.5 months with terbinafine 250 mg daily until complete resolution occurred. The dermatosis resulted in Brocq’s pseudopélade. The species identification of the dermatophyte isolate using sequencing of the ITS region of the rDNA yielded a 100 % agreement with sequences of zoophilic T. mentagrophytes isolates deposited at the National Center for Biotechnology Information (NCBI), Bethesda, Maryland. The DNA sequence of this variety matches with current T. mentagrophytes strains in Germany originating from patients returning from Thailand and suffering from abscess forming tinea genitalis. An animal source for the infection could not be identified. An indirect transmission through contamination in wellness and fitness aspects of the hotel in Egypt is probable. Shaving of the intimate area, which the patient continued to do during the entire duration of the treatment, is seen as predisposing factor. To what extent the higher virulence of zoophilic T. mentagrophytes strains found in Germany (“Thai variant”) plays a role, in comparison to other strains of this species, should be investigated in experimental studies. © Georg Thieme Verlag KG Stuttgart · New York.


Zhao S.,TU Munich | Dorn J.,TU Munich | Napieralski R.,TU Munich | Napieralski R.,Therawis Diagnostics GmbH | And 9 more authors.
Biological Chemistry | Year: 2017

In serous ovarian cancer, the clinical relevance of tumor cell-expressed plasmin(ogen) (PLG) has not yet been evaluated. Due to its proteolytic activity, plasmin supports tumorigenesis, however, angiostatin(-like) fragments, derived from PLG, can also function as potent anti-tumorigenic factors. In the present study, we assessed PLG protein expression in 103 cases of advanced high-grade serous ovarian cancer (FIGO III/IV) by immunohistochemistry (IHC). In 70/103 cases, positive staining of tumor cells was observed. In univariate Cox regression analysis, PLG staining was positively associated with prolonged overall survival (OS) [hazard ratio (HR)=0.59, p=0.026] of the patients. In multivariable analysis, PLG, together with residual tumor mass, remained a statistically significant independent prognostic marker (HR=0.49, p=0.009). In another small patient cohort (n=29), we assessed mRNA expression levels of PLG by quantitative PCR. Here, elevated PLG mRNA levels were also significantly associated with prolonged OS of patients (Kaplan-Meier analysis; p=0.001). This finding was validated by in silico analysis of a microarray data set (n=398) from The Cancer Genome Atlas (Kaplan-Meier analysis; p=0.031). In summary, these data indicate that elevated PLG expression represents a favorable prognostic biomarker in advanced (FIGO III/IV) high-grade serous ovarian cancer. © 2017 Walter de Gruyter GmbH, Berlin/Boston.


Nenoff P.,Labor fur medizinische Mikrobiologie | Schorlemmer B.,Institute For Medizinische Diagnostik Greifswald | Uhrlass S.,Labor fur medizinische Mikrobiologie | Baunacke A.,Hautarztpraxis Dr. Annegret Baunacke | And 6 more authors.
Hautarzt | Year: 2016

Moulds or non-dermatophyte moulds (NDM) are being increasingly isolated as causative agent of onychomycoses. Known causes of a NDM-OM are Scopulariopsis brevicaulis, Fusarium, Aspergillus, Acremonium, Neoscytalidium dimidiatum, Arthrographis kalrae, and Chaetomium. In this article, 5 patients with suspected nail infection due to Onychocola canadensis are reported for the first time in Germany. Systemic antifungal agents are not considered to be effective in NDM onychomycosis. In individual cases, however, terbinafine seems to be effective in Onychocola canadensis infection of the nails. Treatment of choice represents, however, nontraumatic nail avulsion using 40 % urea ointment followed by antifungal nail lacquer with ciclopirox olamine or amorolfine. © 2016 Springer-Verlag Berlin Heidelberg


PubMed | Hautarztpraxis Dr. Jens Ifflander, Bruker, Hautarztpraxis Dr. Barbara und Ellen Syhre, Institute For Medizinische Diagnostik Greifswald and 4 more.
Type: Journal Article | Journal: Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete | Year: 2016

Moulds or non-dermatophyte moulds (NDM) are being increasingly isolated as causative agent of onychomycoses. Known causes of a NDM-OM are Scopulariopsis brevicaulis, Fusarium, Aspergillus, Acremonium, Neoscytalidium dimidiatum, Arthrographis kalrae, and Chaetomium. In this article, 5 patients with suspected nail infection due to Onychocola canadensis are reported for the first time in Germany. Systemic antifungal agents are not considered to be effective in NDM onychomycosis. In individual cases, however, terbinafine seems to be effective in Onychocola canadensis infection of the nails. Treatment of choice represents, however, nontraumatic nail avulsion using 40% urea ointment followed by antifungal nail lacquer with ciclopirox olamine or amorolfine.


Bruchmann S.,Helmholtz Center for Infection Research | Muthukumarasamy U.,Helmholtz Center for Infection Research | Pohl S.,Helmholtz Center for Infection Research | Preusse M.,Helmholtz Center for Infection Research | And 8 more authors.
Environmental Microbiology | Year: 2015

Health-care-associated infections by multi-drug-resistant bacteria constitute one of the greatest challenges to modern medicine. Bacterial pathogens devise various mechanisms to withstand the activity of a wide range of antimicrobial compounds, among which the acquisition of carbapenemases is one of the most concerning. In Klebsiella pneumoniae, the dissemination of the K. pneumoniae carbapenemase is tightly connected to the global spread of certain clonal lineages. Although antibiotic resistance is a key driver for the global distribution of epidemic high-risk clones, there seem to be other adaptive traits that may explain their success. Here, we exploited the power of deep transcriptome profiling (RNA-seq) to shed light on the transcriptomic landscape of 37 clinical K. pneumoniae isolates of diverse phylogenetic origins. We identified a large set of 3346 genes which was expressed in all isolates. While the core-transcriptome profiles varied substantially between groups of different sequence types, they were more homogenous among isolates of the same sequence type. We furthermore linked the detailed information on differentially expressed genes with the clinically relevant phenotypes of biofilm formation and bacterial virulence. This allowed for the identification of a diminished expression of biofilm-specific genes within the low biofilm producing ST258 isolates as a sequence type-specific trait. © 2015 Society for Applied Microbiology and John Wiley & Sons Ltd.


PubMed | Medizinisches Labor Ostsachsen, Charité - Medical University of Berlin and Helmholtz Center for Infection Research
Type: Journal Article | Journal: Environmental microbiology | Year: 2015

Health-care-associated infections by multi-drug-resistant bacteria constitute one of the greatest challenges to modern medicine. Bacterial pathogens devise various mechanisms to withstand the activity of a wide range of antimicrobial compounds, among which the acquisition of carbapenemases is one of the most concerning. In Klebsiella pneumoniae, the dissemination of the K. pneumoniae carbapenemase is tightly connected to the global spread of certain clonal lineages. Although antibiotic resistance is a key driver for the global distribution of epidemic high-risk clones, there seem to be other adaptive traits that may explain their success. Here, we exploited the power of deep transcriptome profiling (RNA-seq) to shed light on the transcriptomic landscape of 37 clinical K. pneumoniae isolates of diverse phylogenetic origins. We identified a large set of 3346 genes which was expressed in all isolates. While the core-transcriptome profiles varied substantially between groups of different sequence types, they were more homogenous among isolates of the same sequence type. We furthermore linked the detailed information on differentially expressed genes with the clinically relevant phenotypes of biofilm formation and bacterial virulence. This allowed for the identification of a diminished expression of biofilm-specific genes within the low biofilm producing ST258 isolates as a sequence type-specific trait.


Taubert H.,Martin Luther University of Halle Wittenberg | Wurl P.,University of Ulm | Greither T.,Martin Luther University of Halle Wittenberg | Kappler M.,Martin Luther University of Halle Wittenberg | And 8 more authors.
British Journal of Cancer | Year: 2010

Background: The urokinase plasminogen activator (uPA) system is one of the best-investigated protease systems, both under physiological and pathological conditions, including various types of cancer. However, effects of co-expression of members of the uPA system in soft-tissue sarcoma (STS) patients at the protein level in both tumour tissue and serum have not been investigated yet. Methods:We examined 82 STS patients for protein levels of uPA, PAI-1and uPAR in tumour tissue and serum by ELISA.results: A significant correlation between high antigen levels of uPA, PAI-1 or uPAR in tumour tissue, and of uPAR in serum, with poor outcome of STS patients was found for the first time. Most strikingly, we observed an additive effect of combined uPA, PAI-1 or uPAR levels in tumour tissue extracts with uPAR levels in serum on patients prognosis. High uPA/uPAR, PAI-1/uPAR and uPAR/uPAR antigen levels in tumour tissue/serum were associated with a 5.9-fold, 5.8-fold and 6.2-fold increased risk of tumour-related death (P0.003, 0.001 and 0.002, respectively) compared with those patients who displayed low levels of the respective marker combination.Conclusion:As expression of members of the uPA system in tumour tissue and serum is additively correlated with prognosis of STS patients, our results suggest that combinations of these biomarkers can identify STS patients with a higher risk of tumour-related death. © 2010 Cancer Research UK All rights reserved.


Grismayer B.,TU Dresden | Sato S.,TU Munich | Kopitz C.,TU Munich | Ries C.,Ludwig Maximilians University of Munich | And 8 more authors.
Biological Chemistry | Year: 2012

mRNA levels of the urokinase receptor splice variant uPAR-del4/5 are associated with prognosis in breast cancer. Its overexpression in cancer cells affects tumor biologically relevant processes. In the present study, individual breast cancer cell clones displaying low vs. high uPAR-del4/5 expression were analyzed demonstrating that uPAR-del4/5 leads to reduced cell adhesion and invasion in a dose-dependent manner. Additionally, matrix metalloproteinase-9 (MMP-9) was found to be strongly upregulated in uPAR-del4/5 overexpressing compared to vector control cells. uPAR-del4/5 may thus play an important role in the regulation of the extracellular proteolytic network and, by this, influence the metastatic potential of breast cancer cells. © 2012 Walter de Gruyter GmbH, Berlin/Boston.


Grismayer B.,TU Munich | Solch S.,TU Munich | Seubert B.,TU Munich | Kirchner T.,Medizinisches Labor Ostsachsen | And 8 more authors.
Molecular Cancer | Year: 2012

Background: Rab proteins constitute a large family of monomeric GTP-binding proteins that regulate intracellular vesicle transport. Several Rab proteins, including rab31, have been shown to affect cancer progression and are related with prognosis in various types of cancer including breast cancer. Recently, the gene encoding rab31 was found to be overexpressed in estrogen receptor-positive breast cancer tissue. In a previous study we found a significant association of high rab31 mRNA expression with poor prognosis in node-negative breast cancer patients. In the present study, we aimed to investigate the impact of rab31 (over)-expression on important aspects of tumor progression in vitro and in vivo.Methods: Breast cancer cells displaying low (MDA-MB-231) or no (CAMA-1) endogenous rab31 expression were stably transfected with a rab31 expression plasmid. Batch-transfected cells as well as selected cell clones, expressing different levels of rab31 protein, were analyzed with regard to proliferation, cell adhesion, the invasive capacity of tumor cells, and in vivo in a xenograft tumor model. Polyclonal antibodies directed to recombinantly expressed rab31 were generated and protein expression analyzed by immunohistochemistry, Western blot analysis, and a newly developed sensitive ELISA.Results: Elevated rab31 protein levels were associated with enhanced proliferation of breast cancer cells. Interestingly, weak to moderate overexpression of rab31 in cell lines with no detectable endogenous rab31 expression was already sufficient to elicit distinct effects on cell proliferation. By contrast, increased expression of rab31 in breast cancer cells led to reduced adhesion towards several extracellular matrix proteins and decreased invasive capacity through MatrigelTM. Again, the rab31-mediated effects on cell adhesion and invasion were dose-dependent. Finally, in a xenograft mouse model, we observed a significantly impaired metastatic dissemination of rab31 overexpressing MDA-MB-231 breast cancer cells to the lung.Conclusions: Overexpression of rab31 in breast cancer cells leads to a switch from an invasive to a proliferative phenotype as indicated by an increased cell proliferation, reduced adhesion and invasion in vitro, and a reduced capacity to form lung metastases in vivo. © 2012 Grismayer et al.; licensee BioMed Central Ltd.


Kotzsch M.,TU Dresden | Bernt K.,TU Dresden | Friedrich K.,TU Dresden | Luther E.,TU Dresden | And 7 more authors.
Histopathology | Year: 2010

Aims: The urokinase-type plasminogen activator receptor (uPAR) is a key molecule for pericellular proteolysis in tumour cell invasion and metastasis. The aim was to evaluate the prognostic impact of uPAR in invasive breast cancer dependent on which cell types within the tumour express uPAR. Methods and results: uPAR expression was analysed by immunohistochemistry in 270 tumour tissue specimens of invasive ductal breast carcinomas using tissue microarrays. For evaluation of uPAR immunoexpression we used the epitope-mapped, uPAR domain II-specific monoclonal antibody IID7. High uPAR score values in both tumour cells (uPAR-Tc) and stromal cells were significantly related to high tumour grade (G3), and inversely correlated with oestrogen receptor status. On multivariate analysis, high uPAR-Tc values contributed independent prognostic information for disease-free survival (hazard ratio 1.93, P = 0.007) when adjusted for prognostically relevant clinicopathological parameters, whereas uPAR expression in stromal cells was not related to prognosis. In addition, elevated uPAR-Tc values were found to be prognostic indicators in clinically relevant subgroups of patients with invasive breast cancer. Conclusions: In invasive breast cancer uPAR expression in invasive carcinoma cells, but not in stromal cells, has a significant impact on patients' prognosis, and contributes to a more aggressive tumour phenotype. © 2010 Blackwell Publishing Limited.

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