Kind B.,Medizinische Fakultat Carl Gustav Carus |
Muster B.,TU Darmstadt |
Staroske W.,TU Dresden |
Herce H.D.,Rensselaer Polytechnic Institute |
And 6 more authors.
Human Molecular Genetics | Year: 2014
RibonucleaseH2plays an essential role forgenomestability as itremoves ribonucleotides misincorporated into genomic DNA by replicative polymerases and resolves RNA/DNA hybrids. Biallelic mutations in the genes encoding the three RNase H2 subunits cause Aicardi-Goutie`res syndrome (AGS), an early-onset inflammatory encephalopathy that phenotypically overlaps with the autoimmune disorder systemic lupus erythematosus. Here we studied the intracellular dynamics of RNase H2 in living cells during DNA replication and in response to DNA damage using confocal time-lapse imaging and fluorescence cross-correlation spectroscopy. We demonstrate that the RNase H2 complex is assembled in the cytosol and imported into the nucleus in an RNase H2Bdependent manner. RNase H2 is not only recruited to DNA replication foci, but also to sites of PCNA-dependent DNA repair. By fluorescence recovery after photobleaching, we demonstrate a high mobility and fast exchange of RNase H2 at sites of DNA repair and replication.We provide evidence that recruitment of RNase H2 is not only PCNA-dependent, mediated by an interaction of theBsubunit with PCNA, but also PCNA-independentmediated via the catalytic domain of the A subunit. We found that AGS-associated mutations alter complex formation, recruitment efficiency and exchange kinetics at sites of DNA replication and repair suggesting that impaired ribonucleotide removal contributes to AGS pathogenesis. © The Author 2014. Published by Oxford University Press. All rights reserved.