Mediteknology Srl

Bologna, Italy

Mediteknology Srl

Bologna, Italy
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Viol I.,CNR Institute of Neuroscience | Viol I.,University of Rome La Sapienza | Palama I.E.,CNR Institute of Neuroscience | Coluccia A.M.L.,University of Salento | And 12 more authors.
Integrative Biology (United Kingdom) | Year: 2013

We have recently reported initial results concerning an original approach to introduce additional properties into fibrillar proteins produced by live fibroblasts and extruded into the ECM. The key to such an approach was biocompatible, fluorescent and semiconducting synthetic molecules which penetrated spontaneously the cells and were progressively encompassed via non-bonding interactions during the self-assembly process of the proteins, without altering cell viability and reproducibility. In this paper we demonstrate that the intracellular secretion of fluorescent microfibers can be generalized to living primary and immortalized human/mouse fibroblasts. By means of real-time single-cell confocal microscopy we show that the fluorescent microfibers, most of which display helical morphology, are generated by intracellular coding of the synthetic molecules. We also describe co-localization experiments on the fluorescent microfibers isolated from the cell milieu demonstrating that they are mainly made of type-I collagen. Finally, we report experimental data indicating that the embedded synthetic molecules cause the proteins not only to be fluorescent but also capable of electrical conductivity © The Royal Society of Chemistry 2013.

Agency: European Commission | Branch: H2020 | Program: MSCA-RISE | Phase: MSCA-RISE-2016 | Award Amount: 1.24M | Year: 2017

Investing in energy efficiency and saving is crucial to support energy accessibility and environmental protection, and it is the worlds best interest to share and implement forms of energy efficiency. This implies a stronger and effective transnational policy to promote and disseminate know-how about new technologies both at the market and R&D level. In this respect, development of projects centered on energy efficient technologies based on nanostructured organic materials certainly is a strategic field. The progresses in mastering organic matter by self-assembly and self-organization to form ordered soft-materials revolutionized the field opening new frontiers for both fundamental and applied research. However the route towards organic materials for application at the industrial scale is restricted by difficulties in the control and manipulation of the structural organization at the molecular level and its manifestation at higher scales. Motivated by the potential for significant energy savings, the INFUSION project aims to create a strongly interdisciplinary and inter-sectorial environment in which the principles of self-organization are poured from the Academia into the private sector and vice-versa to create new paradigms to engineer electrochromic devices. Through a detailed plan of 83 secondments, the project aims at cross-fertilize the electrochromic technology joining specific expertise to realize a bottom-up approach toward the design, preparation and characterization of self-organized organic materials (chromophores, CNSs, polymers...) at different interfaces (ITO, graphene) and exhibiting superior performances (optical, durability). For the transfer of knowledge, the project combines the multidisciplinary expertise of 6 universities, 1 research institute, and 3 companies representing 4 EU and 2 TC countries (Pakistan and Argentina) in the field of organic chemistry, photochemistry, surface science, polymer and materials science, and device engineering.

Sotgiu G.,CNR Institute for Organic Syntheses and Photoreactivity | Galeotti M.,Mediteknology Srl | Samori C.,CNR Institute for Organic Syntheses and Photoreactivity | Bongini A.,University of Bologna | Mazzanti A.,University of Bologna
Chemistry - A European Journal | Year: 2011

The design and synthesis of new fluorescent dyes with emission range at 490-650 nm are described. Their structural and electronic properties have been characterized by both experimental techniques and quantum-chemical calculations. The chromophores are donor-π-bridge-acceptor push-pull compounds with a π bridge of phenyl and thiophene rings and their combination. Compared with previous thiophene fluorophores, these dyes show significant redshift in the absorption and emission spectra and offer compact, red-emitting fluorophores. The dyes have amino succinimidyl active ester and can be readily conjugated to proteins, polymers and other amino-group-containing materials. Making light of things: A new series of bright, thiophene-based fluorescent dyes has been synthesized. The push-pull strategy allowed easy and fine tuning of emission peaks over a wide spectral range from the visible to deep red (see image for two examples). Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Palama I.E.,CNR Institute of Neuroscience | Di Maria F.,University of Salento | Di Maria F.,CNR Institute for Organic Syntheses and Photoreactivity | D'Amone S.,CNR Institute of Neuroscience | And 5 more authors.
Journal of Materials Chemistry B | Year: 2015

Live cells can form multifunctional and environmentally responsive multiscale assemblies of living and non-living components. We recently reported the results of a unique approach to introduce supplementary properties, fluorescence in particular, into fibrillar proteins produced by live fibroblasts and extruded into the ECM. In this work, we demonstrate that the physiological secretion of fluorescent nanostructured microfibers upon the spontaneous uptake of the appropriate fluorophore extends to living cells derived by different tissue contexts. We also show that live cells seeded on fluorescent microfibers have a different fate in terms of the cellular morphology, cytoskeleton rearrangement and viability. These results suggest that the microfibers, which are biocompatible and biodegradable, can be used as multiscale biomaterials to direct the cell behaviour. This journal is © 2015 The Royal Society of Chemistry.

Melucci M.,CNR Institute for Organic Syntheses and Photoreactivity | Melucci M.,CNR Institute of Chemistry of organometallic Compounds | Zambianchi M.,CNR Institute for Organic Syntheses and Photoreactivity | Barbarella G.,CNR Institute for Organic Syntheses and Photoreactivity | And 11 more authors.
Journal of Materials Chemistry | Year: 2010

Oligothiophenes (TFs) with blue, green and orange emission have been used for the first time as doping fluorophores of silica nanoparticles (SiO 2NPs). High purification of the new nanoparticles (TFsSiO 2NPs) from free molecular fluorophores was achieved by means of asymmetrical flow field-flow fractionation on-line combined with multi-angle light scattering and fluorescent detection (AF4-MALS-FD). The synthesis, structural, compositional and optical characterizations of the new TFsSiO 2NPs are reported. We show that the tailored co-assembly of TFs in bi- and tricomponent TFsSiO2NPs allows for the fine-tuning of the emission of the nanoparticles from blue to white by means of FRET processes between adjacent TFs. These unique optical signatures make TFsSiO2NPs potentially effective tools for fluorescent sensing and labeling. © 2010 The Royal Society of Chemistry.

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