Medinat Srl Clinic

Camerano, Italy

Medinat Srl Clinic

Camerano, Italy

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Gil-del Valle L.,Institute of Tropical Medicine | de la C. Milian L.,Laboratories LIORAD | Toledo A.,Center for Molecular Immunology | Avila J.,Center for Pharmacoepidemiology Development | And 4 more authors.
Biomedicine and Preventive Nutrition | Year: 2011

Aims: To determine and compare an extensive array of biochemical redox indices before and after insulin type change in diabetes mellitus (DM) patients' type 1. Methods: Glutathione, malondialdehyde, peroxidation potential, superoxide dismutase, catalase, total hydroperoxide and advanced oxidation protein products in relation to blood glucose and glucose indicators control such as glycosylated haemoglobin and fructosamine were measured in 40 patients before and after 2 months since pork insulin was changed to human insulin in type 1 diabetic patients. These data was compared to sex and age-matched healthy control. Results: After 2 months of changing, all indicator measured were favorably evolved and were significantly modified (P< 0.05) except activity of erythrocyte enzymes superoxide dismutase and catalase. Adverse reactions (hypoglycaemic events) were observed in seven patients with 0.7% of incidence. It was related to concomitant use of captopril, clortalidone and nitropental for hypertension treatment. Simultaneous beneficial change in glucose, glucose control indices and redox markers were noted in 27 (68%) of total patients of study. Conclusion: These results contribute to both evidences that transition process from animal to human insulin could be beneficial to DM type 1 patients and an integral overview about metabolic events involved could be valid for evaluate treatment effects. The results contribute to evidences that transition to human insulin can improve the antioxidant status of diabetic patients. © 2011 Elsevier Masson SAS.


Delgado-Roche L.,University of Habana | Martinez-Sanchez G.,Medinat srl Clinic | Diaz-Batista A.,National Institute of Angiology and Vascular Surgery | Re L.,Medinat srl Clinic | Re L.,Marche Polytechnic University
International Journal of Ozone Therapy | Year: 2011

Coronary artery disease is considered a major cause of death in the western world and its primary pathological manifestation is myocardial damage due to ischemia-reperfusion phenomena. Ozone has been used as a therapeutic agent and beneficial effects against the damage induced by renal or hepatic ischemia-reperfusion have been observed in vivo. The present study evaluated the behaviour of oxidative stress biomarkers in coronary artery disease patients after 20 sessions of ozone (50 μg/mL ; 200 mL) by rectal insufflation. Blood samples of 40 patients and 50 healthy subjects were tested by spectrophotometric techniques. Indicators of biomolecular damage, enzymatic antioxidant activity and total antioxidant status were determined. We demonstrated that ozone therapy reduced the oxidative stress index as reflected by the increase in superoxide dismutase and catalase activities, the reduction of malondialdehyde, total hydroperoxides and advanced oxidation protein products concentration. Furthermore, an increase in glutathione levels was noted. The results of the present study show that repeated administrations of ozone in non-toxic doses play a positive role in the control of antioxidant/pro-oxidant balance in coronary artery disease patients.


Delgado-Roche L.,University of Habana | Martinez-Sanchez G.,Medinat srl Clinic | Re L.,Medinat srl Clinic | Re L.,Marche Polytechnic University
Journal of Cardiovascular Pharmacology | Year: 2013

ABSTRACT:: Atherosclerosis is a major cause of death in the Western World. It is known that Lipofundin 20% induces atherosclerotic lesions, whereas ozone at low doses has been satisfactorily used in the prevention of oxidative stress-associated pathologies, such as coronary artery diseases. The aim of the present work was to evaluate the effects of ozone therapy on Lipofundin-induced atherosclerotic lesions in New Zealand White rabbits. Ozone (1 mg), mixed with oxygen as passive carrier, was administered by rectal insufflation during 15 sessions in 5 weeks. Then, the animals were intravenously treated with 2 mL/kg of Lipofundin, daily during 8 days. Animals were euthanized and eosin and hematoxylin staining was used for aortic histopathological analysis. The biomarkers of oxidative stress and lipid profile in serum were determined by spectrophotometric techniques. The results demonstrated that ozone induced inhibitory effects on aortic lesions formation. On the other hand, a reduction of biomolecular damage and an increase of antioxidant systems were observed at the end of the experiment. The serum lipids profiles were not modified after only 1 cycle of ozone treatment. Our results reinforced the hypotheses that antioxidant effects induced by ozone in the context of atherosclerosis demonstrate the antiatherogenic properties of the gas in the experimental conditions of this study. Copyright © 2013 by Lippincott Williams & Wilkins.


Nabil Mawsouf M.,Cairo University | El-Sawalhi M.M.,Cairo University | Martinez-Sanchez G.,Medinat Srl Clinic | Darwish H.A.,Cairo University | And 2 more authors.
International Journal of Ozone Therapy | Year: 2010

Controlled ozone administration has been shown to promote an adaptation to oxidative stress by increasing endogenous antioxidant systems. The present study investigated the effects of O2-O3 administration either prophylactically or therapeutically on the alterations of oxidant status in adjuvant-induced arthritis in rats. Seven groups of rats were used: 1) normal control group; 2) control arthritic group (21 days); 3) prophylactic ozone group: arthritic rats received fifteen intra-rectal applications of O 2-O3 at 0.5, 0.7 and 1 mg/kg b.w. in a 5-6 mL volume starting one day before adjuvant inoculation and continued as five applications/week over 21 days; 4) oxygen group: received oxygen (vehicle of ozone) in a similar schedule to group 3; 5) control arthritic group (24 days); 6) therapeutic-ozone group: arthritic rats received ten intrarectal applications of O2-O3 at 0.5, 0.7 and 1 mg/kg b. w. in a 5-6 mL volume daily for ten days starting 14 days after adjuvant inoculation; 7) oxygen-treated group: received oxygen in a similar schedule to group 6. The effect of O2-O3 administration was assessed by measuring: blood glutathione (GSH), erythrocyte glutathione peroxidase and catalase activities, serum levels of protein thiols (PrSH), malondialdehyde (MDA) and nitrite/nitrate (NO2-NO3), as well as serum ceruloplasmin activity (CP). The present study showed that adjuvant-induced arthritis in rats caused a significant (p<0.05) reduction in blood GSH, serum PrSH levels and erythrocyte antioxidant enzyme activities accompanied by a significant (p<0.05) increase in serum levels of MDA, NO2-NO3 and CP activity. Ozone administration either prophylactically or therapeutically normalized blood GSH, serum PrSH and MDA levels and restored erythrocyte antioxidant enzyme activities. However ozone did not significantly (p>0.05) modify serum NO2-NO3 level in induced rats but significantly (p<0.05) increased CP activity. In conclusion, NO 2-NO3 oxidative preconditioning / postconditioning effectively modulated the antioxidant/oxidant balance associated with adjuvant arthritis model in rats.


Martinez-Sanchez G.,Medinat Srl Clinic | Re L.,Medinat Srl Clinic | Re L.,Marche Polytechnic University
International Journal of Ozone Therapy | Year: 2012

The rectal administration of ozone is one of the oldest systemic and local forms of application. The biological effects of the Rectal Insufflations of Ozone (RIO3) has been demonstrated extensively either experimentally or clinically. Furthermore, preclinical studies demonstrated its low toxicity. RIO3 has been now extended to treat many diseases and is increasingly being used as a systemic therapeutic form. RIO3 is already being viewed as an alternative to Mayor autohemotherapy (MAH). Using standardized clinical protocols a therapeutic success can be reached with RIO3. Handling the advantage and disadvantage of RIO3, not as alternative to MAH but used properly (e.g. pediatric, geriatric, when MAH cannot be performed because i.v. is difficult due to unfavorable vein conditions, etc.), this method is a valid route of O3/O2 administration.


Guevara-Garcia M.,Biopharmaceutical and Chemical Group LABIOFAM | Valle L.G.-D.,Institute Pedro Kouri | Martinez-Sanchez G.,Medinat Srl Clinic | Velasquez-Perez L.,Center For The Research And Rehabilitation Of Hereditary Ataxias Carlos nlay
Biomedicine and Aging Pathology | Year: 2012

Spinocerebellar ataxia 2 (SCA2) is a clinically, pathologically and genetically neurodegenerative disorder. The number of clinical assays in these patients is limited because, among other factors, a lack of biomarkers for the assessment of the main clinical and genetic features of the disease and the evaluation of therapeutic options. The aim of this study was to investigate an extensive array of redox status indexes: glutathione (GSH), malondialdehyde (MDA), peroxidation potential, superoxide dismutase (SOD), ferric reducing ability of plasma (FRAP), catalase (CAT), total hydroperoxide (TH) and advanced oxidation protein products (AOPP) by spectophotometric techniques in relation to electrophysiogical markers in-Cubans SCA2 patients compared to those of healthy aged-gender matched control. The Pearson correlation between progression markers and oxidative stress indexes were assessed too. Nineteen SCA2 patients' and 19 healthy subjects were recruited. Significant differences (P < 0.05) in PP, FRAP, GSH, SOD, AOPP and TH in SCA2 values were found relatively to the control group. The grade of oxidative reaction was evaluated as moderate to severe in almost markers for the studied group. Pearson significant correlation was found between PPLatMOS and FRAP-Lat. These results contribute both to the evidences that oxidative stress occurs during spinocerebellar ataxia type 2 and its useful for the integral management of patients. © 2012 Elsevier Masson SAS. All Rights Reserved.


Re L.,Marche Polytechnic University | Re L.,Medinat srl Clinic | Martinez-Sanchez G.,Medinat srl Clinic | Perez-Davison G.,Medinat srl Clinic | Sirito M.,Thermal Institute of Angolo Terme
International Journal of Ozone Therapy | Year: 2010

Basic fibroblast growth factor (bFGF) is a pleiotropic mitogen which plays an important role in cell growth, differentiation, migration and survival in different cells and organ systems. Application of bFGF has been shown to promote cellular proliferation and collagen synthesis in vivo. FGF is markedly up-regulated following bone or tendon injury and active at multiple stages of the healing stimulation process, local blood circulation, lipolysis and smooth muscle. Looking at the physical and chemical properties of the ozone molecule, the present work deals with its possible therapeutic action as an FGF activator. Incubation (2 h) of platelet-rich plasma with 80 μg/mL O 2/O 3 increases the basal concentration of FGF by approximately 600%. This fact in combination with previous demonstration of the stimulating action of O 3, releasing other platelet factors may potentially allow autologous treatment in aesthetic and clinical tissues conditions in which FGF has a leading role. The versatility and broad beneficial effect of ozone has become evident in orthopedics, cutaneous and mucosal infections as well as in dentistry. The induction of FGF and other growth factors by ozone can support and potentiate those applications.


Martinez-Sanchez G.,Medinat srl Clinic | Perez-Davison G.,Medinat srl Clinic | Re L.,Medinat srl Clinic | Re L.,Marche Polytechnic University | Giuliani A.,Univ.of Milan
Dose-Response | Year: 2011

Redox environment involves a broad network of pro-oxidant and antioxidant components. Health benefit or damage can be induced as a consequence of an adaptive cellular stress response. A consequence of hormetic amplification is an increase in the homeodynamic space of a living system in terms of an increased defense capacity and a reduced load of damaged macromolecules. Ozone, when used at appropriate doses, promotes the formation of reactive oxygen species and lipid peroxides allows them to become late and long-lasting messengers. Healthy aging may be achieved by hormesis through mild and periodic, but not severe or chronic, physical and mental challenges, and by the use of nutritional hormesis incorporating mild stress-inducing molecules called hormetins. The paradoxical concept that ozone eventually induces an antioxidant response capable of reversing a chronic oxidative stress is common in the animal and vegetal kingdom; it is already supported by findings of an increased level of antioxidant enzymes during ozone therapy. Those facts can include ozone as a hormetin. The established scientific foundations of hormesis are ready to pave the way for new and effective approaches in redoxrelated disease research and intervention; ozone therapy can be a good candidate. © 2011 University of Massachusetts. © 2011 University of Massachusetts.


PubMed | Medinat srl Clinic
Type: Journal Article | Journal: Dose-response : a publication of International Hormesis Society | Year: 2011

Redox environment involves a broad network of pro-oxidant and antioxidant components. Health benefit or damage can be induced as a consequence of an adaptive cellular stress response. A consequence of hormetic amplification is an increase in the homeodynamic space of a living system in terms of an increased defense capacity and a reduced load of damaged macromolecules. Ozone, when used at appropriate doses, promotes the formation of reactive oxygen species and lipid peroxides allows them to become late and long-lasting messengers. Healthy aging may be achieved by hormesis through mild and periodic, but not severe or chronic, physical and mental challenges, and by the use of nutritional hormesis incorporating mild stress-inducing molecules called hormetins. The paradoxical concept that ozone eventually induces an antioxidant response capable of reversing a chronic oxidative stress is common in the animal and vegetal kingdom; it is already supported by findings of an increased level of antioxidant enzymes during ozone therapy. Those facts can include ozone as a hormetin. The established scientific foundations of hormesis are ready to pave the way for new and effective approaches in redox-related disease research and intervention; ozone therapy can be a good candidate.

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