Kulkarni A.K.,MediCiti Institute of Medical science |
Ojha R.P.,University of Gorakhpur
Journal of Biomolecular Structure and Dynamics | Year: 2015
Solution conformation of the cyclic hexapeptide sequence, [cyclo-S-Cys-Tyr-Ile-Gln-Asn-Cys-S] (CYIQNC) - a disulfide-linked fragment of a neurohypophyseal peptide hormone oxytocin (OT) - has been investigated by high-field one-dimensional (1D) and two-dimensional (2D) NMR spectroscopic methods and compared with the results obtained from computer simulation studies. 1H-NMR results based on temperature dependence of amide proton chemical shifts and nuclear Overhauser effect indicate that peptide in solution populates different conformations, characterized by two fused β-turns. The segment Ile3-Gln4-Asn5-Cys6 yields a preferred type-III β-turn at residues 4, 5 (HB, 3HN → 6CO), while the segment Cys6, Cys1-Tyr2-Ile3 exhibits inherently weaker, flexible β-turn either of type I/II'/III/half-turn at residues 1, 2 (HB, 6HN → 3CO). The computer simulation studies using a mixed protocol of distance geometry-simulated annealing followed by constrained minimization, restrained molecular dynamics, and energy minimization showed the possibility of existence of additional conformations with the hydrogen bonds, (a) 5HN → 3CO and (b) 2HN → 6CO. These results, therefore, indicate that the additional conformations obtained from both NMR and simulation studies can also be possible to the peptide. These additional conformations might have very small population in the solution and did not show their signatures in these conditions. These findings will be helpful in designing more analogs with modifications in the cyclic moiety of OT. © 2014 Taylor & Francis.
Srinivasulu K.,Mediciti Institute of Medical science
Indian Journal of Forensic Medicine and Toxicology | Year: 2014
The study on assessment of Medical certification of cause of death was conducted at a rural medical college hospital in Andhra Pradesh to find out the errors in cause of death certificates for a period of one year from 1st January 2011 to 31st December 2011. The certificates are collected from Medical record department of the hospital along with case sheets and analysed for errors. The errors are grouped in to two Major and Minor, Not even a single certificate is free from error, majority of the errors an average about 47% are found in Major group and minor group consist 21% of errors, Most of the errors are avoidable with proper guidance and periodical training to the medical practitioners. © 2014, Indian Journal of Forensic Medicine and Toxicology. All rights reserved.
Kamtane R.A.,MediCiti Institute Of Medical science |
Jayawardhani V.,MediCiti Institute Of Medical science
Asian Journal of Pharmaceutical and Clinical Research | Year: 2012
Background: Reporting adverse drug reactions (ADRs) spontaneously is considered as a cornerstone of pharmacovigilance. However, its success depends on co-operative and motivated health care professionals. Under-reporting of the adverse drug reactions (ADRs) by the prescribers is a common problem. Objective: The present study is aimed at assessing the knowledge, attitude and perception of physicians from various fields in Hyderabad, towards adverse drug reactions reporting, to get an in-sight into the causes of under-reporting of ADRs and to suggest possible ways of improving this method of reporting. Method: The study was cross-sectional and questionnaire-based involving only medical doctors working in different fields. The completion of the questionnaire by respondents was taken as their consent to participate in the study. A total of 120 predesigned KAP (Knowledge, Attitude and Perception) questionnaires consisting of 25 questions were distributed to doctors with minimum qualification of MBBS. Microsoft Excel worksheet (Microsoft Office 2007) was used for statistical analysis. Result: Only 94 out of 120 respondents filled and returned the questionnaire within the stipulated time frame giving a response rate of about 78.33%. 88 [93.61%] doctors feel that ADR reporting and monitoring system would benefit the patients. Busy schedule, lack of knowledge about the exact authority to report ADRs, unavailability of ADR reporting forms, lack of incentives were some of the reasons for under-reporting of ADRs. Conclusion: It was observed that the knowledge of ADRs and how to report them are inadequate among doctors. More awareness should be created on the ADR reporting system.
Santhakumari R.,MediCiti Institute of Medical science |
Reddy I.Y.,MediCiti Institute of Medical science
International Journal of Pharma and Bio Sciences | Year: 2014
Cerebral metabolism will be affected in T2DM either by chronic hyperglycemia or by chronic hypoxia. Proton magnetic resonance spectroscopy (1H-MRS) of the brain provides detailed information about the structure, dynamics, reaction state and chemical environment of molecules. It also measures the levels of brain metabolites such as myoinositol (mI), N acetyl aspartate (NAA), creatine (Cr), choline (Cho), glutamate (Glu), glutamine (Gln) and gamma amino butyric acid (GABA). Several studies suggest that people with type 2 diabetes mellitus (T2DM) are at an increased risk of cognitive impairment in comparison with the general population. The altered metabolites may cause cognitive dysfunction in T2DM. This review article concludes that in T2DM, metabolite levels were altered in different regions of brain.
Ganguly E.,MediCiti Institute of Medical science
Online Journal of Health and Allied Sciences | Year: 2012
Reducing Total Fertility Rate (TFR) amongst rural Indian couples from the current level is a significant challenge to the population control policies relying solely on the Government efforts. REACH strategy, based on health metrics, succeeded in lowering the TFR below replacement levels in a rural population of more than 300,000 in Rajasthan. The REACH strategy was first developed and demonstrated success in decreasing TFR in a pilot project by SHARE India in Medchal region of Andhra Pradesh utilizing designated workers, and was replicated in Rajgarh District of Rajasthan in cooperation with Bhoruka Charitable Trust (supervisor of ICDS and NRHM health workers in Rajgarh) using Government health workers. The success of the REACH strategy in both Rajasthan and previously in Andhra Pradesh holds promise as a tool to reduce TFR in other areas of rural India.
Sureka R.K.,Mediciti Institute of Medical science |
Nandeshwar A.J.,Mediciti Institute of Medical science
International Journal of Pharma and Bio Sciences | Year: 2014
MRSA (Methicillin-resistant Staphylococcus aureus) is a major causative agent for hospital acquired infections. Prevalence of MRSA is increasing and leading to increased mortality, morbidity and cost of treatment due to increased number of days of hospital stay. So present study was conducted. Method: various clinical samples such blood urine, sputum, body fluids, BAL, wound swab and pus were collected under aseptic precautions and innoculated on bllod agar, MacConkeys agar and incubated overnight 37°c, once the growth was observed, bacterial identification was done by standard biochemical test and special test such as coagulase.mannitol fermentation confirms satphylococcus aureus. MRSA detuction done as per CLSI (Clinical and Laboratory Standards Institute) guidelines by cefoxitin disc diffusion method. Antibiotic sensitivity testing done by modified kirby baures disc diffusion method.out of 1224 samples, 371 were identified as staphylococcus aureus out of which 128 were found to be MRSA(methicillin resistant Staphylococcus aureus).prevalence of MRSA was 34.5%.Incidence and prevalence of MRSA is increasing at alarming rate, most of MRSA were found to be resistant ot commonly used antibiotics. Antibiotic policy and hand wash hygine are important to control hospital aquired infection by MRSA.
Samozai M.N.,MediCiti Institute of Medical science |
Kulkarni A.K.,MediCiti Institute of Medical science
Journal of Nutrition, Health and Aging | Year: 2015
Objectives: The frequent prescription of calcium supplements with vitamin D by health practitioners is a topic of concern globally. The present study was designed to find out whether the calcium supplements with vitamin D really affect serum and urinary calcium levels in post-menopausal women.Design, Settings & Participants: The age-matched comparative study was performed among postmenopausal women who were already on and those who were not on calcium supplements with vitamin D for a period of time in relation to the estimation of the serum calcium and the urine calcium levels. Sixty healthy postmenopausal women were enrolled, with thirty among them forming the study group (SG) — who were on calcium supplements with vitamin D for a period of a month, three months, and up to twelve months — were studied and compared the results obtained with the age-matched control group (CG) of thirty post-menopausal women who were not on calcium supplements.Measurements: The serum and the urinary calcium levels were estimated by using appropriate biochemical methods and the data were analysed using relevant statistical methods.Results: The serum calcium levels did not vary significantly in SG in spite of consuming calcium supplements over a period of time, whereas the urinary calcium levels increased progressively (p value < 0.005) in those who have taken calcium supplements for a year compared to those who have taken for a month.Conclusion: Thus, the calcium supplements were of little significance on the serum calcium levels, but have a significant effect on the urinary calcium levels in post-menopausal women. © 2014, Serdi and Springer-Verlag France.
Srinivasulu K.,Mediciti Institute of Medical science
Medico-Legal Update | Year: 2013
Prescription is one of the important Medico legal documents, the art of prescription writing has an ancient inheritance, commonly the prescription considered as a written order by the physician to the pharmacist to prepare and dispense the drugs. The term prescription means an order to take certain medications. Prescriptions have legal implications, prescriber is responsible for clinical care of the patient. Doctors are facing charges of medical negligence due to wrong prescriptions and illegible prescriptions, the number of cases filed in the courts are increasing day by day since from the inception of Consumer protection act in medical practise. A cross sectional study was done with an objective to appraise the importance and various medico legal aspects in prescription writing. The study was conducted at Ranga reddy Dist area in duration of one year, prescriptions were collected randomly from the patients and analysed for errors and deficiencies, in my study I observed too many lacunas in the prescriptions which can lead to medico legal problems.
Rekha C.,Mediciti Institute of Medical science
Indian journal of public health | Year: 2013
Accurate reporting of prevalence of low birth weight (LBW) is important for monitoring health of a population. LBW is often underestimated in developing countries due to heaping of the data at 2.5 kg. UNICEF uses an average adjustment factor of 25% to re-classify babies listed as exactly 2.5 kg into the LBW category. From October 2009 to February 2010, we weighed 859 consecutive live births at a rural hospital in Andhra Pradesh, India, using analog and digital scales to evaluate the relative validity of the adjustment factor. Significantly more babies weighed exactly 2.5 kg on analog (13.4%) versus digital (2.2%) scales, showing heaping. Percentage of LBW by digital method (29.5%) was significantly higher compared to the analog method (23%) and with adjustment factors (26.4%). Conventional methods of adjusting birth-weight data underestimate the prevalence of LBW. Sensitive digital weighing machines or better adjustment methods are needed to monitor LBW in developing countries.
Babu R.S.,Mediciti Institute of Medical science |
Kumar D.A.P.,Mediciti Institute of Medical science
International Journal of Pharma and Bio Sciences | Year: 2013
This study is an analysis of the patterns of variation of the human hand, particularly the metric characters of hand, palm, fingers, nails and dorsal distribution of hair over the digits. Anthropometric measurements were performed on 124 men and women, aged 21 to 41 years in and around medchal town. The data were analyzed by inferential statistics. Size variation in fingers is indicated in the present study. There is component of variability between all fingers as a whole and distal phalanges, the variation is much between the thumb and other fingers. Sexual dimorphism is proven fact as menhave greater dimensions than women in length of the thumb than other fingers.