Saino E.,Medicine Section |
Saino E.,Center For Tissue Engineering Cit |
Grandi S.,Center For Tissue Engineering Cit |
Quartarone E.,Center For Tissue Engineering Cit |
And 11 more authors.
European Cells and Materials | Year: 2011
Bioactive glasses synthesized by the sol-gel technique possess many of the qualities associated with an ideal scaffold material for a bone graft substitute. In view of the potential clinical applications, we performed a detailed in vitro study of the biological reactivity of synthesized 58S bioactive glass containing-zinc, in terms of osteoblast morphology, proliferation, and deposition of a mineralized extracellular matrix (ECM). Human Sarcoma Osteoblast (SAOS-2) cells were used to i) assess cytotoxicity by lactate dehydrogenase (LDH) release and ii) evaluate the deposition of a calcified extracellular matrix by ELISA assay and quantitative RT-PCR (qRT-PCR). In comparison with pure silica and 58S, the 58S-Zn0.4 bioglass showed a significant increase in cellular proliferation and deposition of ECM components such as decorin, fibronectin, osteocalcin, osteonectin, osteopontin, type-I and -IIIcollagens. Calcium deposition was significantly higher than on pure silica and 58S samples. Also Alkaline phosphatase(ALP) activity and its protein content was higher with respect to pure silica and 58S. qRT-PCR analysis revealed the up-regulation of type-I collagen, bone sialoprotein and osteopontin genes. All together these results demonstratethe cytocompatibility of 58S-Zn0.4 bioglass and its capability to promote osteoblast differentiation.
Fortunati E.,University of Perugia |
Armentano I.,University of Perugia |
Zhou Q.,KTH Royal Institute of Technology |
Iannoni A.,University of Perugia |
And 9 more authors.
Carbohydrate Polymers | Year: 2012
Nanocomposite films were prepared by the addition of cellulose nanocrystals (CNCs) eventually surfactant modified (s-CNC) and silver (Ag) nanoparticles in the polylactic acid (PLA) matrix using melt extrusion followed by a film formation process. Multifunctional composite materials were investigated in terms of morphological, mechanical, thermal and antibacterial response. The nanocomposite films maintained the transparency properties of the PLA matrix. Thermal analysis showed increased values of crystallinity in the nanocomposites, more evident in the s-CNC based formulations that had the highest tensile Young modulus. The presence of surfactant favoured the dispersion of cellulose nanocrystals in the polymer matrix and the nucleation effect was remarkably enhanced. Moreover, an antibacterial activity against Staphylococcus aureus and Escherichia coli cells was detected for ternary systems, suggesting that these novel nanocomposites may offer good perspectives for food packaging applications which require an antibacterial effect constant over time. © 2011 Elsevier Ltd. All rights reserved.
Novikov Z.,Technion - Israel Institute of Technology |
Glover W.J.,Babson College |
Trepman P.C.,Stanford University |
Naveh E.,Technion - Israel Institute of Technology |
And 2 more authors.
Health Care Management Review | Year: 2016
Background: Integration between organizational units to achieve common goals has been of interest to health systems because of the potential to improve patient-centered care. However, the means by which integrative practices actually influence patient-centered care remain unclear. Whereas many studies claim a positive association between implementation of integrative practices and patient-centered care, others raise concerns that integrative practices may not necessarily improve patient-centered care. Purpose: The aim of this studywas to explore themechanismbywhich integrative practices influence patient-centered care and to suggest a systematic approach for effective integration. Approach: We conducted a qualitative study comparing diabetes and mental health services through focus groups with 60 staff members from one health maintenance organization.We developed quantitative indicators to support the suggested model. Findings: We identified a five-category framework of integrative practices that each directly and distinctively influences patient-centered care.Moreover, our findings suggest that integrative practices influence patient-centered care indirectly through creation of interdependent treatment competence, which enables providers to repeatedly deliver interdependent treatment in a flexible and adaptive way. Practical Implications: Providers should carefully implement integrative practices considering patient and disease characteristics, as our findings suggest that more implementation of integrative practices is not necessarily better. Copyright © 2016 Wolters Kluwer Health, Inc. All rights reserved.
Sutherland W.H.F.,Medicine Section |
de Jong S.A.,Medicine Section |
Hessian P.A.,University of Otago |
Williams M.J.A.,Medicine Section
Metabolism: Clinical and Experimental | Year: 2010
Circulating numbers of endothelial microparticles (EMP) are an index of endothelial injury and dysfunction; and microparticles positive to CD31 antibody increase acutely after cooked, fatty fast-food meals that are rich in saturated fatty acids (SAFA) and lipid oxidation products. The aim of this study was to determine the acute effect of meals rich in SAFA and native and thermally oxidized polyunsaturated vegetable oil on circulating numbers of EMP positive to CD144 antibody, a more specific marker of EMP. Twenty-two apparently healthy subjects received isocaloric meals rich in cream (CR), unheated sunflower oil, or heated sunflower oil in a randomized crossover study design. Circulating numbers of CD144-EMP and plasma lipids and Svedberg unit of flotation (Sf) greater than 400 triglyceride content were measured before and 1 and 3 hours after the meals. Triglycerides in the plasma Sf greater than 400 fraction increased significantly (P < .001) after the meals, with a significantly (P < .05) larger increase after the CR meal. Plasma CD144-EMP increased significantly (20%, P < .05) after the unheated sunflower oil and heated sunflower oil meals and did not increase significantly (P = .55) after the CR meal. This response was significantly different among the meals (P = .002) when first-visit fasting plasma glucose was a covariate. In conclusion, these data suggest that ingestion of meals rich in n-6 polyunsaturated vegetable oil irrespective of whether it has been mildly thermally oxidized may acutely alter the state of the vascular endothelium, resulting in increased shedding of CD144-EMP. The physiologic implications of these findings remain to be determined. © 2010 Elsevier Inc. All rights reserved.