Effect of a hyperlipidic diet rich in omegas 3, 6 and 9 on aberrant crypt formation in rat colonic mucosa [Efeito de uma dieta hiperlipídica rica em ômegas 3, 6 e 9 na formação de criptas aberrantes emmucosa cólica de ratos]
Burlamaqui I.M.B.,Federal University of Ceara |
Dornelas C.A.,Federal University of Ceara |
Valenca J.T.,Federal University of Ceara |
Mota D.M.C.,Federal University of Ceara |
And 4 more authors.
Acta Cirurgica Brasileira | Year: 2012
PURPOSE: To determine whether a hypercaloric and hyperlipidic diet enriched with polyunsaturated fatty acids infuences the formation of aberrant crypt foci (ACF) in colonic mucosa of Wistar rats treated with azoxymethane (AOM).METHODS: At eight weeks of life, the rats were assigned to four groups: Group I-standard diet (STD) not treated with AOM; Group II-hypercaloric and hyperlipidic diet (FED), not treated with AOM; Group III-STD, treated with AOM; Group IV-FED, treated with AOM. At 16 weeks, the animals were injected intraperitoneal with 0.9% saline solution (Group I and II) or AOM at 15mg/Kg (Groups III and IV) once a week for two weeks. Fifteen weeks later, the animals were euthanized.RESULTS: FED promoted weight gain in Groups II and IV compared to Groups I and III, respectively. The groups did not differ with regard to the total number of ACF. The Chi-square test revealed no predominance of the presence of foci with ≤4 crypts. However, foci with ≥5 crypts were proportionally more prevalent in Group III than in Group IV (p=0.043).CONCLUSION: The administration of polyunsaturated fatty acids did not interfere with the formation of aberrant crypt foci, but reduced ACF multiplicity, exercising an attenuating effect on carcinogenesis.
Nardi S.M.T.,Instituto Adolfo Lutz Regional Laboratory |
Cordeiro J.A.,Medicine School |
Monteiro H.L.,Paulista University
Leprosy Review | Year: 2011
Disability caused by leprosy may be associated with stigma. The aim of this work is to describe the degree of disability, quality of life and level of physical activity of individuals with leprosy and to identify possible correlations between these factors. Ninety-seven patients from two referral centres were studied. A complete medical history was taken and the World Health Organization degree of physical disability classification (WHO-DG), the International Physical Activity Questionnaire (IPAQ) and the Medical Outcome Study 36-item Short-form health Survey (SF36) were applied. The mean age of patients was 51 ± 14·9 years old; participants were predominantly men, married, unemployed, had concluded treatment and had had lepromatous leprosy. The WHO-DG and the level of physical activity (P-value = 0·36) were not correlated. The WHO-DG showed that 72·2% of patients had disabilities, 37·1% of whom performed vigorous physical activities. No significant association was observed between the WHO-DG and the domains of the QoL SF-36 except for functional capacity (P-value = 0·02); the physical capacity is generally 'very good' when individuals have no disabilities and 'bad' with severe disabilities. In conclusion, the WHO-DG of leprosy patients does not affect the level of physical activities or quality of life except functional capacity. There is no significant association between physical activities and quality of life in these individuals. © Lepra.
Escobar C.H.,Medicine School |
Escobar C.H.,National University of Colombia |
Chaparro O.,National University of Colombia
Stem Cells Translational Medicine | Year: 2016
Molecules of animal or bacterial origin, which pose a risk for zoonoses or immune rejection, are commonly used for extraction, culture, and cryopreservation of mesenchymal stem cells. There is no sequentialandorderly protocol for producinghumanadipose-derivedstemcells (hASCs) under xeno-free conditions. After standardizing a humanplatelet lysate (hPL) production protocol, four humanadipose tissue samples were processed through explants with fetal bovine serum (FBS)-supplemented or hPLsupplemented media for extracting the adipose-derived stem cells. The cells were cultivated in cell culture medium + hPL (5%) or FBS (10%). The cellular replication rate, immunophenotype, and differentiation potential were evaluated at fourth passage. Cellular viability was evaluated before and after cryopreservation of the cells, with an hPL-based solution compared with an FBS-based solution. The explants cultured in hPL-supplemented media showed earlier and faster hASC proliferation than did those supplemented with FBS. Likewise, cells grownin hPL-supplementedmedia showeda greater proliferation rate, without losing the immunophenotype. Osteogenic differentiation of xeno-free hASC was higher than the hASC produced in standard conditions. However, adipogenic differentiation was reduced in xeno-free hASC. Finally, the cells cryopreserved in an hPL-based solution showed a higher cellular viability than the cells cryopreserved in an FBS-based. In conclusion,wehave developed a complete xeno-free protocol for extracting, culturing, and cryopreserving hASCs that can be safely implemented in clinical studies. © AlphaMed Press 2016.
Ferraz Arruda P.F.,Urology Section |
Spessoto L.C.F.,Urology Section |
Godoy M.,Medicine School |
Pereira De Godoy J.,Medicine School
Urology Annals | Year: 2011
The case of a rare complication is reported of a 53-year-old patient with giant polycystic kidney (4250 g) that evolved with acute small bowel occlusion. The patient was submitted to surgery which identified that the intestinal occlusion was due to external compression of the intestinal loops. Excision of the mass solved the case.
Patch test standard series recommended by the Brazilian Contact Dermatitis Study Group during the 2006-2011 period [Bateria de testes padrão preconizada pelo Grupo Brasileiro de Estudos em Dermatite de Contato: Período de 2006 a 2011]
Tanaka G.M.,Dermatology Clinic of the Santa Casa de Sao Paulo |
Suzuki N.M.,Dermatology Clinic of the Santa Casa de Sao Paulo |
Lazzarini R.,Medicine School |
Lopes A.S.A.,Medicine School |
And 2 more authors.
Anais Brasileiros de Dermatologia | Year: 2013
A retrospective study was carried out between 2006-2011. Six hundred and eighteen patients with suspected allergic contact dermatitis underwent the standard patch test series recommended by the Brazilian Contact Dermatitis Research Group. The aim of our study was to evaluate the variation of positive patch-test results from standard series year by year. The most frequently positive allergens were: nickel sulfate, thimerosal and potassium bichromate. Decrease of positive patch-test results over the years was statistically significant for: lanolin (p=0.01), neomycin (p=0.01) and anthraquinone (p=0.04). A follow-up study should be useful in determining which allergens could be excluded from standard series, as they may represent low sensitization risk. © 2013 by Anais Brasileiros de Dermatologia.