Time filter

Source Type

Cao F.,Southwest University | Cao F.,Medicinal Plant R and D Center | Xia J.,Southwest University | Xia J.,Medicinal Plant R and D Center | And 12 more authors.
Yaoxue Xuebao | Year: 2016

Artemisinin is the first choice for malaria treatment. The plastidial MEP pathway provides 5-carbon precursors (IPP and its isomer DMAPP) for the biosynthesis of isoprenoid (including artemisinin) Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (HDR) is the last enzyme involved in the MEP pathway, which catalyzes HMBPP to form IPP and DMAPP. In this study, we isolated the full-length cDNA of HDR from Artemisia annua L. (AaHDR2) and performed functional analysis. According to gene expression analysis of AaHDR2 (GenBank: KX058541) and AaHDRl reported ever (GenBank: ADC84348.1) by qPCR, we found that AaHDRl and AaHDRl had much higher expression level in trichomes than that in roots, stems, leaves and flowers. AaHDR2 had much higher expression level in flowers than that in leaves. Further, the plant hormones such as MeJA and ABA respectively up-regulated the expression level of AaHDRl and AaHDR2 significantly, but GA3 up-regulated the expression level of AaHDR2 only. The gene expression analysis of AaHDRl and AaHDR2 showed that AaHDR2 had a greater contribution than AaHDRl to isoprenoid biosynthesis (including artemisinin). We used AaHDR2 for the following experiments. Bioinformatic analysis indicated that AaHDR2 belonged to the HDR family and the functional complementation assay showed that AaHDR2 did have the enzymatic function of HDR, using E. coli mutant MG1655ara<>HZDR as host cell. The subcellular localization assay showed that AaHDR2 fused with GFP at its N-terminal specifically targeted in chloroplasts. Finally, AaHDR2 was overexpressed in Arabidopsis thaliana. TheAaHDR 2-overexpressing plants produced the isoprenoids including chlorophyll a, chlorophyll b and carotenoids at significantly higher levels than the wild-type Arabidopsis plants. In summary, AaHDR2 might be a candidate gene for genetic improvement of the isoprenoid biosynthesis.


Meftahizade H.,Medicinal Plant Research Center | Lotfi M.,University of Tehran | Moradkhani H.,Plant Biotechnology
African Journal of Biotechnology | Year: 2010

Melissa officinalis L., due to its useful application in medicine, is being paid more attention. In order to establish a stable regeneration system with 4 landraces collected from different climate in Iran, major parameters such as regeneration rate, rooting percentage, shooting induction, proliferation rate, fresh and dry weight as a biomass of cells were investigated. Statistical analysis of results showed that BAP in combination with NAA had the highest regeneration in shoot tips explants. NAA in combination with IAA and kinetin had the best response to callus induction. Also 1 mgl/l NAA had a higher response to rooting than other auxins used. 2,4-D at 1.0 mg/l and BAP at 0.5 mg/l showed the highest production of fresh and dry weight, 5.48 and 0.407 g, respectively, that is approximately 20 times the initial weight of callus. 2,4-D (1 mg/l) and BAP (0.5 mg/l) had the highest cells number. © 2010 Academic Journals.


Sarkaki A.,Ahvaz Physiology Research Center | Sarkaki A.,Medicinal Plant Research Center | Fathimoghaddam H.,Ahvaz Physiology Research Center | Mansouri S.M.T.,Ahvaz Physiology Research Center | And 7 more authors.
Pakistan Journal of Biological Sciences | Year: 2014

Cerebral Hypoperfusion Ischemia (CHI) has important role in neuronal damage and behavioral deficits, including memory and Long-term Potentiation (LTP) impairment. Protective effects of Gallic Acid (GA) on memory, hippocampus LTP and cell viability were examined in permanent bilateral common carotid artery occlusion in rats. Animals were divided into 9 groups: Control (Cont); sham operated (Sho); Cerebral Hypoperfusion Ischemia (CHI); CHI received normal saline (CHI +Veh); CHI treated with different doses gallic acid (50,100, 200 mg kg-1 for 5 days before and 5 days after CHI induction, orally); CHI treated with phenytoin (50 mg kg-1, ip) (CHI+Phe); and sham operated received 100 mg kg-1, orally (Sho+GAl 00). CHI was induced by bilateral common carotid artery occlusion (2VO). Behavioral, electrophysiological and histological evaluations were performed. Data were analyzed by one-way and repeated measures ANOVA followed by tukey's post-hoc test. GA improved passive avoidance memory, hippocampal LTP and cell viability in hippocampus and cortex of ischemic rats significantly (p<0.01). The results suggest that gallic acid via its antioxidative and free radicals scavenging properties attenuates CHI induced behavioral and electrophysiological deficits and has significant protective effect on brain cell viability. Dose of 100 mg kg-1 GA has affected the ischemic but not intact rats and its effect was more potent significantly than phenytoin, a routine drug for ischemic subjects. © 2014 Asian Network for Scientific Information.


Uma Maheshwari P.,Medicinal Plant Research Center | Ram Babu M.,Medicinal Plant Research Center | Basha S.K.M.,Medicinal Plant Research Center
Vegetos | Year: 2012

Sri Lankamalleswara wild life sanctuary (also famous as Lankamala hills and forest) is situated in the Lankamalai hill ranges are about 30kms from Kadapa. The Lankamalleswara wild life sanctuary is rich with rare and endangered medicinal plants. The most predominant medicinal plant species found in this region are Pterocarpus marsupium, Pterocarpus santalinus and Santalum album along with the several rare medicinal plants which are essentially required in preparing ayurvedic, sidda and Unani medicine. The present study emphasizes the wealth of medicinal plants in Sri Lankamalleswara wild life sanctuary of Y.S.R. District in A.P., India.


Rambabu M.,Medicinal Plant Research Center | Uma Maheshwari P.,Medicinal Plant Research Center | Basha S.K.M.,Medicinal Plant Research Center
Vegetos | Year: 2012

Talakona is floristically rich area where plants of various categories are growing spontaneously in their natural habitat. It is located in Sri Venketeswara National Park, Chittoor District of Andhra Pradesh in India. With a 270 feet (82 m) water fall, it is the highest waterfall in the state. Talakona is known for wide variety of Medicinal Plants that have been in existence, identified and utilized since hundreds of years by local tribes. The present study aims at documentation of Medicinal Plants used by tribes for various purposes.

Loading Medicinal Plant Research Center collaborators
Loading Medicinal Plant Research Center collaborators