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Meftahizade H.,Medicinal Plant Research Center | Lotfi M.,University of Tehran
African Journal of Biotechnology

Melissa officinalis L., due to its useful application in medicine, is being paid more attention. In order to establish a stable regeneration system with 4 landraces collected from different climate in Iran, major parameters such as regeneration rate, rooting percentage, shooting induction, proliferation rate, fresh and dry weight as a biomass of cells were investigated. Statistical analysis of results showed that BAP in combination with NAA had the highest regeneration in shoot tips explants. NAA in combination with IAA and kinetin had the best response to callus induction. Also 1 mgl/l NAA had a higher response to rooting than other auxins used. 2,4-D at 1.0 mg/l and BAP at 0.5 mg/l showed the highest production of fresh and dry weight, 5.48 and 0.407 g, respectively, that is approximately 20 times the initial weight of callus. 2,4-D (1 mg/l) and BAP (0.5 mg/l) had the highest cells number. © 2010 Academic Journals. Source

Sarkaki A.,Ahvaz Physiology Research Center | Sarkaki A.,Medicinal Plant Research Center | Fathimoghaddam H.,Ahvaz Physiology Research Center | Mansouri S.M.T.,Ahvaz Physiology Research Center | And 7 more authors.
Pakistan Journal of Biological Sciences

Cerebral Hypoperfusion Ischemia (CHI) has important role in neuronal damage and behavioral deficits, including memory and Long-term Potentiation (LTP) impairment. Protective effects of Gallic Acid (GA) on memory, hippocampus LTP and cell viability were examined in permanent bilateral common carotid artery occlusion in rats. Animals were divided into 9 groups: Control (Cont); sham operated (Sho); Cerebral Hypoperfusion Ischemia (CHI); CHI received normal saline (CHI +Veh); CHI treated with different doses gallic acid (50,100, 200 mg kg-1 for 5 days before and 5 days after CHI induction, orally); CHI treated with phenytoin (50 mg kg-1, ip) (CHI+Phe); and sham operated received 100 mg kg-1, orally (Sho+GAl 00). CHI was induced by bilateral common carotid artery occlusion (2VO). Behavioral, electrophysiological and histological evaluations were performed. Data were analyzed by one-way and repeated measures ANOVA followed by tukey's post-hoc test. GA improved passive avoidance memory, hippocampal LTP and cell viability in hippocampus and cortex of ischemic rats significantly (p<0.01). The results suggest that gallic acid via its antioxidative and free radicals scavenging properties attenuates CHI induced behavioral and electrophysiological deficits and has significant protective effect on brain cell viability. Dose of 100 mg kg-1 GA has affected the ischemic but not intact rats and its effect was more potent significantly than phenytoin, a routine drug for ischemic subjects. © 2014 Asian Network for Scientific Information. Source

Uma Maheshwari P.,Medicinal Plant Research Center | Ram Babu M.,Medicinal Plant Research Center | Basha S.K.M.,Medicinal Plant Research Center

Sri Lankamalleswara wild life sanctuary (also famous as Lankamala hills and forest) is situated in the Lankamalai hill ranges are about 30kms from Kadapa. The Lankamalleswara wild life sanctuary is rich with rare and endangered medicinal plants. The most predominant medicinal plant species found in this region are Pterocarpus marsupium, Pterocarpus santalinus and Santalum album along with the several rare medicinal plants which are essentially required in preparing ayurvedic, sidda and Unani medicine. The present study emphasizes the wealth of medicinal plants in Sri Lankamalleswara wild life sanctuary of Y.S.R. District in A.P., India. Source

Rambabu M.,Medicinal Plant Research Center | Uma Maheshwari P.,Medicinal Plant Research Center | Basha S.K.M.,Medicinal Plant Research Center

Talakona is floristically rich area where plants of various categories are growing spontaneously in their natural habitat. It is located in Sri Venketeswara National Park, Chittoor District of Andhra Pradesh in India. With a 270 feet (82 m) water fall, it is the highest waterfall in the state. Talakona is known for wide variety of Medicinal Plants that have been in existence, identified and utilized since hundreds of years by local tribes. The present study aims at documentation of Medicinal Plants used by tribes for various purposes. Source

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