Ehrlichia canis detection in dogs from várzea grande: A comparative analysis of blood and bone marrow samples [Detecção de Ehrlichia canis em cães domiciliados em Várzea Grande: Análise comparativa entre amostras de sangue e medula óssea]
Makino H.,Federal University of Mato Grosso |
Sousa V.R.F.,Medicina Veterinaria e Zootecnia |
Fujimori M.,Medica Veterinaria Autonoma |
Rodrigues J.Y.,Medica Veterinaria Autonoma |
And 4 more authors.
Ciencia Rural | Year: 2016
The objective of this study was to compare the DNA detection of Ehrlichia canis in blood and bone marrow to determine the prevalence of the agent in Várzea Grande, Mato Grosso. Blood samples and bone marrow from 80 dogs of both sexes, different breeds and age, were collected and processed for a cross-sectional study performed using nested PCR. Of the 80 dogs, 61 (76.3%) had E. canis DNA in one of the samples. The buffy coat was positive in 42 dogs (52.5%) and the bone marrow was positive in 33 (41.3%). There was no significant association between the positive biological samples of either the buffy coat or bone marrow and the presence or absence of clinical signs (P=0.49). No risk factor was associated with infection in the studied area. The bone marrow samples were efficient for the molecular diagnosis of canine ehrlichiosis, particularly when there was a negative blood sample, although infection was present. © 2015, Universidade Federal de Santa Maria. All Rights Reserved.
Changes in chemical composition of whole fl ours from grains amaranth a fter different thermal treatments [Modifi cação da composição química de farinhas integrais de grãos de amaranto após a aplicação de diferentes tratamentos térmicos]
Bianchin M.G.A.,Federal University of Mato Grosso |
Beleia A.P.,State University Londrina |
Bianchini A.,Medicina Veterinaria e Zootecnia
Ciencia Rural | Year: 2014
The use of heat is an alternative to ensure the safety, improve sensory characteristics and increase digestibility of vegetables, making them suitable for children consumption. However, severe heat conditions may alter its nutritional composition. The objective of this study was to evaluate the effect of dry or humid heat before milling in chemical composition of whole amaranth (Amaranthus cruentus) grain fl ours. The fl ours were produced from in nature whole grains (UGF), toasted (PGF), cooking and dried (CDF) and popping (PGF). The heat treatmentsapplied to grains did not affect the mineral contents of the fl our, with the exception of Cu and Ca. However, the treatment elevated the levels of lipids (12-19%) and soluble fiber (18-35%) and reduced the insoluble fiber (5.8-8.5%) of the fl our when compared to in nature. The sulfur amino acids were fi rst limiting in UGF, for 1-10 years old and the popping reduced the quality of the amino acids which has become limiting, too, in lysine, for all ages. The toasting and cooking improved the balance of amino acids of fl ours obtained and the sulfur amino acids ceased to be the limiting for children over three years.
Melo A.L.T.,Federal University of Mato Grosso |
Da Silva G.C.P.,Federal University of Mato Grosso |
De Aguiar D.M.,Medicina Veterinaria e Zootecnia
Semina:Ciencias Agrarias | Year: 2012
The present study evaluated the prevalence of anti- Neospora caninum antibodies in 406 dogs from Cuiabá city, Mato Grosso State by means Immunofluorescence Assay (IFA) with cut-off at the initial dilution of 1:50. Twenty seven dogs were positive (6.6%, 95% CI%: 4.6-9.6%) with titers of antibodies ranging between 50 and 1600. According to the breed, Pit Bull dogs were the most frequent where 4 dogs (16.7%) were positive (P=0.06). There was no difference between positivity and age of dogs. From the positive dogs, 10 were females (5.6%) and 17 were males (7.5%), 7 dogs (9.0%) fed commercial ration and 20 (6.1%) homemade food (P > 0.05). Environmental variables showed that 15 positive dogs (7.5%) had access to the street, 21 positive dogs (7.5%) lived near to the wasteland, 20 (6.2%) lived in walled house and 20 (6.8%) had contact with rodents in house backyard. None of environmental variables had significant associations (P < 0.05). The results of the present study demonstrate a low prevalence of anti-N. caninum antibodies in canine population from Cuiabá city and the analyzed variable did not presented association for infection. The study noteworthy the lower prevalence of anti-N. caninum antibodies in urban area from Brazil.
De Amorim M.V.,Federal University of Mato Grosso |
Tome Melo A.L.,Federal University of Mato Grosso |
De Freitas A.L.S.,Medicina Veterinaria da UFMT |
Laub R.P.,Medicina Veterinaria da UFMT |
And 6 more authors.
Semina:Ciencias Agrarias | Year: 2013
This study evaluated the infection caused by Rickettsia spp. among dogs and horses from Mato Grosso State. A total of 384 dogs and 460 horses were tested by the Indirect Immunofluorescence Assay (IFA) for Rickettsia rickettsii, Rickettsia parkeri, Rickettsia amblyommii, Rickettsia rhipicephali and Rickettsia bellii Overall, 3.12% (12/384) of the dogs were seroreactive for at least one Rickettsiaof the other rickettsial antigens, allowing us to consider that these dogs were infected by R. bellii. In horses, 273 (59.34 %) samples were positives for at least one antigen tested, and highest anti-Rickettsia spp. endpoint titers were observed for R. amblyommii, suggesting homologous reactions to this agent or a very closely related organism. The results showed that dogs are under low exposition to ticks infected with spotted fever group Rickettsia, indicating low risk of human infection by these agents. However, R. amblyommii is widely distributed in the State.