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Reshkova V.,Medical University of Sofa | Kalinova D.,Medical University of Sofa | Rashkov R.,Medical University of Sofa
Biotechnology and Biotechnological Equipment | Year: 2013

Drug-related-lupus (DRL) is one of the manifestations of the drug-induced lupus (DIL). The aim of the present study was to analyze the clinical and immunological characteristics of patients with DRL, in order to summarize the manifestations, which worsen the prognosis of the disease. One hundred patients with systemic lupus erythematosus (SLE) were included in the study. Patients with DRL were 10% of all screened lupus patients. In the patients observed by us, DRL had developed after the use of Cephalosporin (Rocephin), D-penicillamine, gold salts, Procainamide, beta-blockers, vaccines. The leading clinical manifestations in these patients were cutaneous and muscular ones, respectively, necrotizing vasculitis in five patients (50%), myositis - in three patients (30%), and in two patients (20%) - generalized skin reaction, toxic epidermal necrolysis (Lyell syndrome). We found antinuclear antibodies (ANA) in nine patients (90%) with DRL. © Biotechnol. & Biotechnol.

Lyapina M.,Medical University of Sofa | Krasteva A.,Medical University of Sofa | Dencheva M.,Medical University of Sofa | Tzekova M.,Medical University of Sofa | And 2 more authors.
Biotechnology and Biotechnological Equipment | Year: 2013

Formaldehyde (F) is a ubiquitous chemical agent, part of our working and residential environment. It is a well-known mucous membrane irritant and a skin sensitizing agent associated with contact dermatitis. The concomitant exposure to formaldehyde and glutaraldehyde is common in dental practice. The aim of the present study was to perform a comparative overall evaluation of the health status of subjects with formaldehyde and glutaraldehyde-specific allergic contact dermatitis, in occupational and non-occupational exposure. A detailed and intentionally conducted questionnaire survey was performed among a total of 78 participants: 30 dental professionals occupationally exposed to formaldehyde, and 48 non-exposed healthy subjects (referents). All participants were clinically assessed, including a review of medical summary of history and physical examination. Skin patch testing with formaldehyde and glutaraldehyde was performed and a possible co-reactivity was assessed. The general incidence of formaldehyde-specific allergic contact dermatitis was 28.2%, and of glutaraldehyde-specific allergic contact dermatitis, 24.4%. The results demonstrated a stronger irritant effects of formaldehyde compared to glutaraldehyde, expressed in significantly higher incidence and relative risk of subjective upper respiratory tract (p = 0,029, OR=3,049 {1,102-8,442}), respiratory system (p = 0,002, OR=4,916 {1,699-14,228}) and skin (p = 0,019, OR=3,314 {1,191-9,218}) symptoms among subjects with formaldehyde-specific contact dermatitis. The gender analysis showed a significantly higher frequency and relative risk for sensitization to formaldehyde among women (χ2 = 4,371, p = 0.037, OR = 3,219 (1,053-9,838). Women with formaldehyde-specific allergic contact dermatitis seem to be a vulnerable group for clinical upper respiratory tract alterations. No increased incidence of upper respiratory tract infectious pathology among subjects with allergic contact dermatitis was established. Tobacco smoking seems to be a risk factor for sensitization to formaldehyde, but not to glutaraldehyde. Allergic predisposition is a risk factor for sensitization to glutaraldehyde, but not to formaldehyde. On other hand, its role for co-reactivity was categorically demonstrated. Studies of this kind, including patch-testing for more dental materials compounds, would be beneficial for early diagnosis of contact allergy, as well as risk assessment and management. © Biotechnol. & Biotechnol.

Shinkov A.,Medical University of Sofa | Borissova A.-M.,Medical University of Sofa | Kovatcheva R.,Medical University of Sofa | Atanassova I.,Medical University of Sofa | And 2 more authors.
Arquivos Brasileiros de Endocrinologia e Metabologia | Year: 2014

Objective: The aim of the study was to assess the prevalence and characteristics of metabolic syndrome (MetS) and its elements in relation to TSH in euthyroid subjects. Materials and methods: In the cross-sectional study, 2,153 euthyroid adults, 47.2 ± 14.5 years (20-94) with no current antithyroid or thyroid replacement therapy were enrolled. All participants filled a questionnaire on past and current morbidities, medication and smoking. Body weight, height, waist circumference, serum TSH, glucose and lipids were measured. The subjects were stratified by quartiles of TSH (QTSH) and the prevalence of the MetS elements was calculated. MetS was determined by the IDF 2005 criteria. Results: Overweight prevalence was 37.2% (35.2-39.2), obesity in 25.1% (23.3-26.9), abdominal obesity – 61.4% (59.3-63.5), hypertension – 42.1% (38.9-43.1), diabetes/increased fasting glucose – 13.6% (12.1-15), low HDL-cholesterol – 27.6% (25.7-29.5), hypertriglyceridemia – 24.1% (22.3-25.9), MetS – 32.2% (30.2-34.2). MetS was more prevalent in the highest QTSH (34.9%, 30.9-38.9) than the lowest (27%, 23.3-30.9), p < 0.001, as were low HDL-C (32%, 28-35.9 vs. 25%, 21.3-28.7, p < 0.001) and hypertriglyceridemia (26.8%, 23-30.5 vs. 20.4%, 17-23.8, p = 0.015). Each QTSH increased the risk of MetS by 14%, p < 0.001, of hypertriglyceridemia by 20%, p = 0.001 and of low LDL-C by 9%, p = 0.042. Other significant factors for MetS were age, male gender and obesity. Conclusion: The prevalence of MetS increased with higher QTSH within the euthyroid range, mostly by an increase in the dyslipidemia. © ABE&M todos os direitos reservados.

Hadjidekova S.P.,Medical University of Sofa | Avdjieva-Tzavella D.M.,Medical University of Sofa | Rukova B.B.,Medical University of Sofa | Nesheva D.V.,Medical University of Sofa | And 2 more authors.
Biotechnology and Biotechnological Equipment | Year: 2013

Molecular karyotyping is an extremely suitable method for genetic diagnosis of patients with unclear dysmorphic syndromes and intellectual disability. In this study we present our results from microarray analysis of 52 patients with developmental delay and congenital malformations. Our data revealed definite etiology in 9 out of 52 patients tested. Fifteen pathological aberrations were found in them. All pathological findings were validated by fluorescent in situ hybridization (FISH) analysis. In addition, the majority of the patients tested (41 patients) showed normal variations in the number of copies and variations of unknown clinical significance (34 patients). Analyses of the type and distribution of the different variations was performed and the clinical significance of variants of unknown nature was discussed. Our results show the advantages of high resolution microarrays for clinical diagnosis of patients with intellectual disability and congenital malformations, and also highlight the need for extensive population studies revealing the molecular nature and clinical significance of different copy number variations and for creation of detailed maps of variations in the Bulgarian population. © Biotechnol. & Biotechnol.

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