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Cechowska-Pasko M.,Medical University of Biaystok
Cell biology international | Year: 2011

We decided to study the effect of glucose deprivation on collagen metabolism in MCF7 cells. The incorporation of [3H]-proline into collagenase-sensitive and hydroxyproline-containing proteins was used as an index of collagen synthesis, whereas pulse-chase technique was employed to evaluate the degradation of newly synthesized proteins. The MCF7 cells incubated in high glucose medium synthesized detectable amounts of collagenous proteins. Most of them were found in the cell layer. The shortage of glucose resulted in about 30% reduction in collagen synthesis. The pulse-chase experiments demonstrated that proportionally less collagen was degraded in cultures incubated in low-glucose than in high-glucose media. Source

Janas R.M.,Childrens Memorial Health Institute | Rybak A.,Childrens Memorial Health Institute | Wierzbicka-Ruciska A.,Childrens Memorial Health Institute | Socha P.,Childrens Memorial Health Institute | And 10 more authors.
Gut and Liver | Year: 2016

Background/Aims: The roles of the many bioactive peptides in the pathogenesis of celiac disease remain unclear. To evaluate the serum concentrations of insulin, ghrelin, adiponectin, leptin, leptin receptor, and lipocalin-2 in children with celiac disease who do and do not adhere to a gluten-free diet (GFD, intermittent adherence). Methods: Prepubertal, pubertal, and adolescent celiac children were included in this study (74 girls and 53 boys on a GFD and 80 girls and 40 boys off of a GFD). Results: Insulin levels in prepubertal (9.01±4.43 IU/mL), pubertal (10.3±3.62 IU/mL), and adolescent (10.8±4.73 IU/mL) girls were higher than those in boys (5.88±2.02, 8.81±2.88, and 8.81±2.26 IU/mL, respectively) and were neither age-dependent nor influenced by a GFD. Prepubertal children off of a GFD exhibited higher ghrelin levels than prepubertal children on a GFD. Adiponectin levels were not age-, sex-nor GFD-dependent. Adherence to a GFD had no effect on the expression of leptin, leptin receptor, and lipocalin-2. Conclusions: Adherence to a GFD had no influence on the adiponectin, leptin, leptin receptor, and lipocalin-2 concentrations in celiac children, but a GFD decreased highly elevated ghrelin levels in prepubertal children. Further studies are required to determine whether increased insulin concentrations in girls with celiac disease is suggestive of an increased risk for hyperinsulinemia. Source

Tomczyk M.,Medical University of Biaystok | Tumanov A.,National Academy of Sciences of Belarus | Zaniewska A.,Medical University of Bialystok | Surazynski A.,Medical University of Bialystok
Planta Medica | Year: 2010

The effects of oxidative stress on collagen and DNA biosynthesis, β-galactosidase activity, the expression of the β-integrin receptor, FAK, the insulin-like growth factor-I receptor (IGFIR), the hypoxia-inducible factor-1 (HIF-1), and the mitogen-activated protein kinases (MAP/ERK 1, ERK2) were evaluated in human endometrial carcinoma cells. Subconfluent cells were subjected to oxidative stress with 30μ M t-butylhydroperoxide (t-BHP) for 1h per day over the course of 5 days. It was found that oxidative stress contributed to an increase in the β-galactosidase activity as well as to the inhibition of collagen and DNA biosynthesis. The mechanism of the process was found at the level of IGFIR and HIF-1. An increase in the expression of HIF-1 and a decrease in the expression of IGFIR were observed in the cells subjected to oxidative stress. The role of IGFIR signalling in the process was confirmed by an experiment showing downregulation of MAP kinases ERK1 and ERK2 expression in the studied cells. This phenomenon is probably responsible for the drastic inhibition of protein (up to 40% of control) and DNA biosynthesis (up to 65% of control) in the cells. An addition of tiliroside to the cells medium restored all parameters to the control level, including IGFIR and HIF-1 expressions. The data suggest that the antioxidative activity of tiliroside isolated from Potentilla argentea may originate at the level of IGFIR and HIF-1 signalling. © Georg Thieme Verlag KG Stuttgart - New York. Source

Krajewska-Kullak E.,Medical University of Biaystok | Kulak W.,Medical University of Bialystok | Marcinkowski J.T.,Poznan University of Medical Sciences | Damme-Ostapowicz K.V.,Medical University of Biaystok | And 4 more authors.
CIN - Computers Informatics Nursing | Year: 2011

The objective of this research was to assess Internet addiction among students of the Faculty of Health Prevention at the Medical University of Białystok. The present study included 358 students-nursing (n = 232), midwifery (n = 71), and medical rescue (n = 55). The following instruments were administered to the participants:the Young test, a test of the intensity of the abstinence syndrome, and a test of "online" addiction. Students who did not have a computer at home spent 3 hours a day on the Internet; students who did have a computer at home spent 0.5 to 8hours. On average, all respondents spent 1.8 ± 1.3 hours daily online. Internet addiction was confirmed among 24 (10.3%) nursing, 7 (9.9%) midwifery, and 5(9.1%) medical rescue students. The abstinence syndrome was noted among 11 (4.7%) nursing, 7(9.9%) obstetrics, and 7 (12.7%) medical rescue students. Several students had both an Internet addiction and the abstinence syndrome. Copyright © 2011 Wolters Kluwer Health | Lippincott Williams & Wilkins. Source

Jaroszewicz J.,Medical University of Bialystok | Flisiak-Jackiewicz M.,Medical University of Biaystok | Lebensztejn D.,Medical University of Biaystok | Flisiak R.,Medical University of Bialystok
Expert Opinion on Investigational Drugs | Year: 2015

Introduction: More than 100 million people worldwide are infected with hepatitis C virus (HCV), which is responsible for chronic liver disease accompanied by progressive fibrosis of hepatic tissue, often leading to liver cirrhosis. Novel therapeutic options are able to clear the virus in almost all diagnosed patients. However, even after successful treatment, hepatic fibrosis may persist in many of them. There is no registered therapy specific for liver fibrosis, but numerous molecules are currently in development.Areas covered: In this review, the authors look at drugs in early development for the treatment of HCV-related hepatic fibrosis. Their mechanism of action is based on the inhibition of hepatic inflammation, the modulation of cellular sources of extracellular matrix (ECM), the stimulation of ECM degradation and prevention of collagen crosslinking. Importantly, significant antifibrotic effects have been demonstrated with both IFN-based and IFN-free anti-HCV regimens.Expert opinion: Successful future therapy is likely to be based on sequential administration of drugs leading initially to HCV clearance, followed by treatment for the possible reversal of liver fibrosis. The primary consideration with clinical trials carried out in patients with advanced liver disease is safety. Indeed, the evaluation of anti-fibrotic therapy depends on reliable noninvasive techniques for quantification of liver fibrosis, such as transient or shear-wave elastography or serologic tests which are able to replace liver biopsy. © 2015 © Informa UK, Ltd. Source

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