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Tomczyk M.,Medical University of Biaystok | Tumanov A.,National Academy of Sciences of Belarus | Zaniewska A.,Medical University of Bialystok | Surazynski A.,Medical University of Bialystok
Planta Medica | Year: 2010

The effects of oxidative stress on collagen and DNA biosynthesis, β-galactosidase activity, the expression of the β-integrin receptor, FAK, the insulin-like growth factor-I receptor (IGFIR), the hypoxia-inducible factor-1 (HIF-1), and the mitogen-activated protein kinases (MAP/ERK 1, ERK2) were evaluated in human endometrial carcinoma cells. Subconfluent cells were subjected to oxidative stress with 30μ M t-butylhydroperoxide (t-BHP) for 1h per day over the course of 5 days. It was found that oxidative stress contributed to an increase in the β-galactosidase activity as well as to the inhibition of collagen and DNA biosynthesis. The mechanism of the process was found at the level of IGFIR and HIF-1. An increase in the expression of HIF-1 and a decrease in the expression of IGFIR were observed in the cells subjected to oxidative stress. The role of IGFIR signalling in the process was confirmed by an experiment showing downregulation of MAP kinases ERK1 and ERK2 expression in the studied cells. This phenomenon is probably responsible for the drastic inhibition of protein (up to 40% of control) and DNA biosynthesis (up to 65% of control) in the cells. An addition of tiliroside to the cells medium restored all parameters to the control level, including IGFIR and HIF-1 expressions. The data suggest that the antioxidative activity of tiliroside isolated from Potentilla argentea may originate at the level of IGFIR and HIF-1 signalling. © Georg Thieme Verlag KG Stuttgart - New York.


Krajewska-Kullak E.,Medical University of Biaystok | Kulak W.,Medical University of Bialystok | Marcinkowski J.T.,Poznan University of Medical Sciences | Damme-Ostapowicz K.V.,Medical University of Biaystok | And 4 more authors.
CIN - Computers Informatics Nursing | Year: 2011

The objective of this research was to assess Internet addiction among students of the Faculty of Health Prevention at the Medical University of Białystok. The present study included 358 students-nursing (n = 232), midwifery (n = 71), and medical rescue (n = 55). The following instruments were administered to the participants:the Young test, a test of the intensity of the abstinence syndrome, and a test of "online" addiction. Students who did not have a computer at home spent 3 hours a day on the Internet; students who did have a computer at home spent 0.5 to 8hours. On average, all respondents spent 1.8 ± 1.3 hours daily online. Internet addiction was confirmed among 24 (10.3%) nursing, 7 (9.9%) midwifery, and 5(9.1%) medical rescue students. The abstinence syndrome was noted among 11 (4.7%) nursing, 7(9.9%) obstetrics, and 7 (12.7%) medical rescue students. Several students had both an Internet addiction and the abstinence syndrome. Copyright © 2011 Wolters Kluwer Health | Lippincott Williams & Wilkins.


Gowinska-Olszewska B.,Medical University of Bialystok | Michalak J.,Medical University of Biaystok | Luczynski W.,Medical University of Biaystok | Del Pilar Larosa M.,RSR Limited | And 4 more authors.
Journal of Pediatric Endocrinology and Metabolism | Year: 2016

Background: The aim of this study was to assess the prevalence of diabetes and other organ-specific autoantibodies (Ab) associated with various autoimmune conditions, in Polish children with type 1 diabetes mellitus (T1DM). Methods: In this study 114 patients, aged 13.4 years, with mean diabetes duration 5.2 years were included. Ab to islet cell antigens: glutamic acid decarboxylase (GAD), insulinoma antigen 2 (IA-2), zinc transporter 8 (ZnT8), together with thyroid peroxidase Ab (TPO Ab), thyroglobulin Ab (Tg Ab), tissue transglutaminase Ab (tTG Ab) and 21-hydroxylase Ab (21-OH Ab) were measured. Results: The prevalence of at least one diabetes associated Ab was found in 87%, with the highest prevalence of 64% for ZnT8 Ab. In patients with disease duration < 5 years, at least one antibody was present in 90%, the most prevalent was ZnT8 Ab (72%). In patients with duration > 10 years, 50% had at least one antibody. The prevalence of other than islet cell autoimmunity was high (34%). Thyroid Ab were detected in 26% patients, 42% in girls vs. 8% in boys, p < 0.001. tTG Ab were found in 11% patients, with a greater prevalence in children with early onset (p = 0.01). 21-OH Ab were found in 2.6% T1DM patients. Conclusions: Islet Ab were found in most T1DM children and remained positive even 10 years after onset. ZnT8 Ab emerged as an important marker for the diagnosis of T1DM in the Polish children. Screening for non-diabetes Ab in T1DM may be helpful in identifying subclinical cases of autoimmune thyroid, celiac or Addison's disease (AD).


Jaroszewicz J.,Medical University of Bialystok | Flisiak-Jackiewicz M.,Medical University of Biaystok | Lebensztejn D.,Medical University of Biaystok | Flisiak R.,Medical University of Bialystok
Expert Opinion on Investigational Drugs | Year: 2015

Introduction: More than 100 million people worldwide are infected with hepatitis C virus (HCV), which is responsible for chronic liver disease accompanied by progressive fibrosis of hepatic tissue, often leading to liver cirrhosis. Novel therapeutic options are able to clear the virus in almost all diagnosed patients. However, even after successful treatment, hepatic fibrosis may persist in many of them. There is no registered therapy specific for liver fibrosis, but numerous molecules are currently in development.Areas covered: In this review, the authors look at drugs in early development for the treatment of HCV-related hepatic fibrosis. Their mechanism of action is based on the inhibition of hepatic inflammation, the modulation of cellular sources of extracellular matrix (ECM), the stimulation of ECM degradation and prevention of collagen crosslinking. Importantly, significant antifibrotic effects have been demonstrated with both IFN-based and IFN-free anti-HCV regimens.Expert opinion: Successful future therapy is likely to be based on sequential administration of drugs leading initially to HCV clearance, followed by treatment for the possible reversal of liver fibrosis. The primary consideration with clinical trials carried out in patients with advanced liver disease is safety. Indeed, the evaluation of anti-fibrotic therapy depends on reliable noninvasive techniques for quantification of liver fibrosis, such as transient or shear-wave elastography or serologic tests which are able to replace liver biopsy. © 2015 © Informa UK, Ltd.


Van Damme-Ostapowicz K.,Medical University of Biaystok | Krajewska-Kuak E.,Medical University of Biaystok | Rozwadowska E.,Medical University of Biaystok | Nahorski W.L.,Institute of Tropical Medicine | Olszaski R.,Institute of Tropical Medicine
Malaria Journal | Year: 2012

Background: Health status is one of the basic factors of a high quality of life and the problem of the acceptance of illness is important for adaptation to the limitations imposed by it. The purpose of the study was the evaluation of the quality of life, satisfaction with life and the acceptance of illness by malaria patients, as well as the discovery of a relationship between studied parameters. Methods: The study was undertaken in August 2010, on 120 Nigerian patients with confirmed malaria. A method of diagnostic survey, based on standardized scales - Acceptance of Illness Scale, The Satisfaction With Life Scale and a standardized survey questionnaire World Health Organization Quality of Life/BREF - was used in this study. Descriptive statistics, variability range, 95% confidence interval, correlation analysis, Spearmans non-parametric correlation coefficient, MannWhitney test and Kruskal-Wallis test were applied and the, so called, test statistics was calculated, followed by the calculation of the test probability p. Results of analyses were presented in a box graph, and a graph of dispersion. Results: A dominating share in the adjective scale of the AIS scale was the category of "no acceptance", given by 71.7% of respondents. The average level of a "somatic domain" was 41.7, and of a "social domain" was 62.8. The mean satisfaction of life evaluation in the SWLS scale was 18 points. The correlation between acceptance of the disease and quality of life for the psychological domain was 0.39, and between acceptance of the disease and satisfaction with life was 0.40. The correlation between satisfaction with life and quality of life for the psychological domain was 0.65, and between satisfaction with life and quality of life for the environment domain was 0.60. The mean level of AIS for the studied population of men was 16.5, and test probability: p = 0.0014, and for the environment domain the level was 50, and the test probability: p = 0.0073. For quality of life in the social sphere the test probability: p = 0.0013 in relatively older individuals. Conclusion: The majority of people do not accept their condition. Evaluation of the quality of life was the highest in the social domain, and the lowest in the somatic domain. There is a statistically significant correlation between the level of acceptance of illness and the quality of life and satisfaction with life. The strongest correlation is found between satisfaction with life and the evaluation of the quality of life in psychological and environmental domains. Men evaluate their quality of life in the environmental domain higher and demonstrate a higher acceptance of their disease. There is a correlation regarding a significantly higher quality of life in the social sphere in relatively older people. © 2012 Van Damme-Ostapowicz et al.


Cechowska-Pasko M.,Medical University of Biaystok
Cell biology international | Year: 2011

We decided to study the effect of glucose deprivation on collagen metabolism in MCF7 cells. The incorporation of [3H]-proline into collagenase-sensitive and hydroxyproline-containing proteins was used as an index of collagen synthesis, whereas pulse-chase technique was employed to evaluate the degradation of newly synthesized proteins. The MCF7 cells incubated in high glucose medium synthesized detectable amounts of collagenous proteins. Most of them were found in the cell layer. The shortage of glucose resulted in about 30% reduction in collagen synthesis. The pulse-chase experiments demonstrated that proportionally less collagen was degraded in cultures incubated in low-glucose than in high-glucose media.


Knapp P.,Medical University of Biaystok | Chabowski A.,Medical University of Biaystok | Harasiuk D.,Medical University of Biaystok | Gorski J.,Medical University of Biaystok
Hormone and Metabolic Research | Year: 2012

Cancer cells exhibit accelerated rates of metabolism favoring glucose over fatty acid (FA) utilization. For both energy substrates, protein-mediated transport plays an essential role in facilitating glucose or FA movement across plasma membrane into the cells. Scarce data exist regarding the expression of glucose and/or FA transporter in cancer tissue. Therefore, we examined glucose (GLUT-1, GLUT-3, GLUT-4) and FA (FAT/CD36, FABPpm, FATP-1) transporter expressions at the protein and post-transcript (mRNA) levels in 35 endometrial carcinomas (G1, type endometrioid, FIGO I) and compared them with normal endometrial mucosa (n=10). Endometrial cancer tissue had significantly greater protein expression of GLUT-1, GLUT-3, and GLUT-4 (+ 40%; + 20%; + 24%; p<0.05, respectively) and, conversely, lower fatty acid (FAT/CD36 and FATP-1) transporter expression ( 25%; p<0.05 and 15%, p>0.05 respectively). Interestingly, mRNA content closely mirrors the changes, but only for glucose transporters and not fatty acid transporters. These results suggest the presence of metabolic switch of energy utilization in endometrial cancers favoring glucose consumption as the major source of energy. © Georg Thieme Verlag KG · Stuttgart · New York.


Kuc P.,Medical University of Biaystok | Lemancewicz A.,Medical University of Biaystok | Laudanski P.,Medical University of Biaystok | Kretowska M.,Technical University of Biaystok | Laudanski T.,Medical University of Biaystok
Folia Histochemica et Cytobiologica | Year: 2010

Preterm labour and prematurity are still a main cause of perinatal morbidity nowadays. The aim of our study was to assess the role of MMP-8 as a predictive marker of preterm delivery. Four groups of patients were involved to the study: I - pregnant women at 24-34 weeks of gestation with any symptoms of threatened preterm labour; II - threatened preterm labour patients between 24-34 weeks of gestation; III - preterm vaginal delivery patients; IV - healthy term vaginal delivery patients. Serum concentration of total MMP-8 was measured using two enzyme-linked immunosorbent assays. There were no significant differences in the median concentrations of total MMP-8 between physiological pregnancy and threatened preterm labour patients with existing uterine contractility. No significant differences of total MMP-8 were either found between healthy term and preterm labouring patients. The studies on a larger population are needed to reject the hypothesis that preterm labour is connected with increased MMP-8 plasma concentrations of women in preterm labour and threatened preterm delivery. © Polish Histochemical et Cytochemical Society.


Moreno-Navarrete J.M.,Institute Dinvestigacio Biomedica Of Girona | Blasco G.,Girona Biomedical Research Institute | Xifra G.,Institute Dinvestigacio Biomedica Of Girona | Karczewska-Kupczewska M.,Medical University of Biaystok | And 11 more authors.
Journal of Clinical Endocrinology and Metabolism | Year: 2016

Context: Different geneticandimaging iron markers areknowntobeincreased in the liver, adipose tissue, and brain of obese subjects. Objective: We aimed to investigate these markers in human skeletal muscle. Design, Setting, Patients, and Outcome Measures: Markers of iron accumulation were measured in three different territories: Iron gene markers (TFRC1, TF, SLC11A2, FTL, FTH1, and SLC40A1) were studied in abdominal rectus abdominis (Cohort 1, n=26) and quadriceps (Cohort 2, n=13) muscle using real-time PCR, whereas paravertebral muscle R2∗ signal (as surrogate of iron content) (Cohort 3, n = 43) was evaluated by means of magnetic resonance imaging. Intervention: In a subgroup of 14 obese participants from Cohort 3, a diet-induced weight loss was performed. Results: Rectus abdominis muscle age-adjusted gene expression of SLC40A1 (ferroportin) (r=0.47; P =.04), SLC11A2 (r = 0.50; P =.03) and CYBA (r = 0.62; P =.006) increased with body fatness. In obese participants from Cohort 1, muscle CYBA gene expression was positively correlated with serum ferritin. This association was replicated in quadriceps from obese participants (Cohort 2). Paravertebral muscle R2∗ was positively associated with body mass index, waist circumference, and fat mass (measured by dual-energy X-ray absorptiometry) in parallel with hepatic iron content, serumferritin,andhepcidin. In multivariate regression analyses, obesity parameters (P=.0001)and hsCRP concentration (P =.05) contributed independently to the variance of sex-, serum hepcidinand age-adjusted muscle R2∗. Of note, weight loss intervention resulted in decreased muscle R2∗ (P =.02) in correlation with the change of serum ferritin (r = 0.69; P =.01). Conclusions: These findings emphasize a significant iron accumulation in human skeletal muscle in association with obesity. The mechanisms implicated in these observations should be studied further. © 2016 by the Endocrine Society.


PubMed | Medical University of Biaystok
Type: Journal Article | Journal: Cell biology international | Year: 2011

We decided to study the effect of glucose deprivation on collagen metabolism in MCF7 cells. The incorporation of [3H]-proline into collagenase-sensitive and hydroxyproline-containing proteins was used as an index of collagen synthesis, whereas pulse-chase technique was employed to evaluate the degradation of newly synthesized proteins. The MCF7 cells incubated in high glucose medium synthesized detectable amounts of collagenous proteins. Most of them were found in the cell layer. The shortage of glucose resulted in about 30% reduction in collagen synthesis. The pulse-chase experiments demonstrated that proportionally less collagen was degraded in cultures incubated in low-glucose than in high-glucose media.

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