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Gong Y.-B.,Peking University | Zheng J.-L.,Medical Science Institute of Liaoning | Jin B.,Peking University | Zhuo D.-X.,Peking University | And 7 more authors.
PLoS ONE | Year: 2012

Background: Candida albicans is a human commensal that is also responsible for chronic gastritis and peptic ulcerous disease. Little is known about the genetic profiles of the C. albicans strains in the digestive tract of dyspeptic patients. The aim of this study was to evaluate the prevalence, diversity, and genetic profiles among C. albicans isolates recovered from natural colonization of the digestive tract in the dyspeptic patients. Methods and Findings: Oral swab samples (n = 111) and gastric mucosa samples (n = 102) were obtained from a group of patients who presented dyspeptic symptoms or ulcer complaints. Oral swab samples (n = 162) were also obtained from healthy volunteers. C. albicans isolates were characterized and analyzed by multilocus sequence typing. The prevalence of Candida spp. in the oral samples was not significantly different between the dyspeptic group and the healthy group (36.0%, 40/111 vs. 29.6%, 48/162; P & 0.05). However, there were significant differences between the groups in the distribution of species isolated and the genotypes of the C. albicans isolates. C. albicans was isolated from 97.8% of the Candida-positive subjects in the dyspeptic group, but from only 56.3% in the healthy group (P < 0.001). DST1593 was the dominant C. albicans genotype from the digestive tract of the dyspeptic group (60%, 27/45), but not the healthy group (14.8%, 4/27) (P < 0.001). Conclusions: Our data suggest a possible link between particular C. albicans strain genotypes and the host microenvironment. Positivity for particular C. albicans genotypes could signify susceptibility to dyspepsia. © 2012 Gong et al. Source


Qi H.,Medical Science Institute of Liaoning | Gong Y.-B.,Peking University | Huang Z.-Q.,Medical Science Institute of Liaoning | Liu Y.,Medical Science Institute of Liaoning | And 5 more authors.
World Chinese Journal of Digestology | Year: 2015

AIM: To detect the genetic profiles of Candida albicans (C. albicans) strains in the digestive tract of dyspeptic patients by multilocus sequence typing (MLST), and to explore whether lesion of the gastric mucosa is closely related to the genotype of C. albicans. METHODS: A total of 111 oral swab samples and 102 gastric mucosa samples were collected from patients with gastritis or gastric ulcer. In addition, 162 oral swab samples collected from healthy volunteers were used as a control group. Candida species isolates from separate samples were identified by amplifying the ITS1- 5.8S-ITS2 region sequence. C. albicans isolates were characterized and analyzed by multilocus sequence typing, and submitted to the C. albicans MLST database. The phylogenetic tree was constructed by the method of unweightedpair group method using average linkages (UPGMA) to analyze the relationship between the evolutionary clades and gastric mucosal inflammation lesion. RESULTS: In the oral mucosa swab samples of the control group and patient group, the positive rates of Candida spp. were 29.6% vs 36.0%, and the constitute ratios of C. albicans were 64.6% vs 95%, respectively. In the gastric samples of the patient group, the positive rate of Candida spp. was 41.4%, and the constituent ratio of C. albicans was 97.8%. Both the positive rate and constituent ratio of C. albicans in the patient group were significantly higher than those in the control group (χ2 = 4.071, P < 0.01; χ2 = 7.650, P = 0.006). In C. albicans MLST detection, the positive rate of genotype ST1593 was significantly higher in the patient group than in the control group (60% vs 14.8%; χ2 = 12.815, P < 0.001). The different evolutionary clades of C. albicans strains were closely related to the inflammatory lesion of the gastric mucosa (Kendall’s tau-b r = 0.591, P < 0.001). CONCLUSION: C. albicans could be detected in the gastric mucosa of patients with dyspepsia, and its special genotype is closely related to the inflammatory lesion of the gastric mucosa. © 2015 Baishideng Publishing Group Inc. All rights reserved. Source

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