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To clarify age-related histological and zinc content changes in paediatric and nonhyperplastic young adult prostate glands, a quantitative morphometric and energy-dispersive X-ray fluorescence analysis (EDXRF) was performed. The prostates were obtained at autopsy from 50 subjects (European-Caucasian aged 0-30 years) who died from sudden infant death syndrome, acute pulmonary aetiologies and trauma. None of the subjects had any symptoms of prostatic disease and all prostates were classified as histologically normal. Each prostate was divided into two portions. One tissue portion was reviewed by an anatomical pathologist whereas another was intended for a Zn mass fraction measurement using EDXRF. The mean per cent volume of the stroma (S), glandular epithelium (E), glandular lumen (L) and glandular component (GC = E + L) were determined and the mean ratios of per cent volumes (S/E, S/GC and E/L) were calculated for each prostate specimen. It was found that normal prostate tissue undergoes substantial changes from birth through early adulthood. These changes are reflected in an increment of the per cent volume of the glandular epithelium and lumen and in a diminution of the per cent volume of the stroma. The S/E ratio of the prostate falls by a factor of almost 4, between ages <1 and 30 years from 5 : 1 to 1.3 : 1. The Zn mass fraction is nearly 30-35 mg/kg (wet weight basis) and remains steady for first decade of life. Then it begins to increase rapidly during and after puberty. The level of the Zn content in prostate tissue is most closely associated with the volume of the glandular lumen, which reflects the volume of prostatic fluid. © 2012 American Society of Andrology and European Academy of Andrology. Source


Zaichick V.,Medical Radiological Research Center | Zaichick S.,Northwestern University
Journal of Radioanalytical and Nuclear Chemistry | Year: 2013

The effect of age on trace element contents in intact prostate of 50 apparently healthy 0-30 year old males was investigated by neutron activation analysis with high resolution spectrometry of long-lived radionuclides. Mean values (M ± SΕΜ) for mass fraction (milligram per kilogram, on dry-weight basis) of trace elements were: Ag 0.0708 ± 0.0096, Co 0.0348 ± 0.0040, Cr 0.466 ± 0.069, Fe 100 ± 10, Hg 0.0258 ± 0.0025, Rb 12.6 ± 0.8, Sb 0.0576 ± 0.0066, Sc 0.0125 ± 0.0016, Se 0.478 ± 0.031, and Zn 273 ± 31, respectively. A strongly pronounced tendency of age-related increase in Se and Zn mass fraction and of age-related decrease in Co, Cr, Fe, and Sc mass fraction was observed in period of life from 0 to 30 years. © 2013 Akadémiai Kiadó, Budapest, Hungary. Source


Zaichick S.,Northwestern University | Zaichick V.,Medical Radiological Research Center
X-Ray Spectrometry | Year: 2011

A method for radionuclide-induced (109Cd) energy-dispersive X-ray fluorescent (EDXRF) was used to determine Br, Fe, Rb, Sr, and Zn contents in intact and morphologic normal prostate tissues. Prostates were removed at necropsy from 64 men (mean age 36.5years, range 13-60) who had died suddenly. All materials were divided into two parts. One part was morphologically examined, whereas chemical element contents of the other were estimated. Mean values (M±SΕΜ) for mass fraction of Br, Fe, Rb, Sr, and Zn (mgkg-1 on dry-weight basis) in the intact and morphologic normal prostate tissue were 35.5±4.0, 107±5.4, 17.1±0.8, 1.9±0.3, and 850±79, respectively. Mean values (M±SΕΜ) for Zn/Fe, Zn/Rb, and Zn/Sr ratios of mass fraction were 8.72±0.80, 57.4±7.2, and 567±82, respectively. A strongly pronounced tendency of age-related exponential increase in Zn mass fraction as well an increase in Zn/Fe, Zn/Rb, and Zn/Sr ratios in prostate was observed. A significant positive correlation was seen between the prostatic zinc and iron contents (p≤0.05, r=0.22) and between the prostatic zinc and bromine contents (p≤0.01, r=0.43). © 2011 John Wiley & Sons, Ltd. Source


Zaichick V.,Medical Radiological Research Center | Zaichick S.,Northwestern University
Applied Radiation and Isotopes | Year: 2013

The effect of age on chemical element mass fractions in intact prostate of 50 apparently healthy 0-30 year old males was investigated by neutron activation analysis with high resolution spectrometry of short-lived radionuclides. Mean values (M±SΕΜ) for mass fraction (mgkg-1, dry mass basis) of chemical elements before the time of puberty and in the period of puberty and post-puberty were: Br 46.0±6.7, Ca 1151±140, Cl 14572±700, K 10147±700, Mg 771±131, Mn 2.13±0.25, Na 9880±659 and Br 29.0±4.6, Ca 2049±364, Cl 11518±1121, K 13029±542, Mg 1186±134, Mn 1.74±0.16, Na 9887±716, respectively. A tendency of age-related increase in Ca, K, and Mg mass fraction and of age-related decrease in Br mass fraction was observed in period of life from 0 to 30 years. This new data indicates that of the elements studied, only the Ca, K, and Mg mass fraction in prostate tissue is an androgen-dependent parameter. © 2013 Elsevier Ltd. Source


Zaichick V.,Medical Radiological Research Center
Medical Hypotheses | Year: 2014

Several experiments show that prostatic fluid is continuously produced and it is drained from the prostate during urination and ejaculation. The mechanism which causes prostatic fluid to drain from the prostatic acini during urination is currently unclear. Also in current opinion such structures of the prostatic urethra as the urethral crest and the colliculus seminalis have no apparent functional significance. This article describes a mechanism for the draining of the prostatic acini that involves these prostatic urethral structures. It is hypothesized that the prostatic urethra works as a pump using the Venturi effect, in which urine is the carrying or motive liquid during voiding, in order to drain prostatic fluid (the carried liquid) from the acini. The urethral crest and the colliculus seminalis take part in controlling flow rates and liquid pressures for this pump to be effective. The calculated estimation of a pressure drop in the region of the colliculus seminalis during micturition was obtained using morphometric and uroflowmetric data and was used to confirm this hypothesis of prostatic acini drainage. As a consequence of this, a previously unknown function for these intra-prostatic urethral structures is described. © 2014 Elsevier Ltd. Source

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