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Hadizadeh M.,Iranian Research Organization for Science and Technology | Fateh M.,Medical Laser Research Center
Iranian Journal of Medical Sciences | Year: 2014

Background: Photodynamic therapy (PDT) is a promising therapeutic modality for the treatment of cancer and other diseases. In this study, the epidermoid carcinoma cell line A431 and the normal fibroblasts were used to investigate whether gold nanoparticles (GNPs) can induce an increase in cell death during PDT using 5-aminolevulinic acid (5-ALA) as a photosensitizer.Methods: Human fibroblast and A431 cells were grown in 96-well plates. The effect of GNPs on the efficacy of 5-ALAmediated PDT (5-ALA-PDT) was evaluated by comparing the effect of 5-ALA with GNPs to the effect of 5-ALA alone. Cell viability was determined by the methyl- tetrazolium assay.Results: Dark toxicity experiments showed that 5-ALA at concentrations 0.5, 1 and 2 mM had no significant effect on cell viability of both cell lines. However, treatment of cells with 5-ALA (0.5 to 2 mM) and light dose of 25 Jcm-2 led to 5-10% and 31-42% decrease in cell viability of fibroblast and A431 cells respectively. The data also shows that GNPs in both the absence and the presence of light, results in a dose-dependent decrease in cell viability of both cell lines. However, the sensitivity of cancer cells to GNPs at concentrations more than 24 μg/ml was approximately 2.5- to 4-fold greater than healthy cells. Furthermore, data indicates that 5-ALA in combination with GNPs results in a synergistic reduction in viability of A431 cells.Conclusion: In summary, the findings of this study suggest that concomitant treatment with 5-ALA and GNPs may be useful in enhancing the effect of 5-ALA-PDT. © 2014, Shiraz University of Medical Sciences. All rights reserved.


PubMed | Iran National Institute of Genetic Engineering and Biotechnology, Medical Laser Research Center and Iran University of Science and Technology
Type: | Journal: Iranian biomedical journal | Year: 2017

Improved cyan fluorescent protein (ICFP) is a monochromic, green fluorescent protein (GFP) derivative produced by Aequorea macrodactyla in a process similar to GFP. This protein has strong absorption spectra at wavelengths 426-446 nm. ICFP can be used in cell, organelle or intracellular protein labeling, investigating the protein-protein interactions as well as assessing the promoter activities.In our previous study, the promoters of two chitinases (ChiS and ChiL) from Bacillus pumilus SG2 were assessed in B. subtilis and their regulatory elements were characterized. In the present study, icfp was cloned downstream of several truncated promoters obtained in the former study, and ICFP expression was evaluated in B. subtilis.Extracellular expression and secretion of ICFP were analyzed under the control of different truncated versions of ChiSL promoters grown on different media. Results from SDS-PAGE and fluorimetric analyses showed that there were different expression rates of CFP; however, the UPChi-ICFP3 construct exhibited a higher level of expression and secretion in the culture medium.Our presented results revealed that inserting this truncated form of Chi promoter upstream of the ICFP, as a reporter gene, in B. subtilis led to an approximately ten fold increase in ICFP expression.


PubMed | Golestan University of Medical Sciences, Medical Laser Research Center, Tehran University of Medical Sciences and Guilan University
Type: Journal Article | Journal: The Korean journal of pain | Year: 2016

This study aimed to investigate the effect of strenuous exercise on -endorphine (-END) level in fibromyalgia (FM) patients compared to healthy subjects.We enrolled 30 FM patients and 15 healthy individuals. All study participants underwent a treadmill exercise test using modified Bruce protocol (M.Bruce). The goal of the test was achieving at least 70% of the predicted maximal heart rate (HRMax). The serum levels of -END were measured before and after the exercise program. Measurements were done while heart rate was at least 70% of its predicted maximum.The mean the standard deviation (SD) of exercise duration in the FM and control groups were 24.26 5.29 and 29.06 3.26 minutes, respectively, indicating a shorter time to achieve the goal heart rate in FM patients (We found that FM patients had lower levels of -END in both basal and post-exercise status. Exercise increased serum the -END level in both groups but the average increase in -END in FM patients was significantly lower than in the control group.


Hosseinzadeh R.,Medical Laser Research Center | Khorsandi K.,Medical Laser Research Center | Sheikh-Hasani V.,University of Tehran | Khatibi A.,University of Tehran
Journal of Biomolecular Structure and Dynamics | Year: 2016

The binding of thiamine (vitamin B1) on lysozyme has been examined at various ionic strengths of phosphate buffer (pH 6.9), various pH values, and various protein concentrations at 25°C using thiamine selective membrane electrode. This method is faster and more precise than equilibrium dialysis technique which can obtain sufficient and accurate data for binding analysis. The values of Hill equation parameters were estimated for each set using binding capacity concept and used for calculation of intrinsic binding affinity. The results represent two binding sets for thiamine on lysozyme at various experimental conditions. © 2016 Taylor & Francis


Hosseinzadeh R.,University of Tehran | Hosseinzadeh R.,Medical Laser Research Center | Moosavi-Movahedi A.A.,University of Tehran
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy | Year: 2016

Here, the effect of benzene on hemoglobin structure, stability and heme prosthetic group integrity was studied by different methods. These included UV-vis absorption spectrophotometry, normal and synchronous fluorescence techniques, and differential scanning calorimetry (DSC). Our results indicated that benzene has high hemolytic potential even at low concentrations. The UV-vis spectroscopic results demonstrated that benzene altered both the globin chain and the heme prosthetic group of hemoglobin increasing met- and deoxy-Hb, while decreasing oxy-Hb. However, with increasing benzene the concentration of all species decreased due to heme destruction. The spectrophotometric results show that benzene has a high potential for penetrating the hydrophobic pocket of hemoglobin. These results were consistent with the molecular docking simulation results of benzene-hHb. Aggregation and thermal denaturation studies show that the increased benzene concentration induced hemoglobin aggregation with a decrease in stability, which is consistent with the DSC results. Conventional fluorescence spectroscopy revealed that the heme degradation species were produced in the presence of benzene. The results of constant wavelength synchronous fluorescence spectroscopy (CWSFS) indicated that at least five heme-degraded species were produced. Together, our results indicated that benzene has adverse effects on hemoglobin structure and function, and heme degradation. © 2015 Elsevier B.V. All rights reserved.


PubMed | Karolinska Institutet, Copenhagen University, Medical Laser Research Center, Nuclear Science and Technology Research Institute, Iran and 2 more.
Type: | Journal: Colloids and surfaces. B, Biointerfaces | Year: 2016

Favorable physiochemical properties and the capability to accommodate targeting moieties make superparamegnetic iron oxide nanoparticles (SPIONs) popular theranostic agents. In this study, we engineered SPIONs for magnetic resonance imaging (MRI) and photothermal therapy of colon cancer cells. SPIONs were synthesized by microemulsion method and were then coated with gold to reduce their cytotoxicity and to confer photothermal capabilities. Subsequently, the NPs were conjugated with thiol modified MUC-1 aptamers. The resulting NPs were spherical, monodisperse and about 19nm in size, as shown by differential light scattering (DLS) and transmission electron microscopy (TEM). UV and X-ray photoelectron spectroscopy (XPS) confirmed the successful gold coating. MTT results showed that Au@SPIONs have insignificant cytotoxicity at the concentration range of 10-100g/ml (P>0.05) and that NPs covered with protein corona exerted lower cytotoxicity than bare NPs. Furthermore, confocal microscopy confirmed the higher uptake of aptamer-Au@SPIONs in comparison with non-targeted SPIONs. MR imaging revealed that SPIONs produced significant contrast enhancement in vitro and they could be exploited as contrast agents. Finally, cells treated with aptamer-Au@SPIONs exhibited a higher death rate compared to control cells upon exposure to near infrared light (NIR). In conclusion, MUC1-aptamer targeted Au@SPIONs could serve as promising theranostic agents for simultaneous MR imaging and photothermal therapy of cancer cells.


Bidari A.,Tehran University of Medical Sciences | Hassanzadeh M.,Tehran University of Medical Sciences | Mohabat M.-F.,Medical Laser Research Center | Talachian E.,Tehran University of Medical Sciences | Khoei E.M.,Tehran University of Medical Sciences
Rheumatology International | Year: 2014

The aim of this study is to translate, adapt, and validate a Persian version of the Fibromyalgia (FM) Impact Questionnaire (FIQ-P). The FIQ-P was adapted following the translation and back-translation approach; then, it was administered to thirty females with FM. Participants also completed two other validated questionnaires, the Medical Outcome Survey Short Form-36 (SF-36) and the Beck Depression Inventory (BDI). Internal consistency within the FIQ-P items and its test-retest reliability were assessed with Cronbach's alpha and Spearman's correlation coefficient, respectively. Construct validity was analyzed by Spearman's r when correlating the FIQ-P to other questionnaires. The translated version was concordant. Adaptation affected two sub-items of physical function. Participants' mean age ± standard deviation was 40.4 ± 9.0 years. Internal consistency proved good with α = 0.80. Test-retest coefficient ranged from 0.50 for the item "work days missed" to 0.79 for all FIQ-P items. Fair and statistically significant (P < 0.01) correlations were found between the FIQ-P items and two other questionnaires, SF-36 (r = -0.57) and BDI (r = 0.53). We concluded that the FIQ-P is a valid and reliable instrument for measuring health status of Persian-speaking FM patients. © 2013 Springer-Verlag Berlin Heidelberg.


Hosseinzadeh R.,Medical Laser Research Center | Khorsandi K.,Medical Laser Research Center
Journal of Biomolecular Structure and Dynamics | Year: 2016

Vitamin B1 or thiamin is one of the B vitamins. All B vitamins help the body to convert food (carbohydrates) into fuel (glucose), which produces energy. The B vitamins are necessary for healthy skin, eyes, hair, and liver. It also could help the nervous system function properly, and is necessary for brain functions. Drug interactions with protein can affect the distribution of the drug and eliminate the drug in living systems. In this study, the binding of thiamine hydrochloride (vitamin B1) to bovine serum albumin (BSA) was evaluated using a new proposed vitamin B1 (thiamine)-selective membrane electrode under various experimental conditions, such as pH, ionic strength, and protein concentration; in addition molecular modeling was applied as well. The binding isotherms plotted based on potentiometric data and analyzed using the Wyman binding potential concept. The apparent binding constant was determined and used for the calculation of intrinsic Gibbs free energy of binding. According to the electrochemical and molecular docking results, it can be concluded that the hydrophobic interactions and hydrogen binding are major interactions between BSA and vitamin B1. 2015 © 2016 Informa UK Limited, trading as Taylor & Francis Group


Khorsandi K.,Medical Laser Research Center | Hosseinzadeh R.,Medical Laser Research Center | Fateh M.,Medical Laser Research Center
RSC Advances | Year: 2015

Curcumin, a naturally occurring phenolic compound, is a highly potent anticancer agent against many different types of cancers. Recent studies show that curcumin can be used as a photosensitizer in photodynamic therapy for cancer treatment. However, the major disadvantage of curcumin is its poor aqueous solubility. To improve its applicability in cancer therapy, we intercalated curcumin into layered double hydroxide (LDH) with the co-precipitation method and used as a nanohybrid photosensitizer in photodynamic therapy of human breast cancer cells. Powder X-ray diffraction (XRD), TEM and SEM microscopy analyses indicate that curcumin is stabilized in the host interlayer. According to the spectroscopy results, the water solubility and dispersity of intercalated curcumin increased and loading amount of curcumin in LDH is about 50%. The photodynamic effect of curcumin and the curcumin-LDH nanohybrid was studied on the MDA-MB-123 human breast cancer cell line. Optimization of incubation time with free curcumin and curcumin-LDH nanohybrid as the most effective parameter was investigated. The optimum irradiation time of blue LED on photodynamic therapy was determined for both free curcumin and curcumin-LDH nanohybrid. Cell viability studies revealed that the nanohybrid curcumin-LDH were able to show more effective photodynamic effects on the cancer cells as compared to free curcumin. These results suggest that the biocompatible layered double hydroxide can be used as the basis of a tunable curcumin delivery carrier for photodynamic therapy in breast cancer treatment. © The Royal Society of Chemistry.


PubMed | Tehran University of Medical Sciences and Medical Laser Research Center
Type: Journal Article | Journal: Journal of lasers in medical sciences | Year: 2016

Photobiostimulation with low level laser (LLL) has been used in medicine for a long time and its effects have been shown in many diseases. Some studies have evaluated the effect of LLL on androgenic alopecia. One of the most important limitations of the use of LLL in the treatment of alopecia is the requirement for multiple sessions, which is hardly accepted by patients. This study was conducted to evaluate the effect of the irradiation of extracted follicular hair units by LLL on the outcome of hair transplantation.We enrolled 10 patients with androgenic alopecia and after screening tests for infections and other diseases, we extracted hair follicular units. The hair units were divided in two groups. One group was irradiated by LLL 20 minutes before transplantation (660 nm, 80 Hz, 100 mW) and the other one was used as control. The containing plates were labeled as A and B and sent to the operation room. The surgeon was unaware of the therapy assigned to the plates and transplanted them randomly on the right or left side of the head. One hundred follicular units on each sides of the scalp were transplanted symmetrically. The follicles on both sides were evaluated at 3 and 6 months of transplantation for hair growth rate by another physician, blinded to the treatment assigned to each side.Ten patient with androgenic alopecia and mean (SD) age of 31.5 (6.6) years (range 25-45 years) completed the study. All patients had 100% hair growth at 3 and 6 months follow-up except one who had hair growth of 20% at three months of transplantation, which changed to 100% at sixth months. There was no significant difference between the groups regarding hair growth (P > 0.8).One session of LLL irradiation has no significant effect on the outcome of transplanted hair follicles. Studies with larger sample size are needed to draw a definite conclusion.

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