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Bidari A.,Tehran University of Medical Sciences | Hassanzadeh M.,Tehran University of Medical Sciences | Mohabat M.-F.,Medical Laser Research Center | Talachian E.,Tehran University of Medical Sciences | Khoei E.M.,Tehran University of Medical Sciences
Rheumatology International

The aim of this study is to translate, adapt, and validate a Persian version of the Fibromyalgia (FM) Impact Questionnaire (FIQ-P). The FIQ-P was adapted following the translation and back-translation approach; then, it was administered to thirty females with FM. Participants also completed two other validated questionnaires, the Medical Outcome Survey Short Form-36 (SF-36) and the Beck Depression Inventory (BDI). Internal consistency within the FIQ-P items and its test-retest reliability were assessed with Cronbach's alpha and Spearman's correlation coefficient, respectively. Construct validity was analyzed by Spearman's r when correlating the FIQ-P to other questionnaires. The translated version was concordant. Adaptation affected two sub-items of physical function. Participants' mean age ± standard deviation was 40.4 ± 9.0 years. Internal consistency proved good with α = 0.80. Test-retest coefficient ranged from 0.50 for the item "work days missed" to 0.79 for all FIQ-P items. Fair and statistically significant (P < 0.01) correlations were found between the FIQ-P items and two other questionnaires, SF-36 (r = -0.57) and BDI (r = 0.53). We concluded that the FIQ-P is a valid and reliable instrument for measuring health status of Persian-speaking FM patients. © 2013 Springer-Verlag Berlin Heidelberg. Source

Hosseinzadeh R.,University of Tehran | Hosseinzadeh R.,Medical Laser Research Center | Moosavi-Movahedi A.A.,University of Tehran
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

Here, the effect of benzene on hemoglobin structure, stability and heme prosthetic group integrity was studied by different methods. These included UV-vis absorption spectrophotometry, normal and synchronous fluorescence techniques, and differential scanning calorimetry (DSC). Our results indicated that benzene has high hemolytic potential even at low concentrations. The UV-vis spectroscopic results demonstrated that benzene altered both the globin chain and the heme prosthetic group of hemoglobin increasing met- and deoxy-Hb, while decreasing oxy-Hb. However, with increasing benzene the concentration of all species decreased due to heme destruction. The spectrophotometric results show that benzene has a high potential for penetrating the hydrophobic pocket of hemoglobin. These results were consistent with the molecular docking simulation results of benzene-hHb. Aggregation and thermal denaturation studies show that the increased benzene concentration induced hemoglobin aggregation with a decrease in stability, which is consistent with the DSC results. Conventional fluorescence spectroscopy revealed that the heme degradation species were produced in the presence of benzene. The results of constant wavelength synchronous fluorescence spectroscopy (CWSFS) indicated that at least five heme-degraded species were produced. Together, our results indicated that benzene has adverse effects on hemoglobin structure and function, and heme degradation. © 2015 Elsevier B.V. All rights reserved. Source

Hadizadeh M.,Iranian Research Organization for Science and Technology | Fateh M.,Medical Laser Research Center
Iranian Journal of Medical Sciences

Background: Photodynamic therapy (PDT) is a promising therapeutic modality for the treatment of cancer and other diseases. In this study, the epidermoid carcinoma cell line A431 and the normal fibroblasts were used to investigate whether gold nanoparticles (GNPs) can induce an increase in cell death during PDT using 5-aminolevulinic acid (5-ALA) as a photosensitizer.Methods: Human fibroblast and A431 cells were grown in 96-well plates. The effect of GNPs on the efficacy of 5-ALAmediated PDT (5-ALA-PDT) was evaluated by comparing the effect of 5-ALA with GNPs to the effect of 5-ALA alone. Cell viability was determined by the methyl- tetrazolium assay.Results: Dark toxicity experiments showed that 5-ALA at concentrations 0.5, 1 and 2 mM had no significant effect on cell viability of both cell lines. However, treatment of cells with 5-ALA (0.5 to 2 mM) and light dose of 25 Jcm-2 led to 5-10% and 31-42% decrease in cell viability of fibroblast and A431 cells respectively. The data also shows that GNPs in both the absence and the presence of light, results in a dose-dependent decrease in cell viability of both cell lines. However, the sensitivity of cancer cells to GNPs at concentrations more than 24 μg/ml was approximately 2.5- to 4-fold greater than healthy cells. Furthermore, data indicates that 5-ALA in combination with GNPs results in a synergistic reduction in viability of A431 cells.Conclusion: In summary, the findings of this study suggest that concomitant treatment with 5-ALA and GNPs may be useful in enhancing the effect of 5-ALA-PDT. © 2014, Shiraz University of Medical Sciences. All rights reserved. Source

Tabbodi M.,Iranian Research Organization for Science and Technology | Hadizadeh M.,Iranian Research Organization for Science and Technology | Jahanshiri-Moghadam M.,Medical Laser Research Center
Journal of Zanjan University of Medical Sciences and Health Services

Background and Objective: Photodynamic therapy is a new therapeutic modality for the treatment of cancer. Photodynamic therapy uses an inactive drug and a light source to activate the drug to produce reactive oxygen species that destroy the cancer cells. In the present study, the effect of two different laser light sources on the efficiency of photodynamic therapy was evaluated using a breast cancer cell line. Materials and Methods: MDA-MB-231cells were grown on 96-well plates. After the treatment with different concentrations of 5-aminolevulinic acid, cells were irradiated with a light dose of 10 and/ or 20 Jcm-2 by two different laser light sources. Cell viability was measured by MTT assay. Results: Results showed that 5-aminolevulinic acid at concentrations of 0.5, 1 and 2 mM had no significant cytotoxic effect on the breast cancer cells in the absence of laser activation. However, the treatment of cells with 5-aminolevulinic acid and light dose of 20 Jcm-2 using Lasotronic laser and Mustang laser, decreased cell viability of the breast cancer cells to 80% and 65%, respectively. Conclusion: Light source used in photodynamic therapy plays an important role in the efficiency of this therapeutic modality and light activation of 5-aminolevulinic acid at a photosensitizing concentration using a proper laser light source may potentially be lethal for breast cancer cells. © 2015, Zanjan University of Medical Sciences and Health Services. Source

Tabaie S.M.,Medical Laser Research Center | Ardestani H.B.,Medical Laser Research Center | Azizjalali M.H.,Tehran University of Medical Sciences
Journal of Lasers in Medical Sciences

Introduction: Photobiostimulation with low level laser (LLL) has been used in medicine for a long time and its effects have been shown in many diseases. Some studies have evaluated the effect of LLL on androgenic alopecia. One of the most important limitations of the use of LLL in the treatment of alopecia is the requirement for multiple sessions, which is hardly accepted by patients. This study was conducted to evaluate the effect of the irradiation of extracted follicular hair units by LLL on the outcome of hair transplantation. Methods: We enrolled 10 patients with androgenic alopecia and after screening tests for infections and other diseases, we extracted hair follicular units. The hair units were divided in two groups. One group was irradiated by LLL 20 minutes before transplantation (660 nm, 80 Hz, 100 mW) and the other one was used as control. The containing plates were labeled as A and B and sent to the operation room. The surgeon was unaware of the therapy assigned to the plates and transplanted them randomly on the right or left side of the head. One hundred follicular units on each sides of the scalp were transplanted symmetrically. The follicles on both sides were evaluated at 3 and 6 months of transplantation for hair growth rate by another physician, blinded to the treatment assigned to each side. Results: Ten patient with androgenic alopecia and mean (SD) age of 31.5 (6.6) years (range 25-45 years) completed the study. All patients had 100% hair growth at 3 and 6 months follow-up except one who had hair growth of 20% at three months of transplantation, which changed to 100% at sixth months. There was no significant difference between the groups regarding hair growth (P > 0.8). Conclusion: One session of LLL irradiation has no significant effect on the outcome of transplanted hair follicles. Studies with larger sample size are needed to draw a definite conclusion. Source

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