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Moghbeli M.,Mashhad University of Medical Sciences | Moaven O.,Harvard University | Memar B.,Omeed Hospital | Raziei H.R.,Mashhad University of Medical Sciences | And 7 more authors.
Journal of Gastrointestinal Cancer

Introduction: Gastric cancer (GC) is one of the leading causes of cancer-related death in Iran. Genome stability is one of the main genetic issues in cancer biology which is governed via the different repair systems such as DNA mismatch repair (MMR). A clear correlation between MMR defects and tumor progression has been shown. Beside the genetic mutations, epigenetic changes also have a noticeable role in MMR defects. Methods: Here, we assessed promoter methylation status and the level of hMLH1mRNA expression as the main component of MMR system in 51 GC patients using the methylation-specific PCR and real-time PCR, respectively. Moreover, we performed a promoter methylation study of the E-cadherin gene promoter. Results: It was observed that, 12 out of 39 cases (23.5 %) had hMLH1 overexpression. Hypermethylation of hMLH1 and E-cadherin promoter regions were observed in 25.5 and 36.4 %, respectively. Although, there was no significant correlation between hMLH1 mRNA expression and clinicopathological features, there are significant correlations between E-cadherin promoter methylation and tumor stage (p=0.028) and location (p=0.025). The rate of hMLH1 promoter methylation in this study was lower than that in the other population, showing the importance of the other mechanisms, in gastric tumorigenesis. Conclusion: The results of this study indicate that DNA repair system is adversely affected by hypermethylation of hMLH1 in a fraction of gastric cancer patients. Additionally, E-cadherin hypermethylation seen in a subset of our gastric cancer patients is consistent with other reports showing correlation with aggressiveness and metastasis of gastric cancer. © 2013 Springer Science+Business Media. Source

Manschreck T.C.,Beth Israel Deaconess Medical Center | Chun J.,Beth Israel Deaconess Medical Center | Merrill A.M.,Beth Israel Deaconess Medical Center | Maher B.A.,Beth Israel Deaconess Medical Center | And 7 more authors.
Schizophrenia Research

Background: The Harvard Adolescent Family High Risk (FHR) Study examined multiple domains of function in young relatives of individuals diagnosed with schizophrenia to identify precursors of the illness. One such area is motor performance, which is deviant in people with schizophrenia and in children at risk for schizophrenia, usually offspring. The present study assessed accuracy of motor performance and degree of lateralization in FHR adolescents and young adults. Methods: Subjects were 33 non-psychotic, first-degree relatives of individuals diagnosed with schizophrenia, and 30 non-psychotic comparison subjects (NpC), ranging in age from 13 to 25 who were compared using a line-drawing task. Results: FHR individuals exhibited less precise and coordinated line drawing but greater degree of lateralization than controls. Performance on the linedrawing task was correlated with degree of genetic loading, a possible predictor of higher risk for schizophrenia in the pedigree. Conclusions: The observation of increased motor deviance and increased lateralization in FHR can be utilized in identification and initiation of the treatment in those at high risk in order to prevent or delay the full manifestation of this devastating condition. The use of a rigorously quantified measure is likely to add to the sensitivity of measuring motor performance, especially when impairments may be subtle. © 2015 Elsevier B.V. Source

Alexeeva I.S.,Central Research Institute of Dental and Maxillofacial Surgery | Volkov A.V.,Central Research Institute of Dental and Maxillofacial Surgery | Kulakov A.A.,Central Research Institute of Dental and Maxillofacial Surgery | Goldshtein D.V.,Medical Genetics Research Center
Cellular Transplantation and Tissue Engineering

The clinical and experimental study on the restoration of bone tissue with tissue engineering scaffold based on stem cells of adipose tissue and resorbable osteoplastic matrix. In a pilot study proved the effectiveness of the transplant, founded the dates of dental implantation. Use of tissue engineering scaffold led to organotypic reconstruction of bone tissue in the field of transplantation in patients with severe atrophy of the alveolar process of the maxilla and mandible. As part of a clinical trial were treated 20 patients. Source

Mglinets V.A.,Medical Genetics Research Center
Russian Journal of Genetics

The paper discusses the current data on the genetics of the lens development. Genetically based processes of the formation of the lens anlage, as well as its specification and differentiation, are considered. The main genes responsible for these consecutive processes of lens development are presented. Their mutational disorders can lead to the absence or underdevelopment of the lens or multiple types of cataracts. © 2015, Pleiades Publishing, Inc. Source

Nukuzuma S.,Kobe Institute of Health | Sugiura S.,Medical Genetics Research Center | Nakamichi K.,Japan National Institute of Infectious Diseases | Kameoka M.,Kobe University | And 3 more authors.
Microbiology and Immunology

It has been difficult to study JCV replication because of its restricted host range. In this study, JCV replication was examined using different clones in 293cells. RT-PCR assay revealed that large T antigen expression in cells transfected with IMR-32-adapted JCVs was significantly greater than in those transfected with Mad-1 or CY. DNA replication assay and viral load verified that the IMR-32-adapted JCVs were replication-competent in 293cells, but not Mad-1 or CY JCVs. These results suggest that a 293 culture system with IMR-32-adapted JCVs may be a useful tool for assessing replication of JCV in vitro. © 2015 The Societies and Wiley Publishing Asia Pty Ltd. Source

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