Mahdieh Medical Diagnostic Laboratory

Eşfahān, Iran

Mahdieh Medical Diagnostic Laboratory

Eşfahān, Iran
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Atyabi S.R.,University of Isfahan | Bouzari M.,University of Isfahan | Kardi M.T.,Mahdieh Medical Diagnostic Laboratory
Journal of Medical Virology | Year: 2017

In healthy individuals John Cunningham virus is latent without any clinical signs, but in the cases of the use of immunosuppressive drugs in graft recipients, autoimmune diseases and also increasing of age, that the immune system is suppressed it may cause disease in reactivation. Progressive multifocal leukoencephalopathy (PML) is the well-known disease caused by the virus. It has also been associated with nephropathy and tumorogensis. At present, based on vp1 capsid gene 7 genotypes have been detected. Genetic variations of JC virus in different geographical areas and the presence of different subtypes is a useful tool for reconstructing of the genetic information of JC virus and understanding of its evolution. The aim of this study was to investigate different genotypes of the JC virus in the urine of 100 kidney transplant recipients, 43 rheumatoid arthritis patients, and 100 healthy individuals as control group in Isfahan. DNA was extracted by phenol-chloroform method and subjected to a nested PCR using specific primer for vp1 capsid gene designed by Oligo 7 software. Fisher's exact test was used for statistical analyses. Using MEGA 6 software the sequences were aligned using Clustal W tool and phylogenetic trees were constructed by neighbor joining method. Thirty-one positive samples were sequenced. Genotypes 1, 3, and 4 of the virus were detected for the first time in Iran. For the first time genotype 3 was reported as the dominant genotype in Iran. For the first time in the world, genotype 4 was detected in rheumatoid arthritis patients. J. Med. Virol. 89:337–344, 2017. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.


Ghazimorad A.,University of Isfahan | Bouzari M.,University of Isfahan | Kardi M.T.,Mahdieh Medical Diagnostic Laboratory
Iranian Journal of Blood and Cancer | Year: 2014

Background: Recently, some new viruses have been identified for their association with hepatitis which Torque Teno Mini Virus being among them. The aim of this study was to determine the frequency of Torque Teno Mini Virus in healthy individuals and hepatitis B and C patients in Isfahan, Iran. Materials and Methods: One hundred serum samples of healthy individuals from Isfahan Blood Transfusion Organization were collected. A total of 25 human serum samples from hepatitis B and 25 samples from hepatitis C infected patients were also collected from Mahdieh diagnostic laboratory in Isfahan, Iran. Viral DNA was extracted and Torque Teno Mini Virus DNA was detected using a nested PCR with primer sets designed for a conserved region of the Torque Teno Mini Virus genome. PCR and Reverse transcriptase PCR were used for detection of HBV and HCV respectively. Results: Torque Teno Mini Virus -DNA was detected in 17% of healthy individuals. It also was detected in 20% and 48% of serum samples from hepatitis B and C infected individuals, respectively. The frequency of Torque Teno Mini Virus was significantly higher in hepatitis C patients versus healthy individuals (P < 0.05). Also, the frequency of TTMV in hepatitis C patients was significantly higher than hepatitis B patients (P < 0.05). Conclusions: The difference in Torque Teno Mini Virus frequency between the hepatitis C and healthy group was significant (P< 0.05). The etiology of the higher infection rate in hepatitis C individuals needs to be determined.


Pazhouhnia S.,University of Isfahan | Bouzari M.,University of Isfahan | Rahimi F.,University of Isfahan | Kardi M.T.,Mahdieh Medical Diagnostic Laboratory
Iranian Journal of Blood and Cancer | Year: 2014

Background: Approximately 600,000 deaths occur every year as a result of the acute and chronic consequences of hepatitis B virus infection. Ten different hepatitis B virus genotypes have been identified with distinct geographical distributions. Different clinical outcomes, including the rate of mutations, development of hepatocellular carcinoma, chronicity, response to treatment, transplantation rejection and occult infections, are affected by specific genotypes. The aim of the present study was to determine the frequency of genotype D of the virus in Isfahan, Iran. Patients and Methods: In this study primarily hepatitis B virus positive patients were identified by the detection of HBs antigen using ELISA test and then PCR was used as a confirmatory test. Fifty five patients that were identified as hepatitis B positive were tested for hepatitis D genotype using type - specific PCR. Results: The patients included 30 (54.5%) females and 25 (45.5%) males. In total, frequency of genotype D was 29 out of 55 cases (52.7%). Genotype D was detected in 19 (63.3%) females and 10 (40.0%) males indicating no statistically significant difference. The difference in the level of liver enzymes in patients infected with genotype D and non-genotype D hepatitis B virus were not significant. Conclusion: In the present study the frequency of genotype D among patients with hepatitis B virus infection in Isfahan, Iran, was 52.7%. No significant relation was observed between the level of liver enzymes and infection with the genotype D. © 2014, Iranian Pediatric Hematology and Oncology Society. All rights reserved.

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