Medical Biochemistry and Molecular Biology

Bad Homburg vor der Höhe, Germany

Medical Biochemistry and Molecular Biology

Bad Homburg vor der Höhe, Germany
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Lang S.,Medical Biochemistry and Molecular Biology | Benedix J.,Medical Biochemistry and Molecular Biology | Fedeles S.V.,Yale University | Schorr S.,Medical Biochemistry and Molecular Biology | And 13 more authors.
Journal of Cell Science | Year: 2012

Co-translational transport of polypeptides into the endoplasmic reticulum (ER) involves the Sec61 channel and additional components such as the ER lumenal Hsp70 BiP and its membrane-resident co-chaperone Sec63p in yeast. We investigated whether silencing the SEC61A1 gene in human cells affects co- and post-translational transport of presecretory proteins into the ER and post-translational membrane integration of tail-anchored proteins. Although silencing the SEC61A1 gene in HeLa cells inhibited co- and post-translational transport of signal-peptide-containing precursor proteins into the ER of semi-permeabilized cells, silencing the SEC61A1 gene did not affect transport of various types of tail-anchored protein. Furthermore, we demonstrated, with a similar knockdown approach, a precursor-specific involvement of mammalian Sec63 in the initial phase of co-translational protein transport into the ER. By contrast, silencing the SEC62 gene inhibited only post-translational transport of a signal-peptide-containing precursor protein. © 2012.

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