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Cuiabá, Brazil

da Silva Lemos L.,State University of Norte Fluminense | dos Santos A.S.O.,State University of Norte Fluminense | Rodrigues A.B.F.,State University of Norte Fluminense | Goulart M.L.V.S.,Medica Veterinaria | And 2 more authors.
International Journal of Morphology

A dog with multiple infection by D. renale in the abdominal cavity presented granular peritonitis with giant cells phagocytizing D. renale eggs. Hepatic and phrenic serositis associated to numerous eggs of the parasite immersed in fibrinous process were observed. Lungs presented D. renale eggs in the parenchyma, mononucleated cell infiltrates, edema, hemorrhage, congestion, atelectasia, emphysema, and thromboembolism. D. renale eggs were detected inside the center-lobular veins, auricular cavities, and superficial venous bed of the heart. These findings characterized an atypical dissemination pathway of eggs in erratic cycle. Source

De Castro C.C.,Federal University of Pelotas | Johann J.M.,Medica Veterinaria | Finger P.F.,Federal University of Pelotas | Nunes C.F.,Federal University of Pelotas | And 3 more authors.
Semina:Ciencias Agrarias

Canine coronavirus (CCoV) has been reported causing enteric disease mainly in young pups. In this study, to investigate immunity and exposure to CCoV, was estimated the frequency of serum antibodies to CCoV in 121 dogs in the Pelotas, South of Brazil, by serum neutralization test (SN): 22 had not been vaccinated, 69 had been vaccinated at least once, and 30 had unknown vaccination history. Antibodies were present in 47,8% (33/69) of the vaccinated dogs, in 45,5% (10/22) of the unvaccinated, and in 43,3% (13/30) of the dogs with unknown historical vaccination. There was no significant relationship between these antibodies and sex, age, habitat, and season of collection. The results proved the circulation of the CCoV among this dog population and indicated that the infection affects a significant group of animals. The large proportion of seronegative vaccinated dogs indicates failure of CCoV vaccine in inducing neutralizing antibodies, suggesting that immunizations to CCoV should be reevaluated. The authors indicate the need for further studies in order to evaluate the impact of the infection caused by CCoV, as well as to propose and evaluate preventive measures. Source

Leca Junior N.F.,Mestre em Ciencia Animal | Guedes P.E.B.,State University of Santa Cruz | Santana L.N.,Medica Veterinaria | Carvalho F.S.,State University of Santa Cruz | And 4 more authors.
Acta Tropica

Leishmaniosis is a zoonosis caused by protozoa of the genus Leishmania. American cutaneous leishmaniosis (ACL) is mainly caused by the species L. amazonensis and L. braziliensis, and American visceral leishmaniosis (AVL) is caused by L. infantum chagasi. In addition to their proven roles as reservoirs of AVL, dogs are also suspected by researchers to be reservoirs of ACL due to reports of this infection in domestic environments and of infected dogs in endemic areas. The aim of this study was to detect Leishmania sp. infection in dogs from Vila Operária, Buerarema, Bahia, using parasitological tests, indirect immunofluorescent assay (IFA) and polymerase chain reaction (PCR). Furthermore, this study also aimed to identify risk factors associated with illness in dogs in this locality by conducting an epidemiological survey. For this purpose, 292 dogs were clinically evaluated for the presence of skin lesions, and the dogs that showed these changes were submitted to scarification injury to enable preparation of slides for microscopic study of amastigotes. Subsequently, the dogs underwent blood sampling for serological (IFA) and molecular (PCR) tests. Additionally, the owners of the dogs answered an epidemiological questionnaire to facilitate the identification of risk factors for exposure of dogs to pathogens of ACL. Of the 292 dogs studied, 13 (4.5%) had lesions suggestive of ACL, but with a negative parasitological examination and 147 (50.3%) were seropositive according to the IFA. Of the 273 dogs studied using PCR test, 10 (3.66%) were positive for L. braziliensis, and all samples were negative for L. infantum chagasi. Wastelands in the peridomicile and the presence of light in the household were risk factors associated with ACL. The results show that Vila Operária has asymptomatic dogs with ACL and that the detection sensitivity of the IFA was higher than that of PCR for the infected dogs. © 2015 Elsevier B.V. Source

Brizio A.P.D.R.,Grande Rio University | De Salles B.P.,Medica Veterinaria | Prentice C.,Grande Rio University
Semina:Ciencias Agrarias

Chicken feet are products with great commercial importance for the eastern markets. Although Brazil is a large exporter of these products to those markets, little information is available on the sanitary quality of these products. The objective of this study was to evaluate the microbiological quality of frozen chicken feet for human consumption. This study was developed in a slaughterhouse under Federal Inspection, located in the state of Rio Grande do Sul. A total of 98 samples of frozen chicken feet were analyzed, between January and December 2011, for the detection of Salmonella spp., total count of mesophilic bacteria, total coliforms, fecal coliforms, Escherichia coli, Staphylococcus coagulase positive and Clostridium perfringens. About 99% of the results were within the microbiological standards established by the Chinese (world's largest importer) and Brazilian legislation for raw chicken meat. Thus, we conclude that the samples of frozen chicken feet showed satisfactory microbiological quality and no risk to consumer health. Source

Lobato L.P.,University of Porto | Santos M.C.T.R.D.T.,Medica Veterinaria
Revista de Alimentacao Humana

Over the last years, food safety has been considered the essential factor for health promotion. The supply of meals at the schools, should guarantee a nutritionally balanced meal that suits students needs, following the general food hygiene and safety norms. In this context, two systematic and quantified girds able to objectively analyze the hygiene, physical facilities and environment and sanitary conditions of the school canteens, either individually or grouped, were developed, allowing characterizing the studied establishments. Moreover, it is an enormous effort for the standardization of evaluation criteria, as needed to properly and consistently meet the reality of this sector, allowing acting at the level of Food Safety, as a priority, and the Food Quality, as added value. Source

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