Cuiabá, Brazil
Cuiabá, Brazil

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Brizio A.P.D.R.,Grande Rio University | De Salles B.P.,Medica Veterinaria | Prentice C.,Grande Rio University
Semina:Ciencias Agrarias | Year: 2013

Chicken feet are products with great commercial importance for the eastern markets. Although Brazil is a large exporter of these products to those markets, little information is available on the sanitary quality of these products. The objective of this study was to evaluate the microbiological quality of frozen chicken feet for human consumption. This study was developed in a slaughterhouse under Federal Inspection, located in the state of Rio Grande do Sul. A total of 98 samples of frozen chicken feet were analyzed, between January and December 2011, for the detection of Salmonella spp., total count of mesophilic bacteria, total coliforms, fecal coliforms, Escherichia coli, Staphylococcus coagulase positive and Clostridium perfringens. About 99% of the results were within the microbiological standards established by the Chinese (world's largest importer) and Brazilian legislation for raw chicken meat. Thus, we conclude that the samples of frozen chicken feet showed satisfactory microbiological quality and no risk to consumer health.


PubMed | State University of Santa Cruz, Medica Veterinaria and Mestre em Ciencia Animal
Type: | Journal: Acta tropica | Year: 2015

Leishmaniosis is a zoonosis caused by protozoa of the genus Leishmania. American cutaneous leishmaniosis (ACL) is mainly caused by the species L. amazonensis and L. braziliensis, and American visceral leishmaniosis (AVL) is caused by L. infantum chagasi. In addition to their proven roles as reservoirs of AVL, dogs are also suspected by researchers to be reservoirs of ACL due to reports of this infection in domestic environments and of infected dogs in endemic areas. The aim of this study was to detect Leishmania sp. infection in dogs from Vila Operria, Buerarema, Bahia, using parasitological tests, indirect immunofluorescent assay (IFA) and polymerase chain reaction (PCR). Furthermore, this study also aimed to identify risk factors associated with illness in dogs in this locality by conducting an epidemiological survey. For this purpose, 292 dogs were clinically evaluated for the presence of skin lesions, and the dogs that showed these changes were submitted to scarification injury to enable preparation of slides for microscopic study of amastigotes. Subsequently, the dogs underwent blood sampling for serological (IFA) and molecular (PCR) tests. Additionally, the owners of the dogs answered an epidemiological questionnaire to facilitate the identification of risk factors for exposure of dogs to pathogens of ACL. Of the 292 dogs studied, 13 (4.5%) had lesions suggestive of ACL, but with a negative parasitological examination and 147 (50.3%) were seropositive according to the IFA. Of the 273 dogs studied using PCR test, 10 (3.66%) were positive for L. braziliensis, and all samples were negative for L. infantum chagasi. Wastelands in the peridomicile and the presence of light in the household were risk factors associated with ACL. The results show that Vila Operria has asymptomatic dogs with ACL and that the detection sensitivity of the IFA was higher than that of PCR for the infected dogs.


Marques A.L.A.,Federal University of Campina Grande | De Oliveira Assis A.C.,Medica Veterinaria | Simoes S.V.D.,Federal University of Campina Grande | De Lima Tolentino M.L.D.,Federal University of Campina Grande | De Azevedo S.S.,Federal University of Campina Grande
Semina:Ciencias Agrarias | Year: 2016

Bovine viral diarrhea virus (BVDV) has become an important viral infectious agent in cows and is considered endemic in many regions. This study aimed to determine the epidemiological status of BVDV in bovine herds in the semiarid of the state of Paraíba, in northeastern Brazil, by determining the frequency of anti-BVDV antibodies and risk factors associated with the infection. We tested 359 cows from 20 farms. Three had a history of clinically suspected bovine viral diarrhea (BVD) or mucosal disease (MD), whereas the other 17 locations were randomly selected. Detection of anti-BVDV antibodies was performed by indirect ELISA using a commercial kit (IDEXX BVDV p80 Ab Test). Risk factor analysis was performed in two steps, univariate and multivariate analysis. Seven samples were considered suspicious and were excluded from the analysis of seropositive animals. Of 352 animals, 141 (40.1 %; 95% CI = 34. 9% - 45.2 %) were seropositive. Of the 20 farms analyzed, 19 (95%) had seropositive animals and the frequency of seropositivity per farm varied between 4.5 % and 85.7 %. The risk factors identified were as follows: area ≤ 120 hectares (OR = 3.06; 95% CI = 1.43 - 6.53), high animal density (OR = 3.48; 95% CI = 1.24-9.79), weaning age ≤ 60 days (OR = 10.99; 95% CI = 1.31-91.9), exchange of animals (OR = 4.95; 95% CI = 2.08-11.8), calf mortality > 5% (OR = 2.33; 95% CI = 1.07-5.11), and use of natural breeding and artificial insemination (OR = 3.06; 95% CI = 1.22 - 7.67). It was suggested that to avoid the transmission of BVDV infection in bovine herds in the Sertão of Paraíba, it is important to prevent identified associated risk factors and to perform further studies, identifying persistently infected animals.


Komatsu R.S.,Medica Veterinaria | Rodrigues M.A.M.,Federal University of Uberlandia | Loreno W.B.N.,Medica Veterinaria | Santos K.A.,Tecnica em Laboratorio
Bioscience Journal | Year: 2010

This study aimed to evaluate the presence of Staphylococcus coagulase positiva in craft cheeses produced in the city of Uberlândia-MG and to determine the incidence of samples inside and outside the standards allowed by law. We analyzed 50 samples of fresh Minas cheese, the results indicated that 88% of samples were out the standard established by the Ministry of Health for this microorganism. From these results we can conclude that the cheeses produced in the city Uberlândia not meet legal standards concerning the presence of Staphylococcus coagulase-positive, representing a health hazard.


Correa Scheffer Ferreira K.,Instituto Pasteur | Iamamoto K.,Instituto Pasteur | Miyuki Asano K.,Instituto Pasteur | Mori E.,Instituto Pasteur | And 3 more authors.
Revista Peruana de Medicina Experimental y Salud Publica | Year: 2014

Rabies continues to be a challenge for public health authorities and a constraint to the livestock industry in Latin America. Wild and domestic canines and vampire bats are the main transmitter species and reservoirs of the disease. Currently, variations observed in the epidemiological profile of rabies, where the species of hematophagous bat Desmodus rotundus constitutes the main transmitting species. Over the years, knowledge has accumulated about the ecology, biology and behavior of this species and the natural history of rabies, which should lead to continuous development of methods of population control of d. Rotundus as well as prevention and diagnostic tools for rabies. Ecological relationships of this species with other hematophagous and non-hematophagous bats is unknown, and there is much room for improvement in reporting systems and surveillance, as well as creating greater awareness among the farming community. Understanding the impact of human-induced environmental changes on the rabies virus in bats should be cause for further investigation. This will require a combination of field studies with mathematical models and new diagnostic tools. This review aims to present the most relevant issues on the role of hematophagous bats as reservoirs and transmitters of the rabies virus.


Lobato L.P.,University of Porto | Santos M.C.T.R.D.T.,Medica Veterinaria
Revista de Alimentacao Humana | Year: 2010

Over the last years, food safety has been considered the essential factor for health promotion. The supply of meals at the schools, should guarantee a nutritionally balanced meal that suits students needs, following the general food hygiene and safety norms. In this context, two systematic and quantified girds able to objectively analyze the hygiene, physical facilities and environment and sanitary conditions of the school canteens, either individually or grouped, were developed, allowing characterizing the studied establishments. Moreover, it is an enormous effort for the standardization of evaluation criteria, as needed to properly and consistently meet the reality of this sector, allowing acting at the level of Food Safety, as a priority, and the Food Quality, as added value.


Rossi C.A.,Federal University of Santa Maria | Soares M.,Federal University of Santa Maria | Luchese F.C.,Medica Veterinaria | Santurio J.M.,Federal University of Santa Maria
Ciencia Animal Brasileira | Year: 2015

This study evaluated the liquid emulsion of oregano and rosemary essential oils in the control of neonatal diarrhea in piglets. We monitored 1334 piglets with average weight of 1.5kg regarding the clinical signs of neonatal diarrhea. The design was completely randomized with three treatments (T): (T1) control (saline), (T2) essential oils and (T3) enrofloxacin. T1 showed characteristic ES clinical signs of diarrhea differing (P <0.01) from other treatments. The cure rate of diarrhea was not different (P>0.01) between piglets receiving essential oils or antibiotic, regardless of the order of parturition of the sows. On the other hand, piglets with clinical signs of diarrhea born to sows with order of parturition less than three had a higher cure rate (74.6%) compared with diarrheal piglets born to sows with order of parturition between three and five (68.2%) and more than five (56.4%). The counting of heterotrophic bacteria colony, Escherichia coli and Clostridium perfringens, and histological analyzes of the ileal epithelium showed no differences (P>0.01) among treatments. The emulsion with essential oils of oregano and rosemary (2mL kg-1), for piglets between zero and eight days of age, might replace the enrofloxacin in controlling clinical signs of neonatal diarrhea.


Santos V.V.M.,Medica Veterinaria | De Moura F.M.L.,Medica Veterinaria | De Arreguy Baptista R.I.A.,Federal Rural University of Pernambuco
Acta Veterinaria Brasilica | Year: 2015

The Art and Craft Fair of Boa Viagem in Recife - PE/ Brazil is much visited mainly due to their trade of typical foods. Considering, among other things, the constant risk of food contamination at the site aimed to assess the sanitary conditions of the food trade. For this, we used a checklist based on the checklist contained in Collegiate Board Resolution (RDC) n 275/02 and the determinations of RDC n° 216/04, both the National Health Surveillance Agency (Anvisa),Ministry of Health. The checklist was divided into four blocks: Exhibition of food for sale and / or consumption; Food preparation; Food handlers; and Tent facilities. From the results obtained, the tents were classified into three groups according to the percentage of items served. After analyzing the results, it was found that 25% of the blocks showed high attendance to the items evaluated, and others 75% medium and low attendance. So great are the possibilities of food contamination and hence the occurrence of foodborne diseases. It is necessary, therefore, that changes occur to the adequacy of food services to existing legislation through local infrastructure improvements, training of handlers and joint action by the regulatory agencies in order to provide safe food for the population.


da Silva Lemos L.,State University of Norte Fluminense | dos Santos A.S.O.,State University of Norte Fluminense | Rodrigues A.B.F.,State University of Norte Fluminense | Goulart M.L.V.S.,Medica Veterinaria | And 2 more authors.
International Journal of Morphology | Year: 2010

A dog with multiple infection by D. renale in the abdominal cavity presented granular peritonitis with giant cells phagocytizing D. renale eggs. Hepatic and phrenic serositis associated to numerous eggs of the parasite immersed in fibrinous process were observed. Lungs presented D. renale eggs in the parenchyma, mononucleated cell infiltrates, edema, hemorrhage, congestion, atelectasia, emphysema, and thromboembolism. D. renale eggs were detected inside the center-lobular veins, auricular cavities, and superficial venous bed of the heart. These findings characterized an atypical dissemination pathway of eggs in erratic cycle.


PubMed | Instituto Pasteur and Medica veterinaria
Type: Journal Article | Journal: Revista peruana de medicina experimental y salud publica | Year: 2014

Rabies continues to be a challenge for public health authorities and a constraint to the livestock industry in Latin America. Wild and domestic canines and vampire bats are the main transmitter species and reservoirs of the disease. Currently, variations observed in the epidemiological profile of rabies, where the species of hematophagous bat Desmodus rotundus constitutes the main transmitting species. Over the years, knowledge has accumulated about the ecology, biology and behavior of this species and the natural history of rabies, which should lead to continuous development of methods of population control of d. Rotundus as well as prevention and diagnostic tools for rabies. Ecological relationships of this species with other hematophagous and non-hematophagous bats is unknown, and there is much room for improvement in reporting systems and surveillance, as well as creating greater awareness among the farming community. Understanding the impact of human-induced environmental changes on the rabies virus in bats should be cause for further investigation. This will require a combination of field studies with mathematical models and new diagnostic tools. This review aims to present the most relevant issues on the role of hematophagous bats as reservoirs and transmitters of the rabies virus.

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