Tebbe M.,University of Bayreuth |
Lentz S.,University of Bayreuth |
Guerrini L.,Medcom Advance |
Fery A.,University of Bayreuth |
And 6 more authors.
Discrete gold nanoparticle crystals with tunable size and morphology are fabricated via a fast and inexpensive template-assisted method. The highly precise hierarchical organization of the plasmonic building blocks yields superstructures with outstanding behaviour for surface-enhanced Raman scattering analysis. © The Royal Society of Chemistry 2016. Source
Morla-Folch J.,Medcom Advance |
Morla-Folch J.,Rovira i Virgili University |
Xie H.-N.,Medcom Advance |
Gisbert-Quilis P.,Medcom Advance |
And 9 more authors.
Angewandte Chemie - International Edition
Recognition of chemical modifications in canonical nucleobases of nucleic acids is of key importance since such modified variants act as different genetic encoders, introducing variability in the biological information contained in DNA. Herein, we demonstrate the feasibility of direct SERS in combination with chemometrics and microfluidics for the identification and relative quantification of 4 different cytosine modifications in both single- and double-stranded DNA. The minute amount of DNA required per measurement, in the sub-nanogram regime, removes the necessity of pre-amplification or enrichment steps (which are also potential sources of artificial DNA damages). These findings show great potentials for the development of fast, low-cost and high-throughput screening analytical devices capable of detecting known and unknown modifications in nucleic acids (DNA and RNA) opening new windows of activity in several fields such as biology, medicine and forensic sciences. © 2015 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Source
Mir-Simon B.,Medcom Advance |
Reche-Perez I.,Medcom Advance |
Reche-Perez I.,Rovira i Virgili University |
Guerrini L.,Medcom Advance |
And 5 more authors.
Chemistry of Materials
Encoded particles are one of the most powerful approaches for multiplex high-throughput screening. Surface-enhanced Raman scattering (SERS) based codification can, in principle, avoid many of the intrinsic limitations due to conventional alternatives, as it decreases the reading time and particle size while allowing for almost unlimited codification. Unfortunately, methods for the synthetic preparation of these particles are tedious; often subjected to limited reproducibility (associated with large fluctuations in the size distributions of the polymers employed in the standard protocols); and to date, limited to a small amount of molecules. Herein, we report a universal, one-pot, inexpensive, and scalable synthetic protocol for the fabrication of SERS-encoded nanoparticles. This synthetic strategy is highly reproducible, independent of the chemical nature and size of the Raman code used (31 different codes were tested) and scalable in the liter range without affecting the final properties of the encoded structures. Furthermore, the SERS efficiency of the fabricated encoded nanoparticles is superior to that of the materials produced by conventional methods, while showing a remarkable reproducibility from batch to batch. This encoding strategy can easily be applied to nanoparticles of different materials and shapes. © 2015 American Chemical Society. Source