Viladecans, Spain
Viladecans, Spain

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Morla-Folch J.,Medcom Advance | Morla-Folch J.,Rovira i Virgili University | Xie H.-N.,Medcom Advance | Gisbert-Quilis P.,Medcom Advance | And 9 more authors.
Angewandte Chemie - International Edition | Year: 2015

Recognition of chemical modifications in canonical nucleobases of nucleic acids is of key importance since such modified variants act as different genetic encoders, introducing variability in the biological information contained in DNA. Herein, we demonstrate the feasibility of direct SERS in combination with chemometrics and microfluidics for the identification and relative quantification of 4 different cytosine modifications in both single- and double-stranded DNA. The minute amount of DNA required per measurement, in the sub-nanogram regime, removes the necessity of pre-amplification or enrichment steps (which are also potential sources of artificial DNA damages). These findings show great potentials for the development of fast, low-cost and high-throughput screening analytical devices capable of detecting known and unknown modifications in nucleic acids (DNA and RNA) opening new windows of activity in several fields such as biology, medicine and forensic sciences. © 2015 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.


Morla-Folch J.,Medcom Advance | Morla-Folch J.,Rovira i Virgili University | Alvarez-Puebla R.A.,Medcom Advance | Alvarez-Puebla R.A.,Rovira i Virgili University | And 3 more authors.
Journal of Physical Chemistry Letters | Year: 2016

Design of ultrasensitive DNA sensors based on the unique physical properties of plasmonic nanostructures has become one of the most exciting areas in nanomedicine. However, despite the vast number of proposed applications, the determination of the base composition in nucleic acids, a fundamental parameter in genomic analyses and taxonomic classification, is still restricted to time-consuming and poorly sensitive conventional methods. Herein, we demonstrate the possibility of determining the base composition in single- and double-stranded DNA by using a simple, low-cost, high-throughput, and label-free surface-enhanced Raman scattering (SERS) method in combination with cationic nanoparticles. © 2016 American Chemical Society.


Mir-Simon B.,Medcom Advance | Reche-Perez I.,Medcom Advance | Reche-Perez I.,Rovira i Virgili University | Guerrini L.,Medcom Advance | And 5 more authors.
Chemistry of Materials | Year: 2015

Encoded particles are one of the most powerful approaches for multiplex high-throughput screening. Surface-enhanced Raman scattering (SERS) based codification can, in principle, avoid many of the intrinsic limitations due to conventional alternatives, as it decreases the reading time and particle size while allowing for almost unlimited codification. Unfortunately, methods for the synthetic preparation of these particles are tedious; often subjected to limited reproducibility (associated with large fluctuations in the size distributions of the polymers employed in the standard protocols); and to date, limited to a small amount of molecules. Herein, we report a universal, one-pot, inexpensive, and scalable synthetic protocol for the fabrication of SERS-encoded nanoparticles. This synthetic strategy is highly reproducible, independent of the chemical nature and size of the Raman code used (31 different codes were tested) and scalable in the liter range without affecting the final properties of the encoded structures. Furthermore, the SERS efficiency of the fabricated encoded nanoparticles is superior to that of the materials produced by conventional methods, while showing a remarkable reproducibility from batch to batch. This encoding strategy can easily be applied to nanoparticles of different materials and shapes. © 2015 American Chemical Society.


Tebbe M.,University of Bayreuth | Lentz S.,University of Bayreuth | Guerrini L.,Medcom Advance | Fery A.,University of Bayreuth | And 6 more authors.
Nanoscale | Year: 2016

Discrete gold nanoparticle crystals with tunable size and morphology are fabricated via a fast and inexpensive template-assisted method. The highly precise hierarchical organization of the plasmonic building blocks yields superstructures with outstanding behaviour for surface-enhanced Raman scattering analysis. © The Royal Society of Chemistry 2016.


Torres-Nunez A.,Medcom Advance | Torres-Nunez A.,Centro Tecnologico Of La Quimica De | Faulds K.,University of Strathclyde | Graham D.,University of Strathclyde | And 5 more authors.
Analyst | Year: 2016

Ultrasensitive direct SERS analysis offers a powerful analytical tool for the structural characterization and classification of nucleic acids. However, acquisition of reliable spectral fingerprints of such complex biomolecules poses important challenges. In recent years, many efforts have been devoted to overcome these limitations, mainly implementing silver colloids as plasmonic substrates. However, a reliable cross-comparison of results reported in the recent literature is extremely hard to achieve, mostly due to the broad set of different surface properties of the plasmonic nanoparticles. Herein, we perform a thorough investigation of the role played by the metal/liquid interface composition of silver colloids in the direct label-free SERS analysis of DNA. Target molecules of increasing complexity, from short homopolymeric strands to long genomic duplexes, were used as probes. We demonstrate how apparently subtle changes in the colloidal surface chemistry can dramatically modify the affinity and the final SERS spectral profile of DNA. This has significant implications for the future design of new analytical strategies for the detection of DNA using SERS without labels. © 2016 The Royal Society of Chemistry.


PubMed | Medcom Advance
Type: Journal Article | Journal: Angewandte Chemie (International ed. in English) | Year: 2015

Recognition of chemical modifications in canonical nucleobases of nucleic acids is of key importance since such modified variants act as different genetic encoders, introducing variability in the biological information contained in DNA. Herein, we demonstrate the feasibility of direct SERS in combination with chemometrics and microfluidics for the identification and relative quantification of 4 different cytosine modifications in both single- and double-stranded DNA. The minute amount of DNA required per measurement, in the sub-nanogram regime, removes the necessity of pre-amplification or enrichment steps (which are also potential sources of artificial DNA damages). These findings show great potentials for the development of fast, low-cost and high-throughput screening analytical devices capable of detecting known and unknown modifications in nucleic acids (DNA and RNA) opening new windows of activity in several fields such as biology, medicine and forensic sciences.


PubMed | Medcom Advance
Type: Journal Article | Journal: ACS nano | Year: 2016

As more biological activities of ribonucleic acids continue to emerge, the development of efficient analytical tools for RNA identification and characterization is necessary to acquire an in-depth understanding of their functions and chemical properties. Herein, we demonstrate the capacity of label-free direct surface-enhanced Raman scattering (SERS) analysis to access highly specific structural information on RNAs at the ultrasensitive level. This includes the recognition of distinctive vibrational features of RNAs organized into a variety of conformations (micro-, fully complementary duplex-, small interfering- and short hairpin-RNAs) or characterized by subtle chemical differences (single-base variances, nucleobase modifications and backbone composition). This method represents a key advance in the ribonucleic acid analysis and will have a direct impact in a wide range of different fields, including medical diagnosis, drug design, and biotechnology, by enabling the rapid, high-throughput, simple, and low-cost identification and classification of structurally similar RNAs.

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