Medallion Laboratories General Mills Inc.

Minneapolis, MN, United States

Medallion Laboratories General Mills Inc.

Minneapolis, MN, United States

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McCleary B.V.,Megazyme International | DeVries J.W.,Medallion Laboratories General Mills Inc. | Rader J.I.,U.S. Food and Drug Administration | Cohen G.,Kraft Foods Inc. | And 4 more authors.
Journal of AOAC International | Year: 2010

A method for the determination of total dietary fiber (TDF), as defined by the CODEX Alimentarius, was validated in foods. Based upon the principles of AOAC Official MethodsSM 985.29, 991.43, 2001.03, and 2002.02, the method quantitates high- and low-molecular-weight dietary fiber (HMWDF and LMWDF, respectively). In 2007, McCleary described a method of extended enzymatic digestion at 37°C to simulate human intestinal digestion followed by gravimetric isolation and quantitation of HMWDF and the use of LC to quantitate low-molecular-weight soluble dietary fiber (LMWSDF). The method thus quantitates the complete range of dietary fiber components from resistant starch (by utilizing the digestion conditions of AOAC Method 2002.02) to digestion resistant oligosaccharides (by incorporating the deionization and LC procedures of AOAC Method 2001.03). The method was evaluated through an AOAC collaborative study. Eighteen laboratories participated with 16 laboratories returning valid assay data.


Dorweiler K.J.,Medallion Laboratories General Mills Inc. | Gurav J.N.,Medallion Laboratories General Mills Inc. | Walbridge J.S.,Sigma-Aldrich | Ghatge V.S.,Medallion Laboratories General Mills Inc. | Savant R.H.,Medallion Laboratories General Mills Inc.
Journal of Agricultural and Food Chemistry | Year: 2016

A study was conducted to evaluate the stability of 528 pesticides, metabolites, and contaminants prepared in large multicomponent mixes to enhance laboratory efficiency by allowing maximum use of the useful shelf life of the mixtures. Accelerated aging at 50 °C simulated 6 month, 1 year, and 2 year storage periods at -20 °C. Initial mixture composition was based on the instrument of analysis. After preliminary stability data had been obtained, mixtures were reformulated and re-evaluated. In all, 344 compounds showed satisfactory stability across all treatment groups, 100 compounds showed statistically significant changes between the control and the 6 month simulated storage period (27 with losses >20%), and the remainder showed borderline stability or were tested in one protocol. Stability behavior for organophosphates agreed with the proposed reaction mechanism responsible for acetylcholinesterase inhibition. A small number of compounds increased in response over time, suggesting the occurrence of degradation of precursor pesticides into these respective compounds. © 2016 American Chemical Society.


PubMed | Sigma-Aldrich and Medallion Laboratories General Mills Inc.
Type: Journal Article | Journal: Journal of agricultural and food chemistry | Year: 2016

A study was conducted to evaluate the stability of 528 pesticides, metabolites, and contaminants prepared in large multicomponent mixes to enhance laboratory efficiency by allowing maximum use of the useful shelf life of the mixtures. Accelerated aging at 50 C simulated 6 month, 1 year, and 2 year storage periods at -20 C. Initial mixture composition was based on the instrument of analysis. After preliminary stability data had been obtained, mixtures were reformulated and re-evaluated. In all, 344 compounds showed satisfactory stability across all treatment groups, 100 compounds showed statistically significant changes between the control and the 6 month simulated storage period (27 with losses >20%), and the remainder showed borderline stability or were tested in one protocol. Stability behavior for organophosphates agreed with the proposed reaction mechanism responsible for acetylcholinesterase inhibition. A small number of compounds increased in response over time, suggesting the occurrence of degradation of precursor pesticides into these respective compounds.


Zielinski G.,Covance Laboratories Inc. | De Vries J.W.,Medallion Laboratories General Mills Inc. | Craig S.A.,DuPont Company | Bridges A.R.,AACC International
Cereal Foods World | Year: 2013

Currently there are multiple Type I methods for dietary fiber analysis, which conflicts with the principles of the Codex Procedural Manual (2). While not ideal, CCMAS is using the most recent scientific evidence to simplify and aid those involved in dietary fiber testing. At times, scientific understanding may outpace the rate at which information can be collected, reviewed, and disseminated, but the Codex Alimentarius is progressing using input from world experts in dietary fiber method research. Harmonizing an internationally accepted definition for dietary fiber was a large undertaking that spanned 16 years. Some national authorities have openly accepted this fairly recent definition, whereas others have not commented on the topic for a number of years. To ensure the most appropriate test method is used, analysts, laboratory managers, and others analyzing foods for dietary fiber need to understand how and where the results from the testing will be used and any legal implications that may arise. While CAC is working toward a single analytical method commensurate with its concise definition, there may not be a procedure that accounts for all possible situations. In the interim, CAC and its communities can aid analysts worldwide by providing guidance and information when possible. This report is intended to capture the concerns and issues raised by the electronic working group and during the meetings of CCMAS 33 and can be used to provide guidance and additional information to that supplied in a Codex standard. © 2013 AACC International, Inc.


Wehling P.,General Mills Inc. | DeVries J.W.,Medallion Laboratories General Mills Inc.
Journal of AOAC International | Year: 2012

The quest for optimum methodology for any analyte often includes a study of the method in multiple independent laboratories. Evaluation of the results of these studies is typically subjective; however, various attempts have been made to increase the objectivity of the evaluation. Among the objective propositions is the use of the Horwitz Ratio (HorRat) as applied to collaborative study statistics. A historical review of fiber method validation studies performed since 1940 shows that the Horwitz curve does not effectively predict the results of dietary fiber collaborative studies, either retrospectively or prospectively. Consequently, use of the HorRat as a criterion for accepting or rejecting dietary fiber methods is contraindicated. An alternative, objective statistical approach is proposed that may also apply to collaborative studies of other empirical analytical methods in general.


Post B.E.,Medallion Laboratories General Mills Inc. | Marshak M.R.,Medallion Laboratories General Mills Inc. | DeVries J.W.,Medallion Laboratories General Mills Inc.
Journal of AOAC International | Year: 2010

Dietary fiber and its quantitation in foods have been of significant interest in the nutrition community for over 50 years. A number of AOAC Official Methods of AnalysisSM have been adopted for the analysis of dietary fiber and some of its fractions and components commensurate with the evolving discoveries of dietary fiber nutrition research. Quantitation of low-molecular-weight soluble dietary fiber (LMWSDF) has been difficult due to high solubility in a precipitating solvent mixture of four parts alcohol and one part water. AOAC Method 2001.03 effectively quantitates LWMSDF subsequent to gravimetric removal of high-molecular-weight dietary fiber using LC. However, deionization and concentration of the enzymatic digestate, necessary to assure accurate LC quantitation, requires substantial time and manual labor. A modification to the method and resulting method performance is presented that describes a means of simultaneously deionizing the digestate and quantitating the LMWSDF in a single LC injection, eliminating a number of timeconsuming manual preparation steps.


DeVries J.W.,Medallion Laboratories General Mills Inc.
Journal of AOAC International | Year: 2012

During the "Standards Development and International Harmonization: AOAC INTERNATIONAL Mid-Year Meeting," held on June 29, 2011, an Expert Review Panel (ERP) reviewed the method for the "Determination of Vitamins A (Retinol) and E (alpha-Tocopherol) in Foods by Liquid Chromatography: Collaborative Study," published by Jonathan W. DeVries and Karlene R. Silvera in J. AOAC Int. in 2002. After evaluation of the original validation data, an ERP agreed in June 2011 that the method meets standard method performance requirements (SMPRs) for vitamin A, as articulated by the Stakeholder Panel on Infant Formula and Adult Nutritionals. The ERP granted the method First Action status, applicable to determining vitamin A in ready-to-eat infant and adult nutritional formula. In an effort to achieve Final Action status, it was recommended that additional information be generated for different types of infant and adult nutritional formula matrixes at varied concentration levels as indicated in the vitamin A (retinol) SMPR. Existing AOAC LC methods are suited for specific vitamin A analytical applications. The original method differs from existing methods in that it can be used to assay samples in all nine sectors of the food matrix. One sector of the food matrix was powdered infant formula and gave support for the First Action approval for vitamin A in infant and adult nutritional formula. In this method, standards and test samples are saponified in basic ethanol-water solution, neutralized, and diluted, converting fats to fatty acids and retinol esters to retinol. Retinol is quantitated by an LC method, using UV detection at 313 or 328 nm for retinol. Vitamin concentration is calculated by comparison of the peak heights or peak areas of retinol in test samples with those of standards.


Szpylka J.,Medallion Laboratories General Mills Inc. | Szpylka J.,Silliker Laboratories | DeVries J.,Medallion Laboratories General Mills Inc. | Cheney A.,Medallion Laboratories General Mills Inc. | House S.,Medallion Laboratories General Mills Inc.
Journal of AOAC International | Year: 2012

The method for "Determination of Total Folates in Infant Formula and Adult Nutritionals by Trienzyme Extraction and UPLC-MS/MS Quantitation" was submitted to the Folate Working Group for consideration for adoption as Official First Action by AOAC INTERNATIONAL. This method uses ultra performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) to determine the total folates in infant formulas and adult nutritionals after trienzyme digestion. Deconjugation of the various folate polyglutamates to folate monoglutamates is achieved by using rat plasma conjugase after the sample digestion with protease and a-amylase during the trienzyme digestion process. This method shows linearity of folate concentrations in the range of 10-19 100 μg/100 g. Extension of the range to cover folate concentrations of 5-2 000 000 μg/100 g can be achieved with appropriate adjustment of the sample weight and SPE cleanup loading volume. The recoveries ranged from 94.10 to 101.34%.


PubMed | Medallion Laboratories General Mills Inc.
Type: Journal Article | Journal: Journal of AOAC International | Year: 2013

The method for Determination of Total Folates in Infant Formula and Adult Nutritionals by Trienzyme Extraction and UPLC-MS/MS Quantitation was submitted to the Folate Working Group for consideration for adoption as Official First Action by AOAC INTERNATIONAL. This method uses ultra performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) to determine the total folates in infant formulas and adult nutritionals after trienzyme digestion. Deconjugation of the various folate polyglutamates to folate monoglutamates is achieved by using rat plasma conjugase after the sample digestion with protease and a-amylase during the trienzyme digestion process. This method shows linearity of folate concentrations in the range of 10-19 100 microg/100 g. Extension of the range to cover folate concentrations of 5-2 000 000 microg/100 g can be achieved with appropriate adjustment of the sample weight and SPE cleanup loading volume. The recoveries ranged from 94.10 to 101.34%.


PubMed | Medallion Laboratories General Mills Inc.
Type: Journal Article | Journal: Journal of AOAC International | Year: 2013

A colorimetric method for the determination of total antioxidant activity in a variety of foods and beverages was validated in both a single-laboratory validation and a collaborative laboratory validation study. The procedure involved extraction of the antioxidants directly into a methanol-water solution containing a known amount of 2,2-diphenyl-1-picrylhydrazyl (DPPH), thus promoting the rapid reaction of extracted materials with DPPH. The reaction was monitored by spectrophotometric measurement of the absorbance loss at 517 nm. Antioxidant activity was quantified relative to a dilution series of vitamin E analog standards (Trolox), which were analyzed in parallel simultaneously with the food and beverage samples. The antioxidant activities of the samples ranged from 131 to 131 000 micromole Trolox equivalents/100 g. Statistical analysis of the results showed that nine of the 11 matrixes gave acceptable HorRat values, indicating that the method performed well in these cases. The acceptable matrixes include pomegranate juice, blueberry juice, carrot juice, green tea, wine, rosemary spice, ready-to-eat cereal, and yogurt. Two samples failed the HorRat test: the first was an almond milk that had an antioxidant level below the practical LOQ for the method; the second was a sample of canola oil with added omega-3 fatty acid that was immiscible in the reaction medium.

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