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Vasiliev Y.M.,Mechnikov Research Institute of Vaccines and Sera
Expert Review of Vaccines | Year: 2014

A number of preclinical and clinical studies with chitosan-adjuvanted antigen- and DNA-based vaccines have been carried out. Various chitosans and their modifications, in different forms (solutions, powders, gels and particles), have been evaluated with various antigens administered via different routes. Chitosan is a generic name for a wide array of glucosamine-based substances derived from biological sources, and standardization is necessary. However, in most of the studies published to date, molecular weight, viscosity, deacetylation degree and/or purity level (especially endotoxins) are not provided for the initial chitosan substance and/or final formulation and the preparation procedure is not detailed. Evaluation of adjuvant properties is challenging, given that the only available data are insufficient to demonstrate immunogenicity for chitosans with characteristics within certain intervals to elucidate mechanisms of action or to exclude impurities as the active substance. These and other issues of chitosan-based vaccine adjuvants are summarized and a step-by-step evaluation approach for chitosan-based vaccine adjuvants is outlined. © 2015 Informa UK Ltd. Source

Koloskova O.O.,Moscow State University | Nikonova A.A.,NRC Institute of Immunology | Budanova U.A.,Moscow State University | Shilovskiy I.P.,NRC Institute of Immunology | And 7 more authors.
European Journal of Pharmaceutics and Biopharmaceutics | Year: 2016

Nucleic acid-based therapeutics have recently emerged as a new class of next generation agents for treatment and prevention of viral infection, cancer, and genetic disorders, but their wide use is limited by their relatively weak delivery into target cells. Usage of synthetic cationic amphiphiles with peptide hydrophilic domain as agents for non-viral gene delivery is an attractive approach. We developed the schemes for the synthesis of aliphatic peptides with different length of the hydrocarbon chains in hydrophobic domains and different amino acids in polar head. For the obtained derivatives we determined transfection efficiency, critical vesicle concentration, particle size, ζ-potential and aggregates stability. We have found that the transfection efficiency is increased if the ornithine is a part of polar head in an amphiphile. The most promising amphiphile for liposomal formation OrnOrnGlu(C16H33)2 was examined more carefully. It has been shown that the lipopeptide possesses low toxicity (in vitro and in vivo) and high transfection efficiency with pDNA and siRNA in different cell lines. In addition, the production of liposomes based on this lipopeptide is simple, quick and cheap. Thus OrnOrnGlu(C16H33)2 is a promising vehicle for gene delivery and gene silencing. © 2016 Elsevier B.V. All rights reserved. Source

Samoylikov P.,Mechnikov Research Institute of Vaccines and Sera | Gervazieva V.,Mechnikov Research Institute of Vaccines and Sera | Kozhevnikov S.,Research Institute of Pediatrics and Pediatric Surgery
World Allergy Organization Journal | Year: 2013

Background: Patients with atopic dermatitis (AD) can develop autoantibodies against intracellular proteins. AD patients often suffer from herpes viruses (HV) infection which complicates the inflammatory process in the skin. The aim of the study was to reveal IgE and IgG antibodies (abs) specific to some skin antigens and to compare their levels with the severity of AD with HV infection in children. Methods: IgE and IgG abs specific to tissue antigens, total IgE, IgE-abs to environmental common allergens as well as IgG abs specific to HV were detected in serum samples by ELISA in 157 AD children. Results: IgE and IgG antibody production to keratin and elastin was observed in children with AD and elevated proportionally to the severity of AD. IgG - abs to herpes simplex virus was increased in children and associated with the severity of clinical course of AD. Conclusion: Our data shown that clinical course of severe AD is accompanied with autoimmune response to epidermal antigens (keratin and elastin). Elevated levels of the autoantibodies, especially against the background of HV infection may be useful serological parameter for monitoring of the disease activity. © 2013 Assarehzadegan et al.; licensee BioMed Central Ltd. Source

Dmitriev A.D.,Mechnikov Research Institute of Vaccines and Sera | Tarakanova J.N.,Mechnikov Research Institute of Vaccines and Sera | Yakovleva D.A.,Mechnikov Research Institute of Vaccines and Sera | Dmitriev D.A.,Mechnikov Research Institute of Vaccines and Sera | And 8 more authors.
Journal of Immunoassay and Immunochemistry | Year: 2013

This article reexamines some opinions concerning pH requirements for optimal immobilization of monoclonal antibodies (mAbs) by passive adsorption in antigen capture ELISA. It was discovered that substitution of "classical" sodium phosphate (pH 7.5) and carbonate (pH 9.5) coating solutions by acid (pH 2.8) buffers maximized antigen capture 4 out of 10 different tested anti-HBsAg mAbs, resulting in a 1.5-2.5 increase of binding curve coefficients. By measuring both mAbs amounts and functionality, the enhancement effect was attributed to the better preservation of solid phase antibodies activity. Copyright © Taylor & Francis Group, LLC. Source

Krylov V.,Mechnikov Research Institute of Vaccines and Sera | Shaburova O.,Mechnikov Research Institute of Vaccines and Sera | Krylov S.,Mechnikov Research Institute of Vaccines and Sera | Pleteneva E.,Mechnikov Research Institute of Vaccines and Sera
Viruses | Year: 2012

Pseudomonas aeruginosa is a frequent participant in wound infections. Emergence of multiple antibiotic resistant strains has created significant problems in the treatment of infected wounds. Phage therapy (PT) has been proposed as a possible alternative approach. Infected wounds are the perfect place for PT applications, since the basic condition for PT is ensured; namely, the direct contact of bacteria and their viruses. Plenty of virulent ("lytic") and temperate ("lysogenic") bacteriophages are known in P. aeruginosa. However, the number of virulent phage species acceptable for PT and their mutability are limited. Besides, there are different deviations in the behavior of virulent (and temperate) phages from their expected canonical models of development. We consider some examples of non-canonical phage-bacterium interactions and the possibility of their use in PT. In addition, some optimal approaches to the development of phage therapy will be discussed from the point of view of a biologist, considering the danger of phage-assisted horizontal gene transfer (HGT), and from the point of view of a surgeon who has accepted the Hippocrates Oath to cure patients by all possible means. It is also time now to discuss the possible approaches in international cooperation for the development of PT. We think it would be advantageous to make phage therapy a kind of personalized medicine. © 2013 by the authors, licensee MDPI, Basel, Switzerland. Source

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