Gama Mechatronics Company

Nilüfer, Turkey

Gama Mechatronics Company

Nilüfer, Turkey
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Alpsoy L.,Gama Mechatronics Company | Baykal A.,University of Dammam | Kurtan U.,Istanbul University | Ulker Z.,Gama Mechatronics Company
Journal of Superconductivity and Novel Magnetism | Year: 2017

In this study, a stable carboxylated luteolin (CL)-functionalized superparamagnetic iron oxide nanoparticle (SPION) as a potential drug carrier for in vitro analysis of L929 (mouse fibroblast), U87 (glioblastoma (brain cancer)), MCF-7 (breast cancer), HeLa (cervix cancer), and A549 (human lung cancer) cell lines has been synthesized. Thermal decomposition and Stöber methods were used to prepare 3-aminopropyl triethoxysilane-capped SPIONs respectively. Carboxylated polyethylene glycol (PEG-COOH), folic acid (FA), and CL were conjugated on the surface via a carboxylic/amine group using the nanoprecipitation method respectively. Internalization of CL-functionalized SPION and the release of luteolin from it has been studied using Prussian blue staining and spectrophotometry respectively. The cytotoxicity of CL-functionalized SPION on cell lines was tested by MTT assay. Internalization of product by HeLa, MCF-7, and U87 was higher than A549 and L929 cells. It was observed that luteolin release increased in an acidic environment (pH 5.4). A newly synthesized SPION-FA-PEG in all concentrations (except 500 μg/mL) did not show notable toxicity against L929, U87, MCF-7, HeLa, and A549. However, the product in all used concentrations decreased cell viability at the 24th and 48th hours. This study confirmed that the product has a potential to be used as an anti-cancer CL-functionalized SPION for targeted drug delivery. © 2017 Springer Science+Business Media New York


Alpsoy L.,Gama Mechatronics Company | Baykal A.,University of Dammam | Kurtan U.,Istanbul University | Akal Z.U.,Gama Mechatronics Company
Journal of Superconductivity and Novel Magnetism | Year: 2017

In this research, we synthesized a novel caffeic acid-functionalized iron oxide nanoparticles (CFA-functionalized SPION) L929 (mouse fibroblast cell), U87 (glioblastoma brain cancer cell), MCF-7 (breast cancer cell), HeLa (cervix cancer cell), and A549 (human lung cancer cell) cell lines. Thermal decomposition and Stöber methods were used to prepare APTES-capped SPION, respectively. The carboxylated polyethylene glycol (PEG-COOH), folic acid (FA), and caffeic acid (CFA) were attached to the surface of SPION via carboxylic/amine groups. Structural analysis (Rietveld analysis) confirmed the phase purity of the product. The conjugation of organics to the surface of SPION was followed with FT-IR spectroscopy and thermal gravimetric analysis (TGA). SEM analysis presented the spherical morphology of product with 13 ± 3 nm particle size. And also, superparamagnetic property of product was deduced from VSM analysis. Uptake of CFA-functionalized SPION from the cell and release of CFA from CFA-functionalized SPION has been studied by using Prussian blue staining and spectrophotometer, respectively. Also, cell viability and cytotoxicity was tested by MTT and LDH assays. The uptake of CFA-functionalized SPION by HeLa, MCF-7, and U87 was higher than A549 and L929 cells. Also, caffeic acid release from CFA-functionalized SPION increased at an acidic environment (pH 4.4). A newly synthesized CFA-functionalized SPION in all used concentrations decreased cell viability and increased cytotoxicity at 24th and 48th hours. The results showed that the CFA-functionalized SPION is a potential anticancer agent for cancer therapy. © 2017 Springer Science+Business Media New York


Alpsoy L.,Gama Mechatronics Company | Baykal A.,University of Dammam | Akal Z.U.,Gama Mechatronics Company
Journal of Superconductivity and Novel Magnetism | Year: 2017

The aim of this study is to determine the cytotoxic and apoptotic effects of newly synthesized folic acid (FA) and luteolin (carboxylated luteolin (CL)) conjugated superparamagnetic nanoparticle (SPION@FA-PEG@CL) on folate receptor positive (FAR (+)) and negative (FAR (−)) cell lines in vitro. Cytotoxicity analysis by real-time cell analyzer system (RTCA) and apoptosis by TUNEL and Annexin assay, Caspase 3/7 activities, and Caspase 3/7 expression level were determined. According to RTCA results, SPION@FA-PEG@CL nanodrug showed higher cytotoxicity against U87, MCF-7, HeLa cell lines in comparison with L929 and A549 cell lines. TUNEL and Annexin assays indicated that SPION@FA-PEG@CL increased the apoptotic and necrotic cell ratio in FAR (+) cell. In L929 and A549 cells, are FAR (−) cell lines apoptotic and necrotic cell ratio was lower than FAR (+) cell lines (U87, MCF-7 and HeLa). Caspase 3 and 7 analyses also showed that SPION@FA-PEG@CL nanodrug have apoptotic and necrotic impact on cell lines, they were higher in FAR (+) cells than FAR (−) cells. According to results, synthesized SPION@FA-PEG@CL can be a novel nanodrug delivery system for in cancer chemotherapy. © 2017 Springer Science+Business Media, LLC

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