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Suginami-ku, Japan

Zhan J.,Kyushu University | Furui K.,Konica Minolta | Nakajima H.,Tokyo Metroplitan University | Kaneki N.,Muroran Institute of Technology | And 4 more authors.
Analytical Sciences | Year: 2016

A portable-type surface plasmon resonance (SPR) sensor, composed from a new optical system for multi-sensing, has been developed to apply to environment analysis, clinical diagnosis etc., where many samples are desired to be analyzed at high throughput. The optical system of the sensor consists of a light-emitting diode, a pair of cylindrical lenses, a pair of collimator lenses, a correction lens, a prism, a polarizer and a linear CCD sensor with 2048 pixels. Reflected light from a sensor chip of the width of 6 mm at a certain incident angle was detected by ca. 618 pixels of the linear CCD sensor as an SPR sensor signal. An SPR sensor signal at a specified incident angle is controllable for optimization by adjusting the position of the CCD sensor. A sensor chip having a 30-stripe linear pattern (100 μm width/stripe) was prepared. The spatial resolution as well as the performance of the sensor were evaluated by using sucrose solutions. As a result, the acquisition of SPR sensor signals from 30 sensing points was successfully achieved with a spatial resolution of 100 μm (distance between 2 sensing points). A lower detection limit of ca. 3.2-5.5 × 10-5 RIU with a standard deviation of ±4.5% was obtained by averaging the signals from 6-7 pixels of the CCD sensor per one sensing stripe. 2016. © The Japan Society for Analytical Chemistry. Source


Korenaga A.,Tokyo Metroplitan University | Nakajima H.,Tokyo Metroplitan University | Usui T.,Tokyo Metroplitan University | Morioka K.,Tokyo Metroplitan University | And 4 more authors.
Bunseki Kagaku | Year: 2014

A small amount of nanobeads suspension was dropped onto a partially polymerized PDMS/ Glass plate. The nanobeads in the drop were self-assembled by a capillary force, accompanied by solvent evaporation. Subsequently, a three-dimensional (3D) nanobeads array structure was formed on the plate. The plate with a 3D nanobeads array structure was placed inside an ultrasound bath, and then the nanobeads outside the PDMS layer were removed, while the nanobeads lying on the PDMS layer remained. In this way, a two-dimensional (2D) nanobeads array structure was fabricated. The sensor chip for a transmission-type surface plasmon resonance (SPR) sensor was prepared by forming a thin layer of gold on the 2D nanobeads array structure, and then a novel transmission-type SPR sensor using the sensor chip was developed. The calibration curve for sucrose solutions obtained on the developed SPR sensor was linear for concentrations under 2000 mM with a correlation coefficient of 0.967. In contrast to the sensor chip with a nanohole array structure generally-used for a transmissiontype SPR sensor, the developed sensor chip with the 2D nanobeads array structure has an advantage that the sensor chip can be easily prepared at low cost without the use of an expensive electron beam lithography system. © The Japan Society for Analytical Chemistry 2014. Source


Morioka K.,Tokyo Metroplitan University | Hemmi A.,Mebius Advanced Technology Ltd | Zeng H.,Tokyo Metroplitan University | Uchiyama K.,Tokyo Metroplitan University | Nakajima H.,Tokyo Metroplitan University
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 | Year: 2013

An enzyme-linked immunosorbent assay on a compact disk (CD)-type microfluidic device was developed for the simple and rapid determination of IgA in human saliva, a marker of human stress. The sample, reagent and washing solutions (IgA, IIRP-labeled anti-IgA and Amplex®; Red containing H 2O2) in the reservoirs on the CD-type microfluidic device were sequentially introduced into the detection chamber immobilized with anti-IgA and BSA by the centrifugal force generated by the rotation of the CD-type microfluidic device. To determine IgA, the enzymatic product, resorufin, was detected using a novel fluorescence detection system in conjunction with an OLED and a CCD. Copyright © (2013) by the Chemical and Biological Microsystems Society All rights reserved. All rights reserved. Source


Hemmi A.,Mebius Advanced Technology Ltd | Hemmi A.,Tokyo Metroplitan University | Usui T.,Tokyo Metroplitan University | Moto A.,Tokyo Metroplitan University | And 8 more authors.
Journal of Separation Science | Year: 2011

A surface plasmon resonance (SPR) sensor on a compact disk (CD)-type microfluidic device was developed to miniaturize the elements of a complete analytical system, pump and valves. The CD-type microfluidic device was fabricated by attaching a polydimethylsiloxane disk plate that contained microchannels and reservoirs to a flat polycarbonate disk plate that contained grating films with a thin layer of Au. The optical system of the SPR sensor and the theory for its operation are based on the principle of a grating coupled-type SPR. The sample and reagent solutions in the reservoirs on the CD-type microfluidic device were sequentially introduced into the detection chamber by centrifugal force generated by the rotation of the microfluidic device. The variation of resonance wavelength was dependent on the refractive index of the sample solution. This CD-type SPR sensor was successfully used in an immunoassay of immunoglobulin A (IgA). The anti-IgA, blocking reagent, sample and washing solution in the reservoirs were sequentially introduced into the detection chamber by changing the frequency of rotation of the microfluidic device. IgA in the sample solution was adsorbed to the anti-IgA immobilized on the Au thin layer in the detection chamber and was then detected by the SPR sensor. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source


Morioka K.,Tokyo Metroplitan University | Nakajima H.,Tokyo Metroplitan University | Hemmi A.,Mebius Advanced Technology Ltd | Iida S.,Tokyo Metroplitan University | And 4 more authors.
Bunseki Kagaku | Year: 2016

We have developed a two-dimensional surface plasmon resonance (SPR) sensor using multiplied beam-splitting optics. In this study, we evaluated immunoassays by the SPR sensor. As a result, we could measure the change of the reflected-light intensity in real time, which is based on the immobilization of an antibody onto the surface of the sensor chip, the adsorption of a blocking reagent onto the surface, and specific bonding of the antigen to the antibody immobilized on the sensor chip. The calibration curve for IgA was linear under a concentration of 6.0 μg mL−1, and a quantitative immunoassay was successfully accomplished by the SPR sensor. We also fabricated a sensor chip in which anti-IgA, anti-IgG and anti-IgM were immobilized on the detection point in the flow channel on the sensor chip, respectively. We then evaluated measurements of IgA, IgG and IgM using the sensor chip. When a sample solution containing IgA was introduced into the flow channel, the reflected light intensity at the detection point at which anti-IgA was immobilized dramatically increased, whereas the reflected light intensity at the detection point at which anti-IgG or anti-IgM was immobilized did not change. Similarly, in the case of IgG and IgM, the reflected light intensity only increased at the detection point at which corresponding antibody was immobilized. These results indicate that a simultaneous immunoassay by the SPR sensor should be possible. © 2016 The Japan Society for Analytical Chemistry. Source

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