Mebiol Inc.

Kawasaki, Japan

Mebiol Inc.

Kawasaki, Japan
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Suzuki Y.,Tokyo University of Agriculture and Technology | Suzuki Y.,Waseda University | Ohno S.,Waseda University | Ohno S.,Tokyo Women's Medical University | And 8 more authors.
Anticancer Research | Year: 2012

Background: Although the systemic inflammatory condition can be confirmed in cancer patients, the pathophysiological importance of reactive oxygen species (ROS) produced by neutrophils has not yet been defined. Patients and Methods: Twenty-one patients with inoperable, chemoresistant and radioresistant cancer were enrolled in this study. At least 4 weeks prior to sampling, the patients were free from antitumor treatments. Control samples were also obtained from a healthy donor (39-year-old male). Peripheral blood samples were set 150 μl each on the 2 ml tube with 50 μl Mebiol Gel, and the production of ROS from neutrophils was detected by luminol-dependent chemiluminescence (LmCL) in a kinetic mode at 30-minute intervals for 2.5 hours with a luminometer at 37°C. Results: Each point, peak value and sum of values of LmCL in the patient group was statistically higher than those in the healthy donor. There were no differences in LmCL according to performance status (PS), type of cancer, age, or gender in cancer patients. Conclusion: Our findings suggested that ROS produced by neutrophils universally reflects the systemic inflammatory condition in cancer patients.


Suzuki K.,Waseda University | Suzuki K.,Tokyo University of Agriculture and Technology | Ohno S.,Waseda University | Ohno S.,Tokyo Women's Medical University | And 11 more authors.
Anticancer Research | Year: 2012

Background/Aim: Oxidative stress in cancer patients has been demonstrated to be partly mediated by neutrophils. Although it is reported that natural antioxidants, such as green tea extract, reduce oxidative stress, there is limited evidence of their effects in cancer patients. This study aimed to determine the effect of green tea extract on reactive oxygen species produced by neutrophils from cancer patients. Materials and Methods: Peripheral blood samples were obtained from eighteen patients with advanced cancer. Green tea extract was added to the blood samples with luminol on Mebiol gel, and luminol-dependent chemiluminescence was measured to monitor the production of reactive oxygen species from migrated neutrophils into the gel, at 37°C. Results: Luminol-dependent chemiluminescence was significantly down-regulated in the presence of green tea extract in a concentration-dependent manner. Conclusion: These results indicate the antioxidant effect of green tea extract on reactive oxygen species produced by neutrophils, which may be effective in reducing oxidative stress in cancer patients.


Sugama K.,Waseda University | Sugama K.,Nihon University | Suzuki K.,Waseda University | Suzuki K.,Tokyo University of Agriculture and Technology | And 6 more authors.
Exercise Immunology Review | Year: 2015

Thioredoxin (TRX) is a 12 kDa protein that is induced by oxidative stress, scavenges reactive oxygen species (ROS) and modulates chemotaxis. Furthermore it is thought to play a protective role in renal ischemia/reperfusion injury. Complement 5a (C5a) is a chemotactic factor of neutrophils and is produced after ischemia/reperfusion injury in the kidney. Both TRX and C5a increase after endurance exercise. Therefore, it may be possible that TRX has an association with C5a in renal disorders and/or renal protection caused by endurance exercise. Accordingly, the aim of this study was to investigate relationships among the changes of urine levels of TRX, C5a and acute kidney injury (AKI) caused by ischemia/reperfusion, inflammatory responses, and oxidative stress following intensive endurance exercise. Also, we applied a newly-developed measurement system of neutrophil migratory activity and ROS-production by use of ex vivo hydrogel methodology with an extracellular matrix to investigate the mechanisms of muscle damage. Fourteen male triathletes participated in a duathlon race consisting of 5 km of running, 40 km of cycling and 5 km of running were recruited to the study. Venous blood and urine samples were collected before, immediately following, 1.5 h and 3 h after the race. Plasma, serum and urine were analyzed using enzyme-linked immunosorbent assays, a free radical analytical system, and the ex vivo neutrophil functional measurement system. These data were analyzed by assigning participants to damaged and minor-damage groups by the presence and absence of renal tubular epithelial cells in the urinary sediments. We found strong associations among urinary TRX, C5a, interleukin (IL)-2, IL-4, IL-8, IL-10, interferon (IFN)-γ and monocyte chemotactic protein (MCP)-1. From the data it might be inferred that urinary TRX, MCP-1 and β-N-acetyl-D-glucosaminidase (NAG) were associated with renal tubular injury. Furthermore, TRX may be influenced by levels of IL-10, regulate chemotactic activity of C5a and IL-8, and control inflammatory progress by C5a and IL-8. In the longer duration group (minor-damage group), circulating neutrophil count, plasma concentration of myeloperoxidase (MPO) and serum concentration of myoglobin were markedly increased. In the higher intensity group (damaged group), neutrophil activation and degranulation of MPO might be inhibited, because not only was ROS production observed to be higher, but also antioxidant capacity and antiinflammatory cytokines were increased. Critically, the newlydeveloped ex vivo methodology corroborated the neutrophil activation levels in the two groups of participants. © 2002 by Hinnak Northoff.


Trademark
Mebiol Inc. | Date: 2011-02-08

Water absorbing polyacrylate resin, artificial plant-cultivating soil containing water absorbing polyacrylate resin, culture growing media for cells or tissues except for medical use, chemicals, namely, industrial chemicals, chemicals for treating hazardous waste, plant growth regulation preparations, adhesives except for office or home use, higher fatty acids for agricultural or industrial purposes, non-metallic minerals, namely, sulphur, huntite, plastic materials, namely, unprocessed plastic materials in powder, liquid or paste form, thermosetting plastic refractory materials, paper and sulphite pulp, flour and starch for industrial purposes, fertilizers, photographic supplies, namely, photographic chemicals, emulsions, chemical test papers, artificial sweeteners, ceramic glazings.


Patent
Mebiol Inc. | Date: 2014-08-11

[Problems] Prior art technology has a problem in that when a plant is cultivated for a long period of time on a polyvinyl alcohol (PVA) film having its lower surface positioned in contact with a nutrient fluid, plant roots that have formed penetrate through the film. [Means to solve the problems] A plant cultivation system wherein the PVA film has an equilibrium degree of swelling in the range of from 125 to 250% as measured in water at 30 C. and has a loss tangent (tan ) in the range of from 0.005 to 0.2 as measured in an equilibrium swollen state in water at 30 C., and a method for cultivating a plant by using this plant cultivation system. [Industrial applicability] Plant cultivation can be performed for a long period of time while avoiding infection by bacteria and the like causative of plant diseases. Therefore, the present invention is useful in, e.g., agriculture and the manufacture of pharmaceuticals.


Patent
Mebiol Inc. | Date: 2016-06-29

[Problems] Prior art technology has a problem in that when a plant is cultivated for a long period of time on a polyvinyl alcohol (PVA) film having its lower surface positioned in contact with a nutrient fluid, plant roots that have formed penetrate through the film. [Means to solve the problems] A plant cultivation system wherein the PVA film has an equilibrium degree of swelling in the range of from 125 to 250 % as measured in water at 30 C and has a loss tangent (tan ) in the range of from 0.005 to 0.2 as measured in an equilibrium swollen state in water at 30 C, and a method for cultivating a plant by using this plant cultivation system. [Industrial applicability] Plant cultivation can be performed for a long period of time while avoiding infection by bacteria and the like causative of plant diseases. Therefore, the present invention is useful in, e.g., agriculture and the manufacture of pharmaceuticals.


PubMed | Ezaki Glico Co., Waseda University, Mebiol Inc. and Kinki University
Type: | Journal: Exercise immunology review | Year: 2015

Thioredoxin (TRX) is a 12 kDa protein that is induced by oxidative stress, scavenges reactive oxygen species (ROS) and modulates chemotaxis. Furthermore it is thought to play a protective role in renal ischemia/reperfusion injury. Complement 5a (C5a) is a chemotactic factor of neutrophils and is produced after ischemia/reperfusion injury in the kidney. Both TRX and C5a increase after endurance exercise. Therefore, it may be possible that TRX has an association with C5a in renal disorders and/or renal protection caused by endurance exercise. Accordingly, the aim of this study was to investigate relationships among the changes of urine levels of TRX, C5a and acute kidney injury (AKI) caused by ischemia/reperfusion, inflammatory responses, and oxidative stress following intensive endurance exercise. Also, we applied a newly-developed measurement system of neutrophil migratory activity and ROS-production by use of ex vivo hydrogel methodology with an extracellular matrix to investigate the mechanisms of muscle damage. Fourteen male triathletes participated in a duathlon race consisting of 5 km of running, 40 km of cycling and 5 km of running were recruited to the study. Venous blood and urine samples were collected before, immediately following, 1.5 h and 3 h after the race. Plasma, serum and urine were analyzed using enzyme-linked immunosorbent assays, a free radical analytical system, and the ex vivo neutrophil functional measurement system. These data were analyzed by assigning participants to damaged and minor-damage groups by the presence and absence of renal tubular epithelial cells in the urinary sediments. We found strong associations among urinary TRX, C5a, interleukin (IL)-2, IL-4, IL-8, IL-10, interferon (IFN)- and monocyte chemotactic protein (MCP)-1. From the data it might be inferred that urinary TRX, MCP-1 and -N-acetyl-D-glucosaminidase (NAG) were associated with renal tubular injury. Furthermore, TRX may be influenced by levels of IL-10, regulate chemotactic activity of C5a and IL-8, and control inflammatory progress by C5a and IL-8. In the longer duration group (minor-damage group), circulating neutrophil count, plasma concentration of myeloperoxidase (MPO) and serum concentration of myoglobin were markedly increased. In the higher intensity group (damaged group), neutrophil activation and degranulation of MPO might be inhibited, because not only was ROS production observed to be higher, but also antioxidant capacity and antiinflammatory cytokines were increased. Critically, the newlydeveloped ex vivo methodology corroborated the neutrophil activation levels in the two groups of participants.


Trademark
Mebiol Inc. | Date: 2010-11-23

[ Chemical fertilizers, natural fertilizers, complexe fertilizers, artificial soil for plant cultivation, plant growth regulating preparations, polymerization plastics; chemicals for use in agriculture, except fungicides, herbicides, insecticides and parasiticides ]. Agricultural implements or machines, namely, seeders, hay balers, cultivators, harvesters, tractor-towed plows and reapers. Plastic films, tubes, or sheeting for agricultural purposes [; semi-worked plastic in the form of pellets, rods, foils, foams, fibers, films and sheets, for use as materials ]. Flower pots; hydroponic apparatus for domestic horticultural purposes, namely, hydroponic garden kit for home use comprising growing containers and also including hydroponic fertilizers, seeds, substrate, and drain components. Frozen vegetables, frozen fruits, processed vegetables * not including vegetable juices for cooking, * processed fruits. Unprocessed edible seaweed, fresh vegetables, fresh fruits, live trees, live grasses, turf, seedlings, live young plants, flowers, potted dwarfed trees in the nature of bonsai.


Trademark
Mebiol Inc. | Date: 2010-11-23

Polymer water absorbent for use in soil for plant cultivation, artificial soil containing water absorbent for plant cultivation, culture media for cells or tissues for scientific and research use, chemicals for use in industry and science, plant growth regulating preparations, adhesives not for household or stationary use, higher fatty acids for the manufacture of soap and laundry detergent; non-metallic minerals, namely, sulphur, huntite, diatomite, dolomite, niter, or bentonite; plastic materials, namely, unprocessed plastic materials in powder, liquid, or paste form; paper pulp; flour and starch for industrial purposes, namely, for use in manufacturing paper and textiles; photographic supplies, namely, photographic chemicals and emulsions; chemical test papers, artificial sweeteners, ceramic glazings. Agricultural implements and machines, namely, seeders, hay balers, cultivators, harvesters, tractor-towed plows and reapers. Plastic films, tubes, or sheeting for agricultural purposes; semi-worked plastic in the form of pellets, rods, foils, foams, fibers, films and sheets, for use as materials. Flower pots; hydroponic apparatus for domestic horticultural purposes, namely, hydroponic garden kit for home use comprising growing containers and also including hydroponic fertilizers, seeds, substrate, and drain components. Frozen vegetables, frozen fruits, processed vegetables, processed fruits. Unprocessed edible seaweed, fresh vegetables, fresh fruits, live trees, live grasses, turf, seedlings, live young plants, flowers, potted dwarfed trees in the nature of bonsai.

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