MBS srl

Rome, Italy
Rome, Italy
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Bottini G.,MBS Srl | Romana Priolisi F.,MBS Srl | Mari A.,MBS Srl | Antonini G.,Third University of Rome | Antonini G.,Italian National Institute of Biosystems and Biostructures
Clinical Chemistry and Laboratory Medicine | Year: 2014

Background: Urinary tract infections (UTIs) are among the most common infectious diseases. Results obtained from conventional microbiological analysis of urine and antibiotic susceptibility testing are available only after a few days, delaying precise diagnosis and appropriate therapy. Micro Biological Survey (MBS) srl (a spin-off of Roma Tre University, Rome, Italy) has developed and patented an automated colorimetric test for rapid bacterial counting. In a preliminary validation study it was demonstrated that the results obtained with the MBS method are equivalent to the results obtained with conventional culture-based microbiological analysis. Methods: In this study, sterile urine samples were artificially contaminated with bacterial species that are most frequently responsible for UTIs. The MBS method was used to evaluate the presence of bacteria and their sensitivity to some of the most commonly used antibiotics in UTIs. Results: The MBS method was able to detect in a few hours the presence or absence of bacteria at clinically significant concentrations ( > 105 CFU/mL), and to provide their susceptibility pattern to a limited panel of antibiotics. Discussion: The results obtained demonstrate that the MBS point-of-care testing (POCT) device could be developed into a valuable aid for the management of UTIs, possibly addressing more precise diagnosis and appropriate therapy.


Losito F.,Third University of Rome | Arienzo A.,Third University of Rome | Bottini G.,MBS S.r.l. | Priolisi F.R.,MBS S.r.l. | And 3 more authors.
Journal of Dairy Science | Year: 2014

Dairy products are characterized by reduced shelf life because they are an excellent growth medium for a wide range of microorganisms. For this reason, it is important to monitor the microbiological quality of dairy products and, in particular, the total viable count and concentration of Escherichia coli, as they are indicators of the hygienic state of these products. In addition, in dairy products such as Mozzarella cheese, it is important to monitor the concentration of lactic acid bacteria (LAB), as they are the major components of starter cultures used in cheese production, contributing to the taste and texture of fermented products and inhibiting food spoilage bacteria by producing growth-inhibiting substances. For these reasons, to ensure the quality and safety of their products, cheese makers should monitor frequently, during fresh cheese production, the concentration of LAB and spoilage bacteria. However, usually, small- to medium-size dairy factories do not have an internal microbiological laboratory and external laboratories of analysis are often too expensive and require several days for the results. Compared with traditional methods, the microbiological survey (MBS) method developed by Roma Tre University (Rome, Italy) allows faster and less-expensive microbiological analyses to be conducted wherever they are necessary, without the need for a microbiological laboratory or any instrumentation other than MBS vials and a thermostat. In this paper, we report the primary validation of the MBS method to monitor LAB concentration in Mozzarella cheese and the analysis, using the MBS method, of total viable count, E. coli, and LAB concentrations in the production line of Mozzarella cheese as well as during the shelf life of the product stored at 20°C. The results obtained indicate that the MBS method may be successfully used by small- to medium-size dairy factories that do not have an internal microbiological laboratory. Using the MBS method, these dairy factories can monitor autonomously the microbiological safety and quality of their products, saving both time and money. © 2014 American Dairy Science Association.


Bottini G.,Third University of Rome | Losito F.S.,Third University of Rome | De Ascentis A.,Third University of Rome | Priolisi F.R.,MBS srl | And 2 more authors.
American Journal of Food Technology | Year: 2011

The aim of this study was the validation of the Micro Biological Survey (MBS) method for microbiological analysis of food for Total Viable Count (TVC) and Escherichia coli (E. coli). The MBS method is a rapid quantitative alternative method for the detection and selective counting of bacteria in agro-food, in water and in environmental samples. It is based on colorimetric survey in mono-use disposable reaction vials that must be filled with the samples without any preliminary treatment (e.g., homogenization, dilution, etc.); the greater the number of bacteria presents into the sample, the faster the color change. However, an independent evaluation of the analytical results obtained with MBS method would be required before commercialization. Therefore, this alternative method was validated in comparison to the reference method. The general estimate of precision, reliability, uncertainty, linearity, accuracy and selectivity were determined. All the performance parameters have demonstrated total correlation between the alternative method and the reference method for the detection and counting of TVC and E. coli both in artificially contaminated and in naturally contaminated samples. MBS assay can be used as rapid and user friendly screening method for detection of TVC and E. coli in food industry. © 2011 Academic Journals Inc.


Losito F.,Third University of Rome | Bottini G.,Third University of Rome | de Ascentis A.,Third University of Rome | Priolisi F.R.,MBS srl | And 3 more authors.
American Journal of Food Technology | Year: 2012

The objective of the present study was the preliminary validation of the qualitative MBS method for Salmonella spp. and Listeria spp. and the quantitative MBS method for Enterobacteriaceae and Staphylococcus aureus. The MBS method is a rapid alternative method for the detection and selective counting of bacteria in agro-food, in water and in environmental samples. It is based on a colorimetric survey in mono-use disposable reaction vials that can be filled with the samples without any preliminary treatment (e.g., homogenization, dilution, etc.); the greater the number of bacteria present in the sample, the faster the color changes. For the qualitative validation of the MBS method for Salmonella spp. and Listeria spp., selectivity, relative accuracy, relative specificity and relative sensitivity were determined. Selectivity, linearity and accuracy were analyzed for the quantitative validation of the MBS method for Enterobacteriaceae and Staphylococcus aureus. The validation here reported has shown that the MBS method for Listeria spp., Salmonella spp., Enterobacteriaceae and S. aureus give similar results and are in agreement with the reference method. © 2012 Academic Journals Inc.


PubMed | MBS S.r.l. and Third University of Rome
Type: Journal Article | Journal: Journal of dairy science | Year: 2013

Dairy products are characterized by reduced shelf life because they are an excellent growth medium for a wide range of microorganisms. For this reason, it is important to monitor the microbiological quality of dairy products and, in particular, the total viable count and concentration of Escherichia coli, as they are indicators of the hygienic state of these products. In addition, in dairy products such as Mozzarella cheese, it is important to monitor the concentration of lactic acid bacteria (LAB), as they are the major components of starter cultures used in cheese production, contributing to the taste and texture of fermented products and inhibiting food spoilage bacteria by producing growth-inhibiting substances. For these reasons, to ensure the quality and safety of their products, cheese makers should monitor frequently, during fresh cheese production, the concentration of LAB and spoilage bacteria. However, usually, small- to medium-size dairy factories do not have an internal microbiological laboratory and external laboratories of analysis are often too expensive and require several days for the results. Compared with traditional methods, the microbiological survey (MBS) method developed by Roma Tre University (Rome, Italy) allows faster and less-expensive microbiological analyses to be conducted wherever they are necessary, without the need for a microbiological laboratory or any instrumentation other than MBS vials and a thermostat. In this paper, we report the primary validation of the MBS method to monitor LAB concentration in Mozzarella cheese and the analysis, using the MBS method, of total viable count, E. coli, and LAB concentrations in the production line of Mozzarella cheese as well as during the shelf life of the product stored at 20C. The results obtained indicate that the MBS method may be successfully used by small- to medium-size dairy factories that do not have an internal microbiological laboratory. Using the MBS method, these dairy factories can monitor autonomously the microbiological safety and quality of their products, saving both time and money.

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