Mazatlán, Mexico
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Leon Robles A.,Guaymas Unit Quality Assurance and Management of Natural Resources | Acedo Felix E.,CIAD | Gomez-Gil B.,Mazatlan Unit for Aquaculture | Quinones Ramirez E.I.,Laboratory Of Microbiologia Sanitaria | And 2 more authors.
Journal of Water and Health | Year: 2013

Members of the genus Vibrio are common in aquatic environments. Among them are V. cholerae, V. vulnificus, V. parahaemolyticus and V. mimicus. Several studies have shown that environmental factors, such as temperature, salinity, and dissolved oxygen, are involved in their epidemiology. Therefore, the main objective of this study is to determine if there is a correlation between the presence/amount of V. cholerae, V, vulnificus, V. parahaemolyticus and V. mimicus and the environmental conditions of the seawater off the coast of Guaymas, México. Quantification of all four pathogenic bacteria was performed using the most probable number method, and suspected colonies were identified by polymerase chain reaction (PCR). Correlations were found using principal component analysis. V. parahaemolyticus was the most abundant and widely distributed bacteria, followed by V. vulnificus, V. mimicus and V. cholerae. Positive correlations between V. parahaemolyticus, V. vulnificus and V. mimicus with temperature, salinity, electric conductivity, and total dissolved solids were found. The abundance of V. cholerae was mainly affected by the sampling site and not by physicochemical parameters. © IWA Publishing 2013.


Lin B.,Center for Bio Molecular Science and Engineering | Wang Z.,Center for Bio Molecular Science and Engineering | Malanoski A.P.,Center for Bio Molecular Science and Engineering | O'Grady E.A.,University of Wisconsin - Milwaukee | And 6 more authors.
Environmental Microbiology Reports | Year: 2010

Three notable members of the Harveyi clade, Vibrio harveyi, Vibrio alginolyticus and Vibrio parahaemolyticus, are best known as marine pathogens of commercial and medical import. In spite of this fact, the discrimination of Harveyi clade members remains difficult due to genetic and phenotypic similarities, and this has led to misidentifications and inaccurate estimations of a species' involvement in certain environments. To begin to understand the underlying genetics that complicate species level discrimination, we compared the genomes of Harveyi clade members isolated from different environments (seawater, shrimp, corals, oysters, finfish, humans) using microarray-based comparative genomic hybridization(CGH) and multilocus sequence analyses (MLSA). Surprisingly, we found that the only two V. harveyi strains that have had their genomes sequenced (strains BAA-1116 and HY01) have themselves been misidentified. Instead of belonging to the species harveyi, they are actually members of the species campbellii. In total, 28% of the strains tested were found to be misidentified and 42% of these appear to comprise a novel species. Taken together, our findings correct a number of species misidentifications while validating the ability of both CGH and MLSA to distinguish closely related members of the Harveyi clade. © 2009 Society for Applied Microbiology and Blackwell Publishing Ltd.


Espinoza-Valles I.,Mazatlan Unit for Aquaculture | Vora G.J.,2 Center for Bio Molecular Science & Engineering | Lin B.,2 Center for Bio Molecular Science & Engineering | Leekitcharoenphon P.,Technical University of Denmark | And 4 more authors.
Microbiology (Reading, England) | Year: 2015

Vibrio harveyi CAIM 1792 is a marine bacterial strain that causes mortality in farmed shrimp in north-west Mexico, and the identification of virulence genes in this strain is important for understanding its pathogenicity. The aim of this work was to compare the V. harveyi CAIM 1792 genome with related genome sequences to determine their phylogenic relationship and explore unique regions in silico that differentiate this strain from other V. harveyi strains. Twenty-one newly sequenced genomes were compared in silico against the CAIM 1792 genome at nucleotidic and predicted proteome levels. The proteome of CAIM 1792 had higher similarity to those of other V. harveyi strains (78%) than to those of the other closely related species Vibrio owensii (67%), Vibrio rotiferianus (63%) and Vibrio campbellii (59%). Pan-genome ORFans trees showed the best fit with the accepted phylogeny based on DNA-DNA hybridization and multi-locus sequence analysis of 11 concatenated housekeeping genes. SNP analysis clustered 34/38 genomes within their accepted species. The pangenomic and SNP trees showed that V. harveyi is the most conserved of the four species studied and V. campbellii may be divided into at least three subspecies, supported by intergenomic distance analysis. blastp atlases were created to identify unique regions among the genomes most related to V. harveyi CAIM 1792; these regions included genes encoding glycosyltransferases, specific type restriction modification systems and a transcriptional regulator, LysR, reported to be involved in virulence, metabolism, quorum sensing and motility.


Grano-Maldonado M.,National Autonomous University of Mexico | Grano-Maldonado M.,Mazatlan Unit for Aquaculture | Roque A.,Institute of Research and Technology in Food and Agriculture | Fajer-Avila E.J.,Mazatlan Unit for Aquaculture
Fish Pathology | Year: 2010

Heterobothrium ecuadori is a gill monogenean infecting the gills of the bullseye puffer fish Sphoeroides annulatus and feeding fish blood. This experimental study was performed to describe the juvenile stages of H. ecuadori and to determine the time required to reach the adult stage at 20-23°C. Uninfected bullseye puffer fish were exposed to oncomiracidia and sampled at intervals to obtain worms of different developmental stages. Between 5 and 10 days after the exposure, the first and second pairs of clamps were observed in worms along with the emergence of the third clamp. Four pairs of clamps were observed on day 15, and primordial male copulatory organ, testes and ovary were found on day 20. Adult parasites started to appear between 25 and 30 days post-infection. The time required for egg laying was estimated to be 33 days, based on the first collection of eggs in the rearing water. © 2010 The Japanese Society of Fish Pathology.


Luis-Villasenor I.E.,National Polytechnic Institute of Mexico | Castellanos-Cervantes T.,National Polytechnic Institute of Mexico | Gomez-Gil B.,Mazatlan Unit for Aquaculture | Carrillo-Garcia A.E.,National Polytechnic Institute of Mexico | And 2 more authors.
World Journal of Microbiology and Biotechnology | Year: 2013

Molecular analysis of the 16S rDNA of the intestinal microbiota of whiteleg shrimp Litopenaeus vannamei was examined to investigate the effect of a Bacillus mix (Bacillus endophyticus YC3-b, Bacillus endophyticus C2-2, Bacillus tequilensisYC5-2) and the commercial probiotic (Alibio®) on intestinal bacterial communities and resistance to Vibrio infection. PCR and single strain conformation polymorphism (SSCP) analyses were then performed on DNA extracted directly from guts. Injection of shrimp with V. parahaemolyticus at 2. 5 × 105 CFU g-1 per shrimp followed 168 h after inoculation with Bacillus mix or the Alibio probiotic or the positive control. Diversity analyses showed that the bacterial community resulting from the Bacillus mix had the highest diversity and evenness and the bacterial community of the control had the lowest diversity. The bacterial community treated with probiotics mainly consisted of α- and γ-proteobacteria, fusobacteria, sphingobacteria, and flavobacteria, while the control mainly consisted of α-proteobacteria and flavobacteria. Differences were grouped using principal component analyses of PCR-SSCP of the microbiota, according to the time of inoculation. In Vibrio parahaemolyticus-infected shrimp, the Bacillus mix (~33 %) induced a significant increase in survival compared to Alibio (~21 %) and the control (~9 %). We conclude that administration of the Bacillus mix induced modulation of the intestinal microbiota of L. vannamei and increased its resistance to V. parahaemolyticus. © 2012 Springer Science+Business Media Dordrecht.


Gonzalez-Castillo A.,Mazatlan Unit for Aquaculture | Enciso-Ibarrra J.,Mazatlan Unit for Aquaculture | Bolan-Mejia M.C.,Mazatlan Unit for Aquaculture | Balboa S.,University of Santiago de Compostela | And 4 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2015

A bacterial strain was taxonomically characterised by means of a genomic approach comprising 16S rRNA gene sequence analysis, multilocus sequence analysis (MLSA), theDNAG+Ccontent,whole genome analyses (ANI and GGDC) and phenotypic characterisation. The strainCAIM1540T was isolated from a cultured oyster Crassostrea corteziensis in La Cruz, Sinaloa state, Me´xico. The isolatewas found to be catalase and oxidase positive, cells were observed to be motile, O/129-sensitive and facultatively anaerobic. The almost-complete 16S rRNA gene sequence placed this strain within the genus Vibrio; the closest related specieswere found to be Vibrio aestivus, Vibrio marisflavi, Vibrio maritimus and Vibrio variabilis with similarity values of 99.02, 97.05, 96.70, and 96.59 % respectively. MLSA of four housekeeping genes (ftsZ, gapA, recA, and topA) was performed with the closely related species. A draft genome sequence of strain CAIM 1540T was obtained. The DNA G+C content of this strain was determined to be 43.7 mol%.The ANI values with V. aestivus were 89.6 % (ANIb), 90.6 % (ANIm) and a GGDC value of 39.5 ± 2.5 % was obtained; with V. marisflavi the genomic similarities were 71.5 % (ANIb), 85.5 % (ANIm) and 20.2 ± 2.3 % (GGDC); with V. maritimus 72.6 % (ANIb), 85.7 % (ANIm) and 22.0 ± 2.0 % (GGDC); and with V. variabilis 72.6 % (ANIb), 85.8 % (ANIm) and 21.6 ± 1.6 % (GGDC). These ANI and GGDC values are below the threshold for the delimitation of prokaryotic species, i.e. 95–96 and 70 %, respectively. Phenotypic characters also showed differences with the closest related species analysed. The results presented here support the description of a novel species, for which the name Vibrio mexicanus sp. nov. is proposed, with strain CAIM 1540T (= CECT 8828T, = DSM 100338T) as the type strain. In addition, we found that the recently described species Vibrio thalassae and Vibrio madracius might be a single species because the values of ANIb 95.8 %, ANIm 96.6 % and GGDC 70.2 ± 2.9 %are above the accepted species thresholds. © Springer International Publishing Switzerland 2015.


PubMed | University of Santiago de Compostela and Mazatlan Unit for Aquaculture
Type: Journal Article | Journal: Systematic and applied microbiology | Year: 2016

Seven isolates were obtained from different culture stages of carpet shell clam (Ruditapes decussatus) reared in a bivalve hatchery (Galicia, NW Spain). Three groups were differentiated by genotyping techniques and phenotypic profiles and representative trains were selected to further taxonomic studies. These strains were studied by a polyphasic approach and in basis of the phylogenetic analysis based on concatenated sequences of the five housekeeping genes ftsZ, gyrB, pyrH, recA and rpoA formed a tight group into the Mediterranei clade of the genus Vibrio. Percentages of genomic resemblance, including average nucleotide identity, in silico genome-to-genome comparison and wet DNA-DNA hybridization between the type strain and the closest relatives Vibrio mediterranei and Vibrio thalassae were below of the proposed boundaries for the definition of species. The novel isolates could be also differentiated from the related taxa on the basis of several phenotypic traits and fatty acid profiles. Results obtained support the description of a novel species into the Mediterranei clade, for which the name Vibrio barjaei sp. nov. is proposed, with strain 3062


PubMed | University of Santiago de Compostela and Mazatlan Unit for Aquaculture
Type: Journal Article | Journal: Antonie van Leeuwenhoek | Year: 2016

Strain CAIM 1076


PubMed | Mazatlan Unit for Aquaculture
Type: Journal Article | Journal: Antonie van Leeuwenhoek | Year: 2015

A bacterial strain was taxonomically characterised by means of a genomic approach comprising 16S rRNA gene sequence analysis, multilocus sequence analysis (MLSA), the DNA G+C content, whole genome analyses (ANI and GGDC) and phenotypic characterisation. The strain CAIM 1540(T) was isolated from a cultured oyster Crassostrea corteziensis in La Cruz, Sinaloa state, Mxico. The isolate was found to be catalase and oxidase positive, cells were observed to be motile, O/129-sensitive and facultatively anaerobic. The almost-complete 16S rRNA gene sequence placed this strain within the genus Vibrio; the closest related species were found to be Vibrio aestivus, Vibrio marisflavi, Vibrio maritimus and Vibrio variabilis with similarity values of 99.02, 97.05, 96.70, and 96.59 % respectively. MLSA of four housekeeping genes (ftsZ, gapA, recA, and topA) was performed with the closely related species. A draft genome sequence of strain CAIM 1540(T) was obtained. The DNA G+C content of this strain was determined to be 43.7 mol%.The ANI values with V. aestivus were 89.6 % (ANIb), 90.6 % (ANIm) and a GGDC value of 39.5 2.5 % was obtained; with V. marisflavi the genomic similarities were 71.5 % (ANIb), 85.5 % (ANIm) and 20.2 2.3 % (GGDC); with V. maritimus 72.6 % (ANIb), 85.7 % (ANIm) and 22.0 2.0 % (GGDC); and with V. variabilis 72.6 % (ANIb), 85.8 % (ANIm) and 21.6 1.6 % (GGDC). These ANI and GGDC values are below the threshold for the delimitation of prokaryotic species, i.e. 95-96 and 70 %, respectively. Phenotypic characters also showed differences with the closest related species analysed. The results presented here support the description of a novel species, for which the name Vibrio mexicanus sp. nov. is proposed, with strain CAIM 1540(T) (= CECT 8828(T), = DSM 100338(T)) as the type strain. In addition, we found that the recently described species Vibrio thalassae and Vibrio madracius might be a single species because the values of ANIb 95.8 %, ANIm 96.6 % and GGDC 70.2 2.9 % are above the accepted species thresholds.


Gonzalez-Castillo A.,Mazatlan Unit for Aquaculture | Enciso-Ibarra J.,Mazatlan Unit for Aquaculture | Dubert J.,University of Santiago de Compostela | Romalde J.L.,University of Santiago de Compostela | Gomez-Gil B.,Mazatlan Unit for Aquaculture
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2016

Strain CAIM 1076T was isolated from a cultured oyster Crassostrea gigas in Puerto Peñasco, Sonora state, México. The strain was taxonomically characterised by means of a genomic approach, comprising 16S rRNA gene sequence analysis, multilocus sequence analysis (MLSA), the DNA G+C content and whole genome analyses (ANI and GGDC), and by phenotypic characterisation. Strain CAIM 1076T was found to be catalase and oxidase positive, and cells were observed to be motile and facultative anaerobic. Analysis of the almost-complete 16S rRNA gene sequence placed this strain within the genus Vibrio; closely related species were Vibrio maritimus, Vibrio variabilis, Vibrioproteolyticus, and Vibrio nigripulchritudo with similarity values of 98.9, 98.5, 98.1, and 98.0 %, respectively. MLSA of six housekeeping genes (ftsZ, gapA, gyrB, recA, rpoA and topA) was performed with the closely related species. A draft genome sequence of strain CAIM 1076T was obtained. The DNA G+C content of this strain was determined to be 44.5 mol %. The genomic similarity values with V. maritimus were 71.6 % (ANIb), 85.1 % (ANIm) and a GGDC value of 20.3 ± 2.3 %; with V. variabilis the genomic similarities were 71.8 % (ANIb), 85.4 % (ANIm) and 20.0 ± 2.3 % (GGDC); with V.proteolyticus, 71.6 % (ANIb), 84.1 % (ANIm) and 18.8 ± 2.2 % (GGDC); and with V. nigripulchritudo, 70.8 % (ANIb), 84.9 % (ANIm) and 20.5 ± 2.3 % (GGDC). These ANI and GGDC values are below the thresholds for the delimitation of prokaryotic species, i.e., 95–96 and 70 %, respectively. Phenotypic characters also showed differences with the closely related species analysed. The results presented here support the description of a novel species, for which the name Vibrio sonorensis sp. nov. is proposed, with strain CAIM 1076T (=CECT 9100T, =DSM 102190T) as the type strain. © 2016 Springer International Publishing Switzerland

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