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Rochester, MN, United States

Zhang B.,University of Minnesota | Iezzi R.,MayoClinic | Cui T.,University of Minnesota
2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 | Year: 2013

The monitoring and controlling of vascular endothelial growth factor (VEGC) are very impnrtant in the treatment of certain cancers and age-related macular degeneration. Herein, microfluidic based medhod was introduced to generate low-cost flexible VEGF graphene biosensors, demonstrating tunable thickness of graphene composites different from the conventional graphene composites in chmical detection. After alternating the surface wetting ability, a PDMS based microfluidic system duplicated from a silicon mold was used to introduce, confine and pattern the graphene films. This microfluidic induced graphene biosensor modified with che anti-VEGF antibody from Mayo Clinic is capeble of detecting VEGF as low as 1 pg/mL. A promising technique be presented to develop the low-cost and high-performance biosensors for the VEGF detection. © 2013 IEEE.

Karp D.D.,University of Houston | Lee S.J.,Dana-Farber Cancer Institute | Keller S.M.,Montefiore Medical Center | Wright G.S.,Florida Cancer Specialists | And 15 more authors.
Journal of Clinical Oncology | Year: 2013

Purpose Selenium has been reported to have chemopreventive benefits in lung cancer. We conducted a double-blind, placebo-controlled trial to evaluate the incidence of second primary tumors (SPTs) in patients with resected non-small-cell lung cancer (NSCLC) receiving selenium supplementation. Patients and Methods Patients with completely resected stage I NSCLC were randomly assigned to take selenized yeast 200

Mathison A.,Chromatin | Grzenda A.,Chromatin | Lomberk G.,Chromatin | Velez G.,Chromatin | And 11 more authors.
PLoS ONE | Year: 2013

Krüppel-like factor 11 (KLF11) and the highly homologous KLF10 proteins are transcription factors originating from duplication of the Drosophila melanogaster ancestor cabut. The function of these proteins in epithelial cells has been previously characterized. In the current study, we report a functional role for KLF11 in mesenchymal cells and in mesenchymal cell dysfunction, namely, fibrosis, and subsequently perform a detailed cellular, molecular, and in vivo characterization of this phenomenon. We find that, in cultured mesenchymal cells, enhanced expression of KLF11 results in activated extracellular matrix pathways, including collagen gene silencing and matrix metalloproteinases activation without changes in tissue inhibitors of metalloproteinases. Combined, reporter and chromatin immunoprecipitation assays demonstrate that KLF11 interacts directly with the collagen 1a2 (COL1A2) promoter in mesenchymal cells to repress its activity. Mechanistically, KLF11 regulates collagen gene expression through the heterochromatin protein 1 gene-silencing pathway as mutants defective for coupling to this epigenetic modifier lose the ability to repress COL1A2. Expression studies reveal decreased levels of KLF11 during liver fibrogenesis after chemically induced injury in vivo. Congruently, KLF11-/- mice, which should be deficient in the hypothesized anti-fibrogenic brake imposed by this transcription factor, display an enhanced response to liver injury with increased collagen fibril deposition. Thus, KLFs expands the repertoire of transcription factors involved in the regulation of extracellular matrix proteins in mesenchymal cells and define a novel pathway that modulates the fibrogenic response during liver injury. © 2013 Mathison et al.

Van Doesburg M.H.M.,University Utrecht | Van Der Molen A.M.,University Utrecht | Cha S.S.,MayoClinic
Journal of Ultrasound in Medicine | Year: 2012

Objectives-Amajor pathologic finding in patients with idiopathic carpal tunnel syndrome is noninflammatory fibrosis and thickening of the subsynovial connective tissue. The objective of this study was to determine the ability of sonography to depict this thickening by comparing subsynovial connective tissue thickness in patients with carpal tunnel syndrome and healthy control participants. Methods-Longitudinal sonograms of the middle finger superficial flexor tendon and subsynovial connective tissue were obtained at 3 levels: at the wrist crease (proximal tunnel), at the hook of the hamate (mid tunnel), and at the distal edge of the transverse carpal ligament (distal tunnel). The thickness of the subsynovial connective tissue perpendicular to the direction of the tendon and the diameter of the flexor digitorum superficialis tendon at the same level were measured. Then, a thickness ratio was created. Results-At all 3 levels, the subsynovial connective tissue was thicker in patients than in controls (P < .0001) with a thickness ranging from 0.60 to 0.63 mm in patients and 0.46 to 0.50 mm in controls. The thickness ratio was significantly greater in patients at the hamate and distal levels (P = .018 and .013, respectively). Conclusions-With this study, we have shown that it is possible to measure subsynovial connective tissue thickness with sonography, and the tissue is thicker in patients with carpal tunnel syndrome than in healthy controls. © 2012 by the American Institute of Ultrasound in Medicine.

Spoon D.B.,MayoClinic | Orszulak T.A.,MayoClinic | Edell E.S.,MayoClinic | Li Z.,MayoClinic | Nishimura R.A.,MayoClinic
Journal of Heart Valve Disease | Year: 2012

Background and aim of the study: Patients with aortic stenosis (AS) and chronic obstructive pulmonary disease (COPD) have been considered at high risk for aortic valve replacement (AVR), which results in some patients being denied this life-saving operation. Hence, the study aim was to assess the operative, 30-day, and long-term mortality in individuals with COPD undergoing AVR for AS in the modern surgical era. Methods: This retrospective cohort of patients had documented COPD (FEV1/FVC <70%), and underwent isolated AVR for severe AS between 1993 and 2007 at the Mayo Clinic in Rochester, MN. Results: Of the 68 patients who met the study criteria, 27 had mild/moderate COPD (FEV 1 >50%), 35 had severe COPD (FEV1 30-50%), and six had very severe COPD (FEV1 <30%). The overall operative and 30-day mortality was 4.8%. More severe COPD was associated with a longer stay in the intensive care unit (42 h for mild/moderate versus 115 h for severe/very severe: p = 0.02), but did not influence the operative or 30-day mortalities. Female gender was associated with an increased length of hospital stay. Long-term mortality was significantly higher in patients with a history of cerebrovascular disease (HR 4.3, p <0.001), NYHA class III or IV heart failure (class III HR 2.79, p = 0.05; class IV HR 3.97, p = 0.03), and increased age (HR 1.06, p = 0.003). The severity of COPD was an independent risk factor for long-term mortality. Conclusion: Patients with severe AS and COPD are at an acceptable risk for AVR (30-day mortality <5%). The severity of COPD is not associated with an increased in-hospital or 30-day mortality, but does influence long-term mortality. © Copyright by ICR Publishers 2012.

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