Sporbert A.,Max Delbrueck Center for Molecular Medicine Berlin |
Cseresnyes Z.,Max Delbrueck Center for Molecular Medicine Berlin |
Heidbreder M.,University of Würzburg |
Domaing P.,Max Delbrueck Center for Molecular Medicine Berlin |
And 5 more authors.
PLoS ONE | Year: 2013
This study describes a simple technique that improves a recently developed 3D sub-diffraction imaging method based on three-photon absorption of commercially available quantum dots. The method combines imaging of biological samples via tri-exciton generation in quantum dots with deconvolution and spectral multiplexing, resulting in a novel approach for multi-color imaging of even thick biological samples at a 1.4 to 1.9-fold better spatial resolution. This approach is realized on a conventional confocal microscope equipped with standard continuous-wave lasers. We demonstrate the potential of multi-color tri-exciton imaging of quantum dots combined with deconvolution on viral vesicles in lentivirally transduced cells as well as intermediate filaments in three-dimensional clusters of mouse-derived neural stem cells (neurospheres) and dense microtubuli arrays in myotubes formed by stacks of differentiated C2C12 myoblasts. © 2013 Sporbert et al.