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Queen Victoria, Mauritius

Ramanjooloo A.,Mauritius Oceanography Institute MOI | Beedessee G.,Mauritius Oceanography Institute MOI | Arya D.,National Center for Biological science | VanSoest R.W.M.,Netherlands Center for Biodiversity Naturalis | And 2 more authors.
Natural Product Communications | Year: 2013

As part of our ongoing studies on bioactive natural products from marine sponges, we investigated the cytotoxic potential of extracts from the new sponge Petrosia tuberosa sampled from Mauritius waters. Bioguided fractionation of the ethyl acetate extract by vacuum liquid chromatography (VLC) revealed two fractions, namely VLC (6-9) and (13-17) showing cell deaths of 86 ± 1% and 88 ± 4%, respectively, at 50 μg/mL on HeLa cells. At 10 μg/mL, only VLC (13-17) displayed a significant cell death (56 ± 7%) compared with VLC (6-9) (8 ± 1%). The cytotoxic activity of VLC (13-17) was also determined on nine other human cancer cell lines. Clonogenic assay, mitochondrial membrane potential change, DNA fragmentation and microscopic analysis of fraction VLC (13-17) revealed distinct features of apoptosis on HeLa cells. Further fractionation and purification of this fraction by chromatographic techniques resulted in isolation of one known secondary metabolite, petrosynol. Its structure was determined by 1H and 13C-NMR analyses.

Beedessee G.,Mauritius Oceanography Institute MOI | Ramanjooloo A.,Mauritius Oceanography Institute MOI | Tiscornia I.,Institute Pasteur Of Montevideo Ipmon | Cresteil T.,CNRS Natural Product Chemistry Institute | And 7 more authors.
Journal of Pharmacy and Pharmacology | Year: 2014

Objectives Based on previous screening results, the cytotoxic effect of the hexane (JDH) and ethyl acetate extracts (JDE) of the marine sponge Jaspis diastra were evaluated on HeLa cells and the present study aimed at determining their possible mechanism of cell death. Methods Nuclear staining, membrane potential change, flow cytometry analysis of cell cycle distribution and annexin V staining were undertaken to investigate the effects of JDE and JDH. Electrospray ionization mass spectrometry (ESI-MS) and nuclear magnetic resonance were used to characterize an isolated bioactive molecule. Key findings JDE displayed an IC50 25 times more significant than the JDH. Flow cytometry analysis revealed JDE induced apoptosis in HeLa cells accompanied by the collapse of mitochondrial membrane potential. Fractionation of JDE resulted in the isolation of the known cytotoxic cyclodepsipeptide, Jaspamide. Conclusions Taking our results together suggest that JDE can be valuable for the development of anticancer drugs, especially for cervical cancer. Further investigations are currently in progress with the aim to determine and isolate other bioactive compounds from this extract. © 2014 Royal Pharmaceutical Society.

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