A high-performance thin-layer chromatographic method for the evaluation of marmelosin from aegle marmelos corr. Extract and its traditional formulation from rat plasma: Application to pharmacokinetics
Shailajan S.,Matunga East Herbal Research Laboratory |
Hande H.,Matunga East Herbal Research Laboratory |
Joshi H.,Matunga East Herbal Research Laboratory |
Tiwari B.,Matunga East Herbal Research Laboratory |
Menon S.,Institute for Advanced Training and Research in Interdisciplinary science 194
Journal of Planar Chromatography - Modern TLC | Year: 2014
Marmelosin is one of the major phytochemical constituents of Aegle marmelos (L.) Corr. (Rutaceae). Till date, there are no methods reported for the estimation of marmelosin in plasma matrix using high-performance thin-layer chromatography (HPTLC) which is a versatile analytical technique promoting shorter analysis time and less consumption of solvents with efficient data acquisition and processing. This study is an attempt to develop a rapid and sensitive HPTLC method for the estimation of marmelosin from rat plasma and its application to evaluate pharmacokinetics of A. marmelos fruit pulp extract and a traditional Unani medicine Sharabat-e-Belgiri. Plasma processing involved liquid-liquid extraction with diethyl ether followed by HPTLC. Separation and determination of marmelosin were performed on silica gel 60 F25 TLC plate using toluene-ethyl acetate-glacial acetic acid as mobile phase. United States Food and Drug Administration (USFDA) guidelines for bioanalytical validation were followed to validate the HPTLC method. RF of marmelosin was found to be 0.57. The calibration curve was linear over the range of 1.0-150.0 μg mL-1 (r2 = 0.995) in rat plasma. Limit of detection and limit of quantitation were found to be 0.5 μg mL -1 and 1.0 μg mL-1, respectively. The extraction recovery from plasma was in the range of 90.07-98.22%. The intra-day and inter-day precisions were found to be 90.89-104.12%, and 92.82-104.86%, respectively. The absorption of marmelosin from extract was found significantly higher than the formulation. The results indicated that HPTLC is a suitable technique for the estimation and pharmacokinetic evaluation of marmelosin from plant extract and Unani formulation in rat plasma.