Kanda N.,Kyoto University |
Morimoto N.,Kyoto University |
Takemoto S.,Matsue City Hospital |
Ayvazyan A.A.,Kyoto University |
And 4 more authors.
Annals of Plastic Surgery | Year: 2012
We have developed collagen/gelatin sponges (CGS) with a gelatin concentration of 10 wt% to sustain the release of basic fibroblast growth factor (bFGF). The objective of this study is to elucidate the efficacy of CGS impregnated with different concentrations of bFGF, using mouse skin defects. CGSs impregnated with normal saline solution (NSS) or bFGF solution (1, 7, 14, or 50 μg/cm) were implanted into full-thickness skin defects on the backs of mice. The wound area, neoepithelium length, and total area of newly formed capillaries in CGS were evaluated. The group of CGS with 7-μg/cm bFGF was significantly superior to the NSS group in all evaluated items. CGS impregnated with the appropriate dosage of bFGF accelerates dermis-like tissue formation 2 or 3 times earlier than existing artificial dermis. The combination of CGS and bFGF could solve the problem of the existing artificial dermis and be very promising for the treatment of skin defects. Copyright © 2012 Lippincott Williams &Wilkins.
Itamochi H.,Tottori University |
Oumi N.,Tottori University |
Oishi T.,Tottori University |
Shoji T.,Iwate Medical University |
And 5 more authors.
International Journal of Clinical Oncology | Year: 2015
Background: Recent studies have shown that somatic mutations in the AT-rich interactive domain 1A (SWI-like) gene (ARID1A) are the most common genetic changes in clear cell carcinoma of the ovary (CCC). A gene mutation of ARID1A was found in approximately half of CCC cases, and led to absence of the encoded protein and inactivation of the putative tumor suppressor. Here, we investigated whether ARID1A could be a prognostic biomarker for this disease. Methods: We analyzed the protein expression of ARID1A in CCC from 112 patients by immunohistochemical staining, and evaluated the association of these molecular parameters with clinical outcome. Results: The loss of ARID1A expression was found in 39 % (44/112) of CCC, and was not associated with patient age, FIGO stage, and status of residual tumor. The 5-year survival rate for FIGO stage I or II patients with negative tumor expression of ARID1A was lower than those with positive tumor expression of ARID1A (74 % vs 91 %), but this difference was not observed in FIGO stage III or IV patients. Multivariable analysis revealed that ARID1A expression was an independent prognostic factor in FIGO stage I or II CCC patients. Conclusion: ARID1A may be a biomarker that is predictive of the outcome of FIGO stage I and II CCC. © 2015, Japan Society of Clinical Oncology.
Shimura S.,The University of Shimane |
Ishimura N.,The University of Shimane |
Tanimura T.,Matsue City Hospital |
Yuki T.,The University of Shimane |
And 10 more authors.
Digestion | Year: 2014
Background/Aims: The clinical characteristics of esophageal eosinophilia (EE), which is essential for diagnosis of eosinophilic esophagitis (EoE), have not been fully clarified in a Japanese population. The aim of this study was to analyze the reliability of symptoms and endoscopic findings for diagnosing EE in Japanese individuals. Methods: We prospectively enrolled subjects who complained of esophageal symptoms suggesting EoE and/or those with endoscopic findings of suspected EoE at the outpatient clinics of 12 hospitals. Diagnostic utility was compared between the EE and non-EE groups using logistic regression analysis. Results: A total of 349 patients, including 319 with symptoms and 30 with no symptoms but endoscopic findings suggesting EoE were enrolled. Of those with symptoms, 8 (2.5%) had EE, and 3 were finally diagnosed with EoE. Of those without symptoms but endoscopic findings, 4 had EE. Among 8 symptomatic patients, 7 had abnormal endoscopic findings suspicious of EoE. Although dysphagia was a major symptom in EE, none of the presenting symptoms was useful for diagnosis of EE. Among the endoscopic findings, linear furrow was the most reliable (OR = 41.583). Conclusion: EE is uncommon among patients with esophageal symptoms in Japanese individuals. The most useful endoscopic finding for diagnosis of EE was linear furrow, whereas subjective symptoms were not supportive. © 2014 S. Karger AG, Basel.
Mizumoto K.,Matsue City Hospital
Nishinihon Journal of Dermatology | Year: 2011
A 74-year-old woman had high fever, over 39°C, and itchy constant dark violet eruptions on her left upper arm and back. Her plasma level of ferritin was high. After further examination, she was diagnosed with adult Still's disease because her plasma level of interleukin (IL)-18 was notably high, and she was administered prednisolone at a daily dose of 30 mg. During the course of the disease, high fever recurred and she was administered a daily dose of 40 mg of prednisolone, which rapidly reduced the fever. After that the daily dose of prednisolone was gradually decreased to a daily dose of 5 mg. and she have had no recurrence through the 70th day after her first visit. Although this case did not fulfill Yamaguchi's diagnostic criteria of the adult Still's disease, with recent knowledge we were able to treat it rapidly because of the examination of the plasma level of IL-18. We considered the examination of inflammatory cytokines, such as IL-18, useful for the diagnosis of adult Still's disease.
Nariai Y.,Matsue City Hospital |
Nariai Y.,The University of Shimane |
Kanno T.,The University of Shimane |
Sekine J.,The University of Shimane
Journal of Oral and Maxillofacial Surgery | Year: 2016
Oral squamous cell carcinoma (OSCC) around a dental implant is a rare pathologic condition. This report describes a case of recurrent OSCC surrounding a dental implant, histopathologic findings, and a literature review of this condition. A 58-year-old Japanese woman underwent chemoradiotherapy for OSCC in the right lower gingiva, resulting in a complete response. Nine years after primary chemoradiotherapy, a dental implant was placed in her atrophic mandible. Three years later, an OSCC developed around the dental implant in the right lower premolar region. Marginal mandibulectomy was performed. Microscopic examination showed medullary invasion around the implant surface, suggesting that tumor infiltration of the bone was through the interface between the implant and bone. However, no downward invasion through the interface was evident. OSCC can develop around dental implants that are placed for oral rehabilitation after ablative surgery. Staging of OSCC and planning of surgical management should be carried out carefully, because implants placed adjacent to the OSCC can influence tumor invasion. A high degree of vigilance for OSCC is required during follow-up of patients with dental implants who have OSCC risk factors such as premalignant lesions. Detailed evaluation, including biopsy examination, is essential for distinguishing peri-implantitis from OSCC. © 2016 American Association of Oral and Maxillofacial Surgeons.