Maternity and Child Care Hospital

Taiyuan, China

Maternity and Child Care Hospital

Taiyuan, China
SEARCH FILTERS
Time filter
Source Type

Liu Z.,Shandong University | Chen X.,Shandong University | Wu Q.,Maternity and Child Care Hospital | Song J.,Shandong University | And 2 more authors.
European Journal of Pharmacology | Year: 2016

Asthma is a disease characterized by goblet cell differentiation, mucus hypersecretion, airway inflammation, and airway hyperresponsiveness. miR-125b was downregulated as normal human bronchial epithelial cells differentiation to pseudostratified epithelium. However, its role in asthma remains unknown especially in regulating goblet cell differentiation. miR-125b expression in the sputum of 50 asthmatic children and 50 age- and sex-matched healthy controls were assessed by quantitative RT-PCR (qRT-PCR). Meanwhile, expressions of miR-125b and SAM pointed domain-containing ETS transcription factor (SPDEF) in normal human tracheal epithelial (HTEpC) and A549 cells stimulated with lipopolysaccharide (LPS) for 2 h were detected by qRT-PCR and western blot. Furthermore, the predicted miR-125b target was determined in silico and confirmed with dual-luciferase reporter assay. Additionally, intranasal delivery of miR-125b mimic in mice was performed to study its effects on house dust mite-induced allergic airway inflammation mouse models. We found that miR-125b expression was decreased in the sputum of the asthmatic patients especially in eosinophilic asthma. After stimulation with LPS, miR-125b expression was downregulated, accompanied by the upregulation of SPDEF in HTEpC and A549 cells. Moreover, SPDEF is a target of miR-125b, which regulates SPDEF at the posttranscriptional level. Additionally, intranasal delivery of miR-125b decreased SPDEF protein levels, goblet cell differentiation, mucus hypersecretion, and altered relevant gene expressions. Taken together, these results suggest that miR-125b inhibits SPDEF expression modulating goblet cell differentiation and mucus secretion in asthma. © 2016 Elsevier B.V. All rights reserved.


Zhuang L.,Shanghai JiaoTong University | Bai M.,Xian Childrens Hospital | Zhou W.,Maternity and Child Care Hospital | Yu Y.,Shanghai JiaoTong University | And 3 more authors.
Journal of Medical Biochemistry | Year: 2014

Background: This study aimed to investigate the significance of MAMLD1 mutations in the incidence of hypospadias in a Chinese population. Methods: The experimental group consisted of 150 domestic children with hypospadias, aged 0.5 to six years and living in different provinces. A total of 120 normal children, aged two to six years, served as the control group. DNA was extracted for the direct sequencing of MAMLD1 genes. Results: Twelve cases (8.0%) of the missense mutation p.N589S were found in the experimental group, whereas four cases (3.0%) of the same mutation were found in the control group. No significant difference was observed in the mutation rate between the two groups (P>0.05). Four cases (2.7%) had a new missense mutation p.P567S in the experimental group, and three cases (2.5%) possessed the same mutation in the control group. No significant difference was observed between the two groups (P>0.05). Conclusions: In this study, the importance of repeated experiments in mutation-related studies was confirmed, which revealed the difference in predisposing genes among different populations. Although the mutation of the MAMLD1 gene had no apparent connection with the incidence of hypospadias in a Chinese population, a new mutation site of the MAMLD1 gene was discovered, which could provide new research topics for future studies.


Li L.,Taiyuan Maternity and Child Care Hospital | Meng Q.,Taiyuan Maternity and Child Care Hospital | Li S.,Maternity and Child Care Hospital | Kong Q.,Maternity and Child Care Hospital
Advanced Materials Research | Year: 2013

Although we have gained much information about lead-induced organ damage, the effect of blood lead level on T cell subgroup is yet to be determined. To assess the effect of blood lead level on T-lymphocyte Subpopulation Expression of children, and the association of T-lymphocyte Subpopulation Expression with threshold limit value of blood lead level.The aim of study is evaluating the significance of blood level, as an indicator for environmental lead, and T-lymphocyte Subpopulation Expression as an effect indicator, to determinate the correlation between the content of blood lead and T-lymphocyte Subpopulation Expression. The 120 children from area to exposure environmental lead were recruited into the study using immunofluorescence methods and study of blood lead level using graphite stove atom absorption spectrophotometer respectively, with blood lead level of 0.48μmol/L as a cut off value. The enrolled children according to their blood lead levels were assigned into three groups,26 in Group I with blood lead level ≥0.48 μmol/L, 40 in Group 2 with lead level ≥0.24 μmol/L but <0.48 μmol/L and 54 in Group 3 with lead level <0.24 μmol/L.Student t test was used in data analysis, and correlation analysis was used to assess the correlation between blood lead level and expression of T-lymphocyte Subpopulation.Data from all 120 children were used for data analysis. There was no significant difference when blood lead level of 0.24μmol/L as a cut off value. Our analysis of CD3, CD4 cells expression and CD4/CD8 cell ratio decreased in high lead groups of 0.48μmol/L as a cut off value(lead level ≥0.48 μmol/L) than Group (lead level ≥0.24 μmol/L) (t=3.27,P<0.01). When the blood lead level was ≥0.48 μmol/L, The result revealed the level of T-lymphocyte Subpopulation expression was a significant difference between groups of high and low blood lead level. There was difference correlation in the level of blood lead and T-lymphocyte Subpopulation expression. The result suggested that the high blood lead level may be regarded as an adverse effect on children's immune function especially on CD3 percentage, CD4/CD8 cell ratio when exposed environmental lead. © (2013) Trans Tech Publications, Switzerland.


PubMed | Maternity and Child Care Hospital and Shandong University
Type: | Journal: European journal of pharmacology | Year: 2016

Asthma is a disease characterized by goblet cell differentiation, mucus hypersecretion, airway inflammation, and airway hyperresponsiveness. miR-125b was downregulated as normal human bronchial epithelial cells differentiation to pseudostratified epithelium. However, its role in asthma remains unknown especially in regulating goblet cell differentiation. miR-125b expression in the sputum of 50 asthmatic children and 50 age- and sex-matched healthy controls were assessed by quantitative RT-PCR (qRT-PCR). Meanwhile, expressions of miR-125b and SAM pointed domain-containing ETS transcription factor (SPDEF) in normal human tracheal epithelial (HTEpC) and A549 cells stimulated with lipopolysaccharide (LPS) for 2h were detected by qRT-PCR and western blot. Furthermore, the predicted miR-125b target was determined in silico and confirmed with dual-luciferase reporter assay. Additionally, intranasal delivery of miR-125b mimic in mice was performed to study its effects on house dust mite-induced allergic airway inflammation mouse models. We found that miR-125b expression was decreased in the sputum of the asthmatic patients especially in eosinophilic asthma. After stimulation with LPS, miR-125b expression was downregulated, accompanied by the upregulation of SPDEF in HTEpC and A549 cells. Moreover, SPDEF is a target of miR-125b, which regulates SPDEF at the posttranscriptional level. Additionally, intranasal delivery of miR-125b decreased SPDEF protein levels, goblet cell differentiation, mucus hypersecretion, and altered relevant gene expressions. Taken together, these results suggest that miR-125b inhibits SPDEF expression modulating goblet cell differentiation and mucus secretion in asthma.


Zhang J.,Maternity and Child Care Hospital | Gao C.,Maternity and Child Care Hospital | Meng M.,Maternity and Child Care Hospital | Tang H.,Maternity and Child Care Hospital
Biomolecules and Therapeutics | Year: 2016

Acute myocardial infarction (AMI) remains a leading cause of morbidity and mortality worldwide. The exploration of new biomarkers with high sensitivity and specificity for early diagnosis of AMI therefore becomes one of the primary task. In the current study, we aim to detect whether there is any heart specific long noncoding RNA (lncRNA) releasing into the circulation during AMI, and explore its function in the neonatal rat cardiac myocytes injury induced by H2O2. Our results revealed that the cardiac-specific lncRNA MHRT (Myosin Heavy Chain Associated RNA Transcripts) was significantly elevated in the blood from AMI patients compared with the healthy control (*p<0.05). Using an in vitro neonatal rat cardiac myocytes injury model, we demonstrated that lncRNA MHRT was upregulated in the cardiac myocytes after treatment with hydrogen peroxide (H2O2) via real-time RT-PCR (qRT-PCR). Furthermore, we knockdowned the MHRT gene by siRNA to confirm its roles in the H2O2-induced cardiac cell apoptosis, and found that knockdown of MHRT led to significant more apoptotic cells than the non-target control (**p<0.01), indicating that the lncRNA MHRT is a protective factor for cardiomyocyte and the plasma concentration of MHRT may serve as a biomarker for myocardial infarction diagnosis in humans AMI. © 2016 The Korean Society of Applied Pharmacology.


Li S.,Maternity and Child Care Hospital | Fu K.,Maternity and Child Care Hospital | Kong Q.,Maternity and Child Care Hospital | Wu X.,Maternity and Child Care Hospital
Applied Mechanics and Materials | Year: 2012

Although we have gained much information about lead-induced organ damage, the effect of blood lead level on fresh blood quick native immune reaction on cancer cells is yet to be determined. The aim of study is evaluating the significance of blood level, as an indicator for environmental lead, and fresh blood quick native immune reaction on cancer cells as an effect indicator, to determinate the correlation between the content of blood lead and quick native immune reaction on cancer. The 120 children from area to exposure environmental lead were recruited into the study using cancer cells adding in fresh anti-coagulate blood to incubation at 37°C for 30 minutes and study of blood lead level using graphite stove atom absorption spectrophotometer, with blood lead level of 0.48μmol/L as a cut off value. blood lead level and fresh blood quick native immune reaction on cancer cells expression were measured with graphite furnace atomic absorption spectroscopy and using cancer cells adding in fresh anti-coagulate blood to incubation at 37°C for 30 minutes respectively. Student t test was used in data analysis, and correlation analysis was used to assess the correlation between blood lead level and expression of cancer cells adding in fresh anti-coagulate. Date from all 120 children was used for data analysis. Expression of TRR and TLR were lower in Group 1(lead level ≥0.48μmol/L) than Group 3(lead level 0.24μmol/L)(t=3.48, 2.32, P<0.01). When the blood lead level was ≥0.48μmol/L, the blood lead level showed significant inverse correlation with TRR and TLR(r=-0.703,-0.606 P<0.01). The result revealed the level of fresh blood quick native immune reaction on cancer cells was a significant difference between groups of high and low blood lead levels. There was correlation in the level of blood lead and fresh blood quick native immune reaction on cancer cells. The result suggested that the high blood lead level may be regarded as an adverse effect on children's immune function especially on TRR. TLR percentage when exposed environmental lead.

Loading Maternity and Child Care Hospital collaborators
Loading Maternity and Child Care Hospital collaborators