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Wu J.,Maternal and Child Health Hospital of Jinan City | Cai C.,Shanghai University | Tong D.,Shanghai JiaoTong University | Hou H.,Jian Guo
Genetic Testing and Molecular Biomarkers | Year: 2012

Despite the knowledge of many genetic alterations present in ovarian cancer, the complexity of this disease precludes placing its biology into a simple conceptual framework. Lysyl oxidase (LOX) is an extracellular matrix enzyme that catalyzes the cross-linking of collagens or elastin in the extracellular compartment. A novel polymorphism in the LOX gene, G473A (rs1800449), has been reported as being a risk factor for different diseases. The objective of this study was to investigate the association between the LOX G473A polymorphism and the susceptibility to ovarian cancer in the Chinese population. The LOX variant G473A was detected by a polymerase chain reaction-restriction fragment length polymorphism in 233 ovarian cancer cases and 246 age-matched controls. Data were analyzed using the chi-square test. Data showed that frequencies of the LOX 473AA genotype and the A allele were significantly higher in ovarian cancer patients than in controls (odds ratio [OR]=2.52, 95% confidence interval [CI] 1.28-4.96, p=0.006; and OR=1.62, 95% CI 1.18-2.20, p=0.002). In addition, the prevalence of the GA genotype, AA genotype, and A allele were significantly increased in recurrent ovarian cancer cases compared with primary ovarian cancer cases. Our data suggest that the G473A polymorphism of the LOX gene is associated with increased susceptibility to ovarian cancer. © 2012 Mary Ann Liebert, Inc.


Wang Y.,Fudan University | Xu W.,Fudan University | Zheng Z.,General Hospital of Shenyang Military Command | Wu J.,Maternal and Child Health Hospital of Jinan City | And 7 more authors.
Tumor Biology | Year: 2014

Invasive lobular carcinoma (ILC) of the breast is a rare type of breast cancer. Metastatic ILC is difficult to identify in sentinel lymph nodes (SLNs) because of its low-grade cytomorphology and its tendency to resemble lymphocytes. Touch imprint cytology (TIC) is a rapid method for evaluating SLNs intraoperatively. We sought to evaluate this technique in the identification of SLN metastases in ILC. A total of 230 SLNs from 88 patients diagnosed with ILC were examined with TIC, and the results were compared with their histological diagnosis. We first confirmed some typical cytological features of SLN metastases in ILC such as a thick eosinophilic background and hyperplasia of small cell lacking adhesion. Further analysis showed that sensitivity of TIC was 60.5 % on per-node basis and 58.3 % on per-patient basis; specificity of TIC was 100 % on per-node basis and 100 % on per-patient basis. Interestingly, when we divided patients according to their ages, the sensitivity of TIC in patients younger than 50 years old greatly increased to 90.9 % on per-node basis and 85.7 % on per-patient basis, whereas the specificity remained 100 % on both per-node basis and per-patient basis. However, different tumor sizes did not clearly change the sensitivity of TIC compared to the overall sensitivity. Patients with tumor size bigger than 1 cm revealed a TIC sensitivity of 59.4 % on per-node basis and 55.0 % on per-patient basis. These results suggest that TIC can be used as a reliable method of detecting SLN metastasis only in young patients with ILC. © 2014 International Society of Oncology and BioMarkers (ISOBM).


Bu M.,Maternal and Child Health Hospital of Jinan City | Li L.,Xinjiang Medical University | Zhang Y.,Maternal and Child Health Hospital of Jinan City | Xu Y.,Maternal and Child Health Hospital of Jinan City | And 3 more authors.
DNA and Cell Biology | Year: 2014

Lysyl oxidase (LOX) is a copper-dependent amine oxidase that plays important roles in the homeostasis of tumors. The aim of this study was to investigate the association between LOX polymorphisms and cervical cancer, and the effect of these polymorphisms on gene expression. We evaluated two polymorphisms of LOX, rs1800449G/A (G473A) and rs2278226C/G, in 262 cervical cancer cases and 298 healthy controls in the Chinese population. Results showed that the prevalence of rs1800449AA genotype was significantly increased in cases than in controls (p=0.004). Individuals who carried the rs1800449A allele had a 1.56-fold increased risk for cervical cancer than those with the rs1800449G allele (p=0.003). The rs2278226CG genotype also revealed a significantly higher proportion in cases (20.6%) than in controls (7.7%, p<0.001). Interestingly, when analyzing these two polymorphisms with the serum level of LOX, we identified that cervical cancer patients carrying the rs2278226CG genotype had a significantly elevated level of LOX than those with rs2278226CC wild type, whereas the same phenomenon was not observed in controls. The rs1800449 polymorphism did not affect the LOX serum level in either controls or patients. These results suggest that the polymorphisms in the LOX gene may be involved in the development of cervical cancer through various mechanisms. © Copyright 2014, Mary Ann Liebert, Inc. 2014.

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