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Cai J.,Wenzhou University | Ma J.,Wenzhou University | Xu K.,Central South University | Gao G.,Central South University | And 2 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015

Radix Sophorae Tonkinensis (S. tonkinensis) is the processed lateral root of Sophora subprostrata (Leguminosae) that widely distributed over the southwest China. Radix Sophorae Tonkinensis has been widely used as a Chinese medicinal herb for the treatment of disease such as jaundice, inflammation, and aches. Herein, in order to investigate the effects of Radix Sophorae Tonkinensis on the metabolic capacity of rat cytochrome P450 (CYP) enzymes, we employed a cocktail method to evaluate the activities of CYP1A2, CYP2D6, CYP3A4, CYP2C19, CYP2C9 and CYP2B6. The experimental rats were randomly divided into two groups (control group and Radix Sophorae Tonkinensis treated group). The Radix Sophorae Tonkinensis treated group rats were given 5 g/kg Radix Sophorae Tonkinensis by continuous intragastric administration for 14 days. The mixture of six probes (phenacetin, metroprolol, midazolam, omeprazole, tolbutamide and bupropion) was given to rats by intragastric administration. The concentrations of probe drugs in rat plasma were measured by UPLC-MS/MS. The results showed that continu­ous intragastric administration for 14 days may inhibit the activities of rat CYP450 isoforms CYP2D6, CYP2C19 and CYP2B6. This finding may provide guidance for rational clinical uses of Radix Sophorae Tonkinensis. © 2015, E-Century Publishing Corporation. All rights reserved.

Ouyang N.,Central South University | Luo J.,Central South University | Du Q.,Maternal and Child Health Care Hospital of Hunan Province | Liu Z.,Maternal and Child Health Care Hospital of Hunan Province
Journal of Central South University (Medical Sciences) | Year: 2011

Objective To explore the relation between environmental factors and the occurrence of congenital heart disease (CHD). Methods A hospital-based case-control study was conducted. Mothers of 123 patients with congenital heart disease and 246 normal newborns were interviewed with standardized questionnaires. Chi-square test and logistic regression models were performed to analyze the influencing factors. Results As shown in multivariable logistic model, gravida with occupational exposure(0/? =4. 10) ,or gravida with chronic diseases during progestational pregnancy (OR =5.95) , gravida with abnormal childbearing history (OR =6.27) , and gravida catching a cold in the early stage of pregnancy (OR =2.07) would increase the risk of CHD. On the contrary, eating meat, egg (OR=0. 18)and milk (OR =0.23) , and taking multivitamin and microelement (OR =0. 35)during the pregnancy reduced the risk of CHD. Conclusion The risk of the offspring developing CHD is associated with gravida' s exposure to many environmental factors during pregnancy. It is time to strengthen the intervention measures to reduce the occurrence of CHD.

Xiong X.,Maternal and Child Health Care Hospital of Hunan Province | Liu D.,Maternal and Child Health Care Hospital of Hunan Province | Wang Y.,Maternal and Child Health Care Hospital of Hunan Province | Zeng T.,Maternal and Child Health Care Hospital of Hunan Province | Peng Y.,Maternal and Child Health Care Hospital of Hunan Province
BioMed Research International | Year: 2016

Autism spectrum disorders (ASDs) are a group of mental illnesses highly correlated with gut microbiota. Recent studies have shown that some abnormal aromatic metabolites in autism patients are presumably derived from overgrown Clostridium species in gut, which may be used for diagnostic purposes. In this paper, a GC/MS based metabolomic approach was utilized to seek similar biomarkers by analyzing the urinary information in 62 ASDs patients compared with 62 non-ASDs controls in China, aged 1.5-7. Three compounds identified as 3-(3-hydroxyphenyl)-3-hydroxypropionic acid (HPHPA), 3-hydroxyphenylacetic acid (3HPA), and 3-hydroxyhippuric acid (3HHA) were found in higher concentrations in autistic children than in the controls (p < 0.001). After oral vancomycin treatment, urinary excretion of HPHPA (p < 0.001), 3HPA (p < 0.005), and 3HHA (p < 0.001) decreased markedly, which indicated that these compounds may also be from gut Clostridium species. The sensitivity and specificity of HPHPA, 3HPA, and 3HHA were evaluated by receiver-operating characteristic (ROC) analysis. The specificity of each compound for ASDs was very high (>96%). After two-regression analysis, the optimal area under the curve (AUC, 0.962), sensitivity (90.3%), and specificity (98.4%) were obtained by ROC curve of Prediction probability based on the three metabolites. These findings demonstrate that the measurements of the three compounds are strong predictors of ASDs and support the potential clinical utility for identifying a subgroup of ASDs subjects. © 2016 Xiyue Xiong et al.

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