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Chen L.-Z.,Central South University | Zhou W.-Q.,Central South University | Zhao S.-S.,Central South University | Liu Z.-Y.,Maternal and Child Health Hospital of Hunan Province | And 3 more authors.
World Journal of Gastroenterology | Year: 2011

AIM: To examine the determinants of maternal-neonatal transmission of hepatitis B virus (HBV). METHODS: A nested case-control study was conducted in Changsha, Hunan, People's Republic of China from January 1, 2005 to September 31, 2006.To avoid potential maternal blood contamination, we collected vein blood of newborns immediately after birth and before initial hepatitis B vaccination to determine the HBV infection status of the newborn.For each HBsAg-positive infant, one HBsAg-negative infant born to an HBsAg-positive mother was matched by hospital at birth (same), gender (same), and date of birth (within 1 mo).A face-to-face interview was conducted to collect clinical and epidemiological data.Conditional logistic regression analysis was used to estimate the independent effects of various determinants on maternal-neonatal transmission of HBV. RESULTS: A total of 141 HBsAg-positive infants and 141 individually matched HBsAg-negative infants were included in the final analysis.Maternal first-degree family history of HBV infection, intrahepatic cholestasis, and premature rupture of membranes were risk factors for perinatal transmission of HBV, whereas systematic treatment and HBV immunoglobulin injections for mothers with HBV infection were protective factors for maternal-neonatal transmission of HBV, after adjustment for potential confounding factors. CONCLUSION: For HBsAg-positive mothers, systematic treatment, HBV immunoglobulin administration, and controlling intrahepatic cholestasis and pregnancy complications may reduce the incidence of perinatal transmission of HBV. ©2011 Baishideng. All rights reserved.


Zhu L.,Hunan Normal University | Xu L.,Hunan Normal University | Tan L.,Hunan Normal University | Tan H.,Maternal and Child Health Hospital of Hunan Province | And 2 more authors.
Talanta | Year: 2013

An electrochemical cholesterol biosensor based on the direct electron transfer of cholesterol oxidase (ChOx) immobilized on gold nanoparticles- decorated multiwalled carbon nanotubes (GNPs-MWCNTs) was fabricated. GNPs-MWCNTs were prepared based on the reduction of HAuCl4 in the presence of carboxyl group functionalized MWCNTs. Transmission electron microscopy image shows that rounded gold nanoparticles with diameters of 6-10 nm were decorated on carbon nanotube surfaces. ChOx was directly adsorbed on the nanocomposite modified glassy carbon electrode and protected by a Nafion film. Direct electrochemistry of ChOx on the electrode surface was obtained, proved by one pair of well-defined, quasi-reversible redox peaks in phosphate buffered saline. Under optimized conditions, the fabricated electrode displayed a linear response in the cholesterol concentration range from 0.0100 to 5.00 mmol L -1 with a detection limit of 4.3 μmol L-1 estimated at a signal-to-noise ratio of 3. The apparent Michaelis-Menten constant was measured to be 0.29 mmol L-1, indicating that the immobilized ChOx on GNPs-MWCNTs matrix retained its native activity. The developed biosensor presented good selectivity, repeatability, reproducibility and stability. The concentration of free cholesterol in a human serum sample, detected by using the developed biosensor, is in good agreement with that determined by the well-established spectrophotometric method. © 2012 Elsevier B.V.


Tang A.-Q.,Maternal and Child Health Hospital of Hunan Province | Cao X.-C.,Hunan Normal University | Tian L.,Hunan Normal University | He L.,Hunan Normal University | Liu F.,Hunan Normal University
Molecular Medicine Reports | Year: 2015

Casein kinase 2 (CK2) is a protein kinase which is frequently activated in cancer. The Hedgehog (Hh) signaling pathway is involved in the stimulation of cancer stem cell growth. Its aberrant activation has been validated in several types of cancer, including ovarian cancer. In the present study, the sphere-forming cells (SFCs) of the human ovarian cancer SKOV3 cell line were observed to have self-renewal capacity, indicating the possession of ovarian cancer stem-like cell properties. SKOV3-derived SFCs had higher levels of CK2α and glioma-associated oncogene 1 (Gli1) proteins compared with those of parental cells. Apigenin, a common flavonoid, significantly inhibited the self-renewal capacity and the protein expression of CK2α and Gli1 proteins in the SKOV3-derived SFCs, which occurred in a concentration-dependent manner. In addition, CK2α small interfering RNA downregulated the protein expression of CK2α and Gli1 and synergistically inhibited the self-renewal capacity of the SKOV3-derived SFCs with apigenin. However, forced overexpression of CK2α resulted in an increase in the expression of CK2α and Gli1 and attenuated the apigenin-inhibited self-renewal effect in the SKOV3-derived SFCs. These results suggested that apigenin inhibited the self-renewal capacity of SKOV3-derived SFCs and was involved in downregulating the expression of Gli1 by the inhibition of CK2α.


Chen Y.,Maternal and Child Health Hospital of Hunan Province | Shi X.,Central South University
Journal of Obstetrics and Gynaecology Research | Year: 2016

Aim: To investigate the effects of repeated mifepristone and levonorgestrel use on estrous cycle and expression of ovarian follicle-stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) in mice. Methods: Ovarian FSHR and LHR mRNA expression was measured using real-time quantitative reverse transcription–polymerase chain reaction, while the protein levels were measured using immunohistochemistry. Results: Repeated use of mifepristone and levonorgestrel significantly lengthened the estrous cycle and decreased FSHR and LHR mRNA and protein expression in the ovaries of mice at 4, 24, and 48 days after discontinuing drug use. Repeated use of mifepristone and levonorgestrel had significant main effects on estrous cycle length and the mRNA expression and protein level of ovarian FSHR and LHR. Repeated mifepristone and levonorgestrel use and withdrawal time had a significant interaction with mouse estrous cycle (F = 16.65, P < 0.05), ovarian LHR and FSHR mRNA expression (F = 563.072, P < 0.05), and protein level (F = 6.536, P < 0.05). Conclusion: Repeated use of mifepristone and levonorgestrel can lead to sustained damage to ovarian function through inhibition of ovarian FSHR and LHR expression in mice. © 2016 Japan Society of Obstetrics and Gynecology


Qin J.,Maternal and Child Health Hospital of Hunan Province | Qin J.,Central South University | Wang H.,Maternal and Child Health Hospital of Hunan Province | Sheng X.,Maternal and Child Health Hospital of Hunan Province | And 3 more authors.
Fertility and Sterility | Year: 2015

Objective To provide an up-to-date comparison of pregnancy-related complications and adverse pregnancy outcomes of multiple pregnancies generated with assisted reproductive technology (ART) vs. spontaneous conception. Design Meta-analysis. Setting University-affiliated teaching hospital. Patient(s) Multiple pregnancies conceived by ART or naturally. Intervention(s) Searches through October 2014 were conducted on PubMed, Google Scholar, Cochrane Libraries, China Biology Medicine disc, Chinese Scientific Journals Fulltext Database, China National Knowledge Infrastructure, and Wanfang Data, to identify studies that met prestated inclusion criteria. Either a fixed- or a random-effects model was used to calculate the overall combined risk estimates. Subgroup analysis was performed to explore potential heterogeneity moderators. Main Outcome Measure(s) Pregnancy-related complications and adverse pregnancy outcomes. Result(s) Thirty-nine cohort studies involving 146,008 multiple births were included in the meta-analysis. Multiple pregnancies from ART were associated with a higher risk of premature rupture of membranes (relative risk [RR] = 1.20, 95% confidence interval [CI]: 1.05-1.37; I2 = 15%); pregnancy-induced hypertension (RR = 1.11, 95% CI: 1.04-1.19; I2 = 6%); gestational diabetes mellitus (RR = 1.78, 95% CI: 1.25-2.55; I2 = 42%); preterm birth (RR = 1.08, 95% CI: 1.03-1.14; I2 = 83%); very preterm birth (RR = 1.18, 95% CI: 1.04-1.34; I2 = 79%); low birth weight (RR = 1.04, 95% CI: 1.01-1.07; I2 = 47%); very low birth weight (RR = 1.13, 95% CI: 1.01-1.25; I2 = 62%); and congenital malformation (RR = 1.11, 95% CI: 1.02-1.22; I2 = 30%). The relevant heterogeneity moderators have been identified by subgroup analysis. Sensitivity analysis yielded similar results. No evidence of publication bias was observed. Conclusion(s) Although the role of potential bias and evidence of heterogeneity should be carefully evaluated, the present study suggests that multiple pregnancies generated via ART, vs. spontaneous conception, are associated with higher risks of pregnancy-related complications and adverse pregnancy outcomes. Further research is needed to determine which aspect of ART poses the most risk and how this risk can be minimized. © 2015 American Society for Reproductive Medicine.


Liu Z.Y.,Maternal and Child Health Hospital of Hunan Province
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi | Year: 2012

To understand the situation of depression and its related influencing factors among medical staff in Hunan province. Data were collected through random sampling with multi-stage stratified cluster. Wilcoxon rank sum test, Kruskal-Wallis H test and Ordinal regression analysis were used for data analysis by SPSS 17.0 software. This survey was including 16,000 medical personnel with 14, 988 valid questionnaires and the effective rate was 93.68%. from the single factor analysis showed that factors as: level of the hospital grading, gender, education background, age, occupation, title, departments, the number of continue education, income, working overtime every week, the frequency of night work, the number of patients treated in the emergency room etc., had statistical significances (P < 0.05). Data from ordinal regression showed that the probabilities related to depression that clinicians and nurses suffering from were 1.58 times more than the pharmacists (OR = 1.58, 95%CI: 1.30 - 1.92). The probability among those whose income was less than 2000 Yuan/month was 2.19 times of the ones whose earned more than 3000 Yuan/month (OR = 2.19, 95%CI: 2.05 - 2.35). The higher the numbers of days with working overtime every week, the frequencies of night work, and the numbers of patients being treated at the emergency room, with more probabilities of the people with depression seen in our study. Depression seemed to be common among doctors and nurses. We suggested that the government need to increase the monthly income and to reduce the workload and intensity, lessen the overworking time, etc.


Bai M.,Central South University | Zhang L.,Central South University | Zhang L.,Maternal and Child health Hospital of Hunan Province | Zhu X.,Central South University | And 3 more authors.
Physiology and Behavior | Year: 2014

Depression is a severe psychiatric disorder, which is a huge burden on both the individual and society as a whole. Neurobiological studies of depression on humans are limited by ethics. Animal models established by stressor stimulation are currently effective tools for the neurobiological study of depression. In this study, we comparatively analyzed behavioral heterogeneity, gender, and individual differences in animal models reflecting early life stress, adverse events in adulthood, or combined early life stress and stressful events in adulthood. Results demonstrated that these three different stressors induced dissimilar depressive behaviors. Maternal deprivation (MD) induced severe anhedonia. Chronic unpredictable stress (CUPS) induced the most severe decrease in desperation behavior, moderate anhedonia, and moderate loss of interest in exploration of the surroundings. Dural stress (DS) exposure caused the most severe decline in interest in exploring the surroundings. Male rats all exhibited some form of depressive behavior after they were exposed to MD, CUPS and DS. In contrast, no depressive performance was observed in female rats after they were exposed to MD, and the CUPS only decreased the total distance the rats crawled in the open field test. Rats exhibited more obvious individual differences in floating time than in the vertical activity, total distance and sucrose preference rate when experiencing stress. Our study suggests that different stressors may induce different depression subtypes and that the observed differences in the prevalence of depression between genders in the clinic may be due to effect of psychosocial factors which affects humans more strongly than rats. Our study also suggests that individual difference is more obvious in desperation behaviors than that in exploratory interest and anhedonia when the individual experienced stress. © 2014 Elsevier Inc.


Qin J.,Maternal and Child Health Hospital of Hunan Province | Qin J.,Central South University | Liu X.,Central South University | Sheng X.,Maternal and Child Health Hospital of Hunan Province | And 2 more authors.
Fertility and Sterility | Year: 2016

Objective To determine whether there are any increases in pregnancy-related complications and adverse pregnancy outcomes in singleton pregnancies after assisted reproductive technology (ART) compared with those conceived naturally. Design Meta-analysis. Setting University-affiliated teaching hospital. Patient(s) Singleton pregnancies conceived with ART and naturally. Intervention(s) PubMed, Google Scholar, Cochrane Libraries and Chinese database were searched through March 2015 to identify studies that met pre-stated inclusion criteria. Either a fixed- or a random-effects model was used to calculate the overall combined risk estimates. Subgroup analysis was performed to explore potential heterogeneity moderators. Main Outcome Measure(s) Pregnancy-related complications and adverse pregnancy outcomes. Result(s) Fifty cohort studies comprising 161,370 ART and 2,280,241 spontaneously conceived singleton pregnancies were identified. The ART singleton pregnancies had a significantly increased risk of pregnancy-induced hypertension (relative risk [RR] 1.30, 95% confidence interval [CI] 1.04-1.62; I2 = 79%), gestational diabetes mellitus (RR 1.31, 95% CI 1.13-1.53; I2 = 6%), placenta previa (RR 3.71, 95% CI 2.67-5.16; I2 = 72%), placental abruption (RR 1.83, 95% CI 1.49-2.24; I2 = 22%), antepartum hemorrhage (RR 2.11, 95% CI 1.86-2.38; I2 = 47%), postpartum hemorrhage (RR 1.29, 95% CI 1.06-1.57; I2 = 65%), polyhydramnios (RR 1.74, 95% CI 1.24-2.45; I2 = 0%), oligohydramnios (RR 2.14, 95% CI 1.53-3.01; I2 = 0%), cesarean sections (RR 1.58, 95% CI 1.48-1.70; I2 = 92%), preterm birth (RR 1.71, 95% CI 1.59-1.83; I2=80%), very preterm birth (RR 2.12, 95% CI 1.73-2.59; I2 = 90%), low birth weight (RR 1.61, 95% CI 1.49-1.75; I2 = 80%), very low birth weight (RR 2.12, 95% CI 1.84-2.43; I2 = 67%), small for gestational age (RR 1.35, 95% CI 1.20-1.52; I2 = 82%), perinatal mortality (RR 1.64, 95% CI 1.41-1.90; I2=45%), and congenital malformation (RR 1.37, 95% CI 1.29-1.45; I2=41%). Relevant heterogeneity moderators have been identified by subgroup analysis. Sensitivity analysis yielded consistent results. No evidence of publication bias was observed. Conclusion(s) The ART singleton pregnancies are associated with higher risks of adverse obstetric outcomes. Obstetricians should manage these pregnancies as high risk. © 2016 American Society for Reproductive Medicine.


Ding H.,Central South University | Wu Y.-L.,Central South University | Wang Y.-X.,Maternal and Child Health Hospital of Hunan Province | Zhu F.-F.,Central South University
Asian Pacific Journal of Cancer Prevention | Year: 2014

Objectives: MicroRNAs (miRNAs) are important regulators of many physiological and pathological processes, including tumorigenesis and metastasis. In this study, we sought to determine the underlying molecular mechanisms of metastatic cervical carcinoma by performing miRNA profiling. Methods: Tissue samples were collected from ten cervical squamous cancer patients who underwent hysterectomy and pelvic lymph node (PLN) dissection in our hospital, including four PLN-positive (metastatic) cases and six PLN-negative (non-metastatic) cases. A miRNA microarray platform with 1223 probes was used to determine the miRNA expression profiles of these two tissue types and case groups. MiRNAs having at least 4-fold differential expression between PLN-positive and PLN-negative cervical cancer tissues were bioinformatically analyzed for target gene prediction. MiRNAs with tumor-associated target genes were validated by quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results: Thirty-nine miRNAs were differentially expressed (>4-fold) between the PLN-positive and PLN-negative groups, of which, 22 were up-regulated and 17 were down-regulated. Sixty-nine percent of the miRNAs (27/39) had tumor-associated target genes, and the expression levels of six of those (miR-126, miR-96, miR-144, miR-657, miR-490-5p, and miR-323-3p) were confirmed by quantitative (q)RT-PCR. Conclusions: Six MiRNAs with predicted tumor-associated target genes encoding proteins that are known to be involved in cell adhesion, cytoskeletal remodeling, cell proliferation, cell migration, and apoptosis were identified. These findings suggest that a panel of miRNAs may regulate multiple and various steps of the metastasis cascade by targeting metastasis-associated genes. Since these six miRNAs are predicted to target tumor-associated genes, it is likely that they contribute to the metastatic potential of cervical cancer and may aid in prognosis or molecular therapy.


PubMed | Maternal and Child Health Hospital of Hunan Province
Type: Journal Article | Journal: Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics | Year: 2016

To investigate the phenotype-genotype association of isodicentromere Y chromosome by analysis of two female patients carrying the chromosome with sexual development disorders.The karyotypes of the two patients were determined by application of conventional G banding of peripheral blood samples and fluorescence in situ hybridization (FISH). PCR was applied to detect the presence of SRY gene.Conventional karyotype analysis showed case 1 to be a mosaic: mos.45,X[38]/46,X,+mar[151]/47,XY,+mar[5]/47,X,+mar 2[2]/46,XY[4], FISH showed that 12 different cell lines were presented in the karyotype of case 1 and partial cell lines with SRY gene, the marker is an isodicentromere Y chromosome [idic(Y)(p)]. No mutation was found in the SRY gene. The karyotype of case 2 was mos.45,X[25]/46,X,+mar[35]. FISH showed the marker to be an idic(Y)(p) without the SRY gene.The karyotype of patients carrying idic(Y)(p) seems unstable, and female patients have the characteristics of short stature and secondary sexual hypoplasia. Karyotype analysis combined with FISH analysis can accurately determine the breakpoint of idic(Y) and identify the types of complex mosaic, which may facilitate genetic counseling and prognosis.

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