Deng S.,Hubei University of Medicine |
Yu X.,Hubei University of Medicine |
Ke X.,Hubei University of Medicine |
Qu P.,Hubei University of Medicine |
And 3 more authors.
Medical Journal of Wuhan University | Year: 2012
Objective: To investigate the effect of Rapamycin (RA) on the proliferation apoptosis and drug sensitivity of human endometrial carcinoma line Ishikawa cells in vitro. Methods: Cell survival rate and cell apoptosis rate of Ishikawa cells were evaluated by clone formation test and flow cytometry after treatment of 10 μmol/L RA alone or combined respectively with 50% of half maximal inhibitory concentration (IC 50) of the ADR, DDP, and PTX for 24 hours. The mRNA levels of mTOR after treatment was detected by RT-PCR. Western blot was used to measure Bcl-2 protein abundance after treatment with 10 μmol/L RA for 6, 12, and 24 hours respectively. Results: Rapamycin significantly decreased the mRNA expressions of mTOR and the abundance of Bcl-2 protein. Compared with the treatment with RA alone, combined treatment significantly increased cell apoptosis rate, decreased cell survival rate and improved the cytotoxicity of the chemotherapeutic agents (P<0.05). Conclusion: Rapamycin can block the mTOR pathway and decrease Bcl-2 protein to inhibit the proliferation, induce the apoptosis and enhance the chemosensitivity of Ishikawa cells.