Lu J.,Maternal and Child Health Hospital |
Ting Y.H.,Chinese University of Hong Kong |
Law K.M.,Chinese University of Hong Kong |
Lau T.K.,Fetal Medicine Center |
Leung T.Y.,Chinese University of Hong Kong
Fetal Diagnosis and Therapy | Year: 2013
Objective: To evaluate the perinatal outcome of monochorionic (MC) multiple pregnancies after selective reduction by radiofrequency ablation (RFA). Methods: A case series of all MC multiple pregnancies with selective reduction by RFA in one single institution was reviewed. Results: Ten consecutive patients with an MC pregnancy (9 pairs of twins and 1 set of triplets) underwent RFA. The median gestational age at the time of the procedure was 15.6 weeks (range, 12.3-19.6). The indications for selective reduction included discordance for fetal anomalies (4 cases), twin reversed arterial perfusion sequence (3 cases), selective intrauterine growth restriction (2 cases) and severe twin-twin transfusion syndrome (1 case). All procedures were technically successful in achieving selective reduction. The overall survival rate of the co-twin was 81.8% (9/11), and the median gestational age at delivery was 35.9 weeks (range, 32.4-38.6). There was one preterm delivery before 34 weeks of gestation (11.1%). Preterm premature rupture of the membranes occurred in 2 patients (20%); however, this was not observed within 4 weeks postoperatively, nor did they deliver before 32 weeks. Conclusions: RFA is a promising technique for selective reduction in complicated MC multiple pregnancies with a high survival rate and low complication rate. © 2013 S. Karger AG, Basel.
Qin Y.,Shandong University of Technology |
Zhao H.,Shandong University of Technology |
Xu J.,Fudan University |
Shi Y.,Shanghai JiaoTong University |
And 11 more authors.
Human Molecular Genetics | Year: 2012
Premature ovarian failure (POF) is a complex heritable disorder known to be caused by chromosomal abnormalities and to date a limited number of known mutations, often autosomal. We sought to identify additional genetic loci associated with POF by performing the first large-scale genome-wide association study (GWAS). GWAS, using Affymetrix SNP 6.0 chip, was conducted in an initial discovery set of 391 well-documented (follicle-stimulating hormone >40 IU/ml) Chinese Han POF patients, compared with 895 unrelated Chinese female controls. A replication study on the most significant loci was then performed in an independent set of 400 cases and 800 controls. Suggestive significant associations were observed at 8q22.3. Replication of eight single-nucleotide polymorphisms (SNPs) (rs10464815, rs10808365, rs3847152, rs3847153, rs3847154, rs3843552, rs10955242, rs3843555) (P ≤ 3.86 × 10 -6) was confirmed in verification sets. No specific candidate gene was found in the immediate region of 8q22.3. This GWAS, involving by far the largest sample of POF cases accumulated to date, revealed heretofore unrecognized association between POF and a novel genetic locus or region of unknown nature on 8q22.3. We speculate existence of a long-distance regulatory region that has relevance to the control of ovarian differentiation or oogenesis. Given failure to find association with any of the other autosomal regions known to harbor genes causing ovarian failure, our findings also underscore the likelihood of considerable genetic and etiologic heterogeneity in POF and the need for additional approaches like whole-genome sequencing. © The Author 2011. Published by Oxford University Press. All rights reserved.
Xie D.,Maternal and Child Health Hospital |
Nie G.,Peking University
Traffic Injury Prevention | Year: 2012
Objective: China has been concerned about the serious problem of drinking and driving road crashes, and it has made good progress by establishing strict laws, imposing serious penalties, and initiating a rigorous enforcement program since 2008. This study has assessed the magnitude and nature of the problem and reviewed the legislation, current practices, and institutional capacities for preventing drinking and driving.Methods: Data and information were collected using existing reports and by consulting officials and experts from a number of agencies.Results: Although there were no national statistics on levels of drinking and driving, random breath test surveys in 2 southern cities showed that between 4.5 and 4.6 percent of drivers were driving over the minimum legal blood alcohol concentration (BAC) limit of 20 mg/100 mL. Preliminary results from crash data also showed that at least 20 percent of serious road crashes were alcohol related in these cities. The national published figure for fatal crashes caused by drinking and driving was much lower, only 4 percent, but alcohol was not often identified as the main cause because of measurement difficulties. China's legislation sets 2 BAC limits that are comparable with international norms. It has recently increased the penalties for drunk driving, the more serious of the 2 offenses, with a minimum driving ban of 5 years. The police are actively enforcing the laws through frequent roadside checking but they need more resources. Alcohol breath tests before and after a combined publicity and enforcement campaign indicated reductions of 87 and 68 percent of drivers over the legal limit in 2 southern cities.Conclusions: China has made progress in strengthening its approach to preventing drinking and driving, particularly in the area of law enforcement. However, it is not possible to evaluate the potential benefits because of data issues. Recommendations for the future include the need to improve the national road crash and injury database, strengthen the coordination of key agencies, and provide more effective and sustained public information campaigns that target vulnerable drivers and are integrated with enforcement strategies. Evaluation and research are important to improve future prevention programs. © 2012 Taylor and Francis Group, LLC.
Guo Q.,Maternal and Child Health Hospital |
Xiao L.,Maternal and Child Health Hospital |
Zhou Y.,Maternal and Child Health Hospital
Clinical Chemistry | Year: 2012
BACKGROUND: Several molecular methods, such as quantitative fluorescence PCR and multiplex ligation-dependent probe amplification, currently serve as important adjuncts to traditional karyotyping for the diagnosis of aneuploidy; however, the performance or throughput limitations of these methods hinder their use for routine prenatal diagnosis and population-based postnatal screening. We developed a novel approach, called "high-resolution melting analysis of segmental duplications," to detect common aneuploidies. METHODS: In this method, similar sequences located on different chromosomes are amplified simultaneously with a single primer set; the PCR products are then analyzed by high-resolution melting. Aneuploidy-associated dosage abnormalities produce different ratios of similar amplicons, which produce melting curves that are detectably different from those of samples from unaffected individuals. We applied this method to DNA samples isolated from individuals with trisomy 21 (n=48), trisomy 18 (n=10), trisomy 13 (n = 3), 45,X (n = 8), and 47,XXY (n = 14), and from unaffected controls (n = 48). RESULTS: As judged by the karyotyping results, our method attained 100% diagnostic sensitivity and 99.6% diagnostic specificity. Moreover, our method was able to detect a change in chromosome dosage as low as 1.05-fold. CONCLUSIONS: This novel method clearly differentiates samples of patients with common aneuploidies from those of unaffected controls, while markedly simplifying the assays and reducing time and costs. The assay has sufficient throughput to meet the demands of large-scale testing, such as population-based postnatal screening, and is thus suitable for routine use. © 2012 American Association for Clinical Chemistry.
Zhang Z.,Maternal and Child Health Hospital
Clinical and Experimental Obstetrics and Gynecology | Year: 2016
Ruptured vasa previa in term pregnancy is rare but usually catastrophic if emergency delivery is not achieved. The authors present a case of ruptured vasa previa in velamentous cord insertion placenta. The fetus survived after intensive treatment immediately after delivery by cesarean section, but, unfortunately, died after the family gave him up. Defects in the vessel wall architecture were visualized and confirmed by histopathologic examination and might be responsible for the vessel rupture. Prenatal sonographic identification of cord insertion site into the placenta is encouraged as standard of practice to prevent this accident.
Fan Y.F.,Maternal and Child Health Hospital
Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] | Year: 2010
To study the relationship of bodyweight gain and the occurrence of gestational diabetes mellitus (GDM) during pregnancy, and analyze the effect of the nutritional therapy on the outcome of GDM. We collected 265 pregnant women who were diagnosed to be GDM and 571 pregnant women as the control group in the Xiamen Maternal and Child Health Hospital during 2007 - 2009. The general information of the subjects were collected. The bodyweight of the subjects were measured before the 20(th) week of pregnancy, 26 - 27(th) week (mid-gestation), 35 - 36(th) week (late-gestation) of pregnancy and prior to delivery. The bodyweight gain of different pregnancy weeks of the two groups and the effect of bodyweight on GMD occurrence before 28(th) week of pregnancy were analyzed by ages (< 25, 25-, 30-, ≥ 35). Meanwhile, we prescribed the nutrition therapy to the GDM pregnant woman and the effect of the blood sugar control on the outcome of the pregnancy were evaluated. The bodyweight gain of 25-, 30-, older than 35 year-old subjects of the GDM group were (16.9 ± 6.3), (16.8 ± 6.1), (16.5 ± 6.0) kg, respectively, the bodyweight gain of the control group were (13.9 ± 3.0), (13.8 ± 2.7), (13.3 ± 2.7) kg (t = 6.259, 5.885, 3.533, respectively, all P values < 0.05). During the 20(th) to 27(th) week of the pregnancy, the bodyweight gain of the subjects younger than 25, 25-, 30-year-old in GDM group were (5.2 ± 1.0), (5.4 ± 1.7), (4.8 ± 1.3) kg, respectively, the bodyweight gain of the control group were (3.3 ± 1.3), (3.7 ± 1.6) and (3.5 ± 0.7) kg (t = 5.026, 9.659, 11.19, respectively, all P values < 0.05). During the period between 26(th) to 36(th) week, the bodyweight gain of subjects older than 35 year-old in GDP group was (3.6 ± 2.0) kg which was less than the control group ((4.0 ± 0.9) kg, t = -2.449, P < 0.05). 41.22% (54/131) and 44.94% (40/89) of 25-, 30-year-old subjects in GDM group showed bodyweight gain more than 13 kg, but 30.04% (76/253) and 26.07% (55/211) in the control group (OR values were 1.633 and 2.315, both P values < 0.05). The rate of the abnormal birth weight of the GDM group with blood sugar controlled and the control group were 6.6% (12/182) and 9.4% (54/571) which was lower than the GDP group with blood sugar control failure (20.5% (17/83)) (χ(2) values were 11.460, 9.119, respectively, both P values < 0.0125). The rate of premature delivery was 21.7%(18/83), higher than the control group (10.8%, 62/571) (χ(2) = 7.945, P < 0.0125). The rate of the cesarean in the control group was 25.4%(145/571) which was lower than the two GDM groups, including the group which the blood sugar was well controlled (46.7%, 85/182) and not well controlled (65.0%, 54/83) (χ(2) values were 29.540, 53.860, respectively, both P values < 0.0125). The bodyweight gain in the mid-gestation could affect the occurrence of GDM. The bodyweight gain should be less than 13 kg before 28(th) week of the pregnancy whose age was 25-year-old. Nutritional therapy and blood sugar control in GDM pregnant women could improve the pregnancy outcome.
Zhu P.,Anhui Medical University |
Tao F.,Anhui Medical University |
Hao J.,Anhui Medical University |
Sun Y.,Anhui Medical University |
And 2 more authors.
American Journal of Obstetrics and Gynecology | Year: 2010
Objective: The objective of the study was to evaluate the effects of maternal exposure to severe life events during different stage of gestation on preterm birth and infant birthweight. Study Design: A sample of 1800 women who delivered after 32 weeks' gestation were assessed with questionnaires that measured stressful life events during different stages of pregnancy. Demographic characteristics and birth outcomes were collected through the interviews and medical charts. Results: There was an increased risk of preterm birth among women with higher levels of life events stress during the first trimester (adjusted risk ratio, 2.40; 95% confidence interval, 1.13-5.09) and second trimester (adjusted risk ratio, 2.86; 95% confidence interval, 1.26-6.47). Each unit increase of perceived life events stress during first trimester was associated with a 99.09 g decrease in infant birthweight. Conclusion: Prenatal severe life events, especially in the first trimester, may play an important role in increasing the risk of preterm birth and low birthweight. © 2010 Mosby, Inc. All rights reserved.
Liao Y.,Xiamen University |
Zhou Y.,Maternal and Child Health Hospital |
Guo Q.,Maternal and Child Health Hospital |
Xie X.,Xiamen University |
And 3 more authors.
Journal of Clinical Microbiology | Year: 2013
Long-term infection with high-risk human papillomavirus (HPV) is the leading cause of cervical cancer, while infection with low-risk HPV is the major reason for condylomata acuminata. An accurate, rapid, and convenient assay that is able to simultaneously detect, genotype, and quantify HPV would be of great clinical value yet remains to be achieved. We developed a three-color real-time PCR assay that is able to analyze 30 predominant HPV types in three reactions. The amplification curves indicated the presence of HPV, melting curve analysis identified the HPV genotype, and the quantification cycle value determined the quantity. We applied this assay to 647 cervical swab samples, and the results were compared with those obtained with a commercial genotyping system. The proposed assay had a limit of detection of 5 to 50 copies per reaction and a dynamic range of 5×101 to 5×106 copies per reaction. A comparison study showed that the overall sample concordance with the comparison method was 91.6% and the type agreement was greater than 98.7%. The quantification study demonstrated that the loads of HPV type 16 in 30 samples with cervical intraepithelial neoplasia grade III (CIN III) lesions were significantly higher than those in samples with CIN I lesions or CIN II lesions, and the results were concordant with those of the comparison method. The increased information content, high throughput, and low cost would facilitate the use of this real-time PCR-based assay in a variety of clinical settings. Copyright © 2013, American Society for Microbiology.
Wu H.,Xiamen University |
Li Q.,Xiamen University |
Lu R.,Xiamen University |
Lu R.,Maternal and Child Health Hospital |
And 3 more authors.
Journal of Industrial Microbiology and Biotechnology | Year: 2010
The constant-rate fed-batch production of the polygalacturonic acid bioflocculant REA-11 was studied. A controlled sucrose-feeding strategy resulted in a slight improvement in biomass and a 7% reduction in flocculating activity compared with the batch process. When fed with a 3 g l-1 urea solution, the flocculating activity was enhanced to 720 U ml-1 in 36 h. High cell density (2.12 g l-1) and flocculating activity (820 U ml-1) were obtained in a 10-l fermentor by feeding with a sucrose-urea solution, with values of nearly two times and 50% higher than those of the batch process, respectively. Moreover, the residual sucrose declined to 2.4 g l-1, and residual urea decreased to 0.03 g l-1. Even higher flocculating activity of 920 U ml-1 and biomass of 3.26 g l-1 were obtained by feeding with a sucrose-urea solution in a pilot scale fermentation process, indicating the potential industrial utility of this constant-rate feeding strategy in bioflocculant production by Corynebacterium glutamicum. © 2010 Society for Industrial Microbiology.
Zhang Y.-P.,Maternal and Child Health Hospital |
Liu X.-H.,Maternal and Child Health Hospital |
Gao S.-H.,Maternal and Child Health Hospital |
Wang J.-M.,Maternal and Child Health Hospital |
And 4 more authors.
PLoS ONE | Year: 2012
Background: Preterm birth, the birth of an infant prior to 37 completed weeks of gestation, is the leading cause of perinatal morbidity and mortality. Preterm infants are at greater risk of respiratory, gastrointestinal and neurological diseases. Despite significant research in developed countries, little is known about the causes of preterm birth in many developing countries, especially China. This study investigates the association between sciodemographic data, obstetric risk factor, and preterm birth in five Maternal and Child Health hospitals in Beijing, China. Methods and Findings: A case-control study was conducted on 1391 women with preterm birth (case group) and 1391 women with term delivery (control group), who were interviewed within 48 hours of delivery. Sixteen potential factors were investigated and statistical analysis was performed by univariate analysis and logistic regression analysis. Univariate analysis showed that 14 of the 16 factors were associated with preterm birth. Inter-pregnancy interval and inherited diseases were not risk factors. Logistic regression analysis showed that obesity (odds ratio (OR) = 3.030, 95% confidence interval (CI) 1.166-7.869), stressful life events (OR = 5.535, 95%CI 2.315-13.231), sexual activity (OR = 1.674, 95%CI 1.279-2.191), placenta previa (OR 13.577, 95%CI 2.563-71.912), gestational diabetes mellitus (OR = 3.441, 95%CI1.694-6.991), hypertensive disorder complicating pregnancy (OR = 6.034, 95%CI = 3.401-10.704), history of preterm birth (OR = 20.888, 95%CI 2.519-173.218) and reproductive abnormalities (OR = 3.049, 95%CI 1.010-9.206) were independent risk factors. Women who lived in towns and cities (OR = 0.603, 95%CI 0.430-0.846), had a balanced diet (OR = 0.533, 95%CI 0.421-0.675) and had a record of prenatal care (OR = 0.261, 95%CI 0.134-0.510) were less likely to have preterm birth. Conclusions: Obesity, stressful life events, sexual activity, placenta previa, gestational diabetes mellitus, hypertensive disorder complicating pregnancy, history of preterm birth and reproductive abnormalities are independent risk factors to preterm birth. Identification of remedial factors may inform local health and education policy. © 2012 Zhang et al.