Wang X.,Chongqing Medical University |
Qin J.,Guiyang Maternal and Child Care Service Center |
Chen J.,Guiyang Maternal and Child Care Service Center |
Wang L.,Chongqing Medical University |
And 2 more authors.
International Journal of Hyperthermia | Year: 2013
Purpose: The aim of this study was to investigate the effect of high-intensity focused ultrasound (HIFU) on immune function in patients with uterine fibroids, in a randomised comparison to conventional myomectomy. Methods: The patients were assigned (1:1) to the HIFU group or the myomectomy (MY) group. Venous blood samples were collected 24h before and 24h and 72h after operation. The percentages of CD4+ and CD8+ T cells and natural killer (NK) cells were quantified by flow cytometry (FCM). Serum levels of interleukin-2 (IL-2), IL-6 and IL-10 were determined using enzyme-linked immunosorbent assay. Results: HIFU was associated with early ambulation, fewer post-operative complications, and shorter hospital stay (p<0.001). The percentages of CD4+ and CD8+ T cells and NK cells in the HIFU group were not significantly altered after treatment compared with before treatment. In contrast, the numbers of these cells in the MY group decreased significantly 24h after conventional myomectomy (p<0.001). The CD4 +/CD8+ T cell ratios were also decreased significantly 24h and 72h after conventional myomectomy (p<0.001). Serum levels of IL-6 and IL-10 increased after treatment in both groups. Peak IL-6 and IL-10 levels were significantly lower in the HIFU group than in the MY group (p<0.001). In contrast, IL-2 level decreased significantly in the MY group compared to the HIFU group at 24h post-operation (p<0.001). Conclusions: Short-term post-operative immune function is better preserved after HIFU treatment. Better preserved immune function may reflect a reduction in tissue trauma after HIFU treatment and contribute to reduced post-operative complications. © 2013 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.