Fatehgarh Chūriān, India
Fatehgarh Chūriān, India

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Gupta S.,Mata Gujri College | Prakash N.T.,Thapar University
Environmental Science and Pollution Research | Year: 2014

The present study was conducted to isolate and explore bacterial strains with a potential to sequester lead (Pb) and tolerate other heavy metals from industrial effluents and sediments. Out of the six bacterial strains isolated from seleniferous sites of Punjab, three isolates (RS-1, RS-2, and RS-3) were screened out for further growth-associated lead sequestration and molecular characterization on the basis of their tolerance toward lead and other heavy metals. Biomass and cell-free supernatant were analyzed for lead contents using ICP-MS after growth-associated lead sequestration studies in tryptone soya broth (pH = 7.2 ± 0.2) under aerobic conditions at 37 °C temperature. Almost 82 % and 70 % divalent lead was sequestered in cell pellets of RS-1 and RS-3, respectively while only 45 % of lead was found in cell pellet of RS-2 in the first 24 h. However, significant biosequestration of lead was observed in RS-2 after 48 h of incubation with concomitant increase in biomass. Simultaneously, morphological, biochemical, and physiological characterization of selected strains was carried out. 16S rRNA gene sequence of these isolates revealed their phylogenetic relationship with class Bacillaceae, a low G + C firmicutes showing 98 % homology with Bacillus sp. © 2014 Springer-Verlag Berlin Heidelberg.

Dhanjal N.I.K.,Mata Gujri College | Mittu B.,Punjabi University | Chauhan A.,National Institute of Technology Jalandhar | Gupta S.,Mata Gujri College
Journal of Environmental Science and Technology | Year: 2013

The textile industries produces considerably high amount of aquatic toxicity which is discharged directly into the environment before treated properly. The waste generation volume and load produced is hazardous in nature. Thus, this study explores the role of fungal biomass against pollution due to textiles dyes as degrading agent. This study will be beneficial for treating water effluent from textile industry and will decrease the pollution form environment with advanced technology for future use. In this study the evaluation of fungal species for the decolourization and degradation of textile dye has been carried. Four potential fungal strains (NS-1, NS-2, NS-9 and NS-10) were exploited after screening for the decolourization of Rubine Toner-12 dye under aerobic condition. Growth associated decolorization studies were carried out in Potato Dextrose Broth (PDB) supplemented with Rubine Toner-12. About 99% percent decolorization was achieved on supplementation with 10 mg L-1 of dye. Comparative spectrophotometric analysis of control and fungus inoculated medium supplemented with rubine toner-12 showed almost 100% decolorization in inoculated flasks. The fungus was identified to be Aspergillus niger. Maximum decolorization of Rubine Toner-12 was observed at pH 6. It is a better technique to check environmental pollution. © 2013 Asian Network for Scientific Information.

Chauhan S.,Himachal Pradesh University | Chaudhary P.,Himachal Pradesh University | Sharma K.,Himachal Pradesh University | Kumar K.,Himachal Pradesh University | Kiran,Mata Gujri College
Chemical Papers | Year: 2013

Volumetric and viscometric properties of glycine and methionine (amino acids) in a 0.2 vol. % amikacin sulphate (antibiotic drug) aqueous solution with the molality range of 0.025 mol kg-1-0.25 mol kg-1 were measured over the temperature range of 20 C-40 C at the interval of 5 C. Different parameters like apparent molar volume (V), apparent molar adiabatic compression (κ ), isentropic compression (κ S) along with other acoustical parameters were calculated. Parameters like viscous relaxation time (τ), free volume (V F), internal pressure (Π I), and molar cohesive energy (MCE) were calculated from dynamic viscosity measurements. The V values are positive in both cases, but with higher magnitude observed in methionine. These positive values of V are indicative of strong solute-solvent interactions at all temperatures. In case of methionine there is a sharp initial increase in the V values which become almost constant with further additions of the amino acid. Structural differences in the two amino acids studied are clearly reflected in the different nature of the plots of different parameters. In case of an amino acid-drug system, dynamic viscosity increase has been attributed to the increase in the hydrophilic-ionic and hydrophilic-hydrophilic interactions with the increase in the amino acid concentration which in turn may cause more frictional resistance to the flow of the solution. All other parameters are discussed in terms of solute-solvent and solvent-solvent interactions. © 2013 Institute of Chemistry, Slovak Academy of Sciences.

Singh A.,Mata Gujri College | Dhaliwal A.S.,Sant Longowal Institute of Engineering And Technology
MATEC Web of Conferences | Year: 2016

Total bremsstrahlung spectra in thick targets of Mo and Pt, produced by beta emitter 89Sr (end point energy = 1464 keV) have been studied in the photon energy range of 1-100 keV. The experimentally measured spectra measured were compared with the theoretical spectral distributions calculated from Elwert corrected (non relativistic) Bethe-Heitler [EBH] theory, modified Elwert factor (relativistic) Bethe-Heitler (Fmod BH) theory for ordinary bremsstrahlung (OB) and the modified Elwert factor (relativistic) Bethe-Heitler (Fmod BH+PB) theory, which includes the polarization bremsstrahlung (PB) into total bremsstrahlung (BS). The present results indicate the correctness of Fmod BH+PB theory in the low energy region, where the contributions of PB into BS are dominant. But at the middle and higher energy region of the bremsstrahlung spectrum, where the contribution of PB is negligible, the Fmod BH theory is more close to the experimental results. © Owned by the authors, published by EDP Sciences, 2016.

Kaur M.,Thapar University | Kaur V.,Mata Gujri College | Kumar S.,Thapar University
Physical Review C - Nuclear Physics | Year: 2013

The contribution of various components of potential towards balance energy is analyzed for the reactions of 2658Fe+2658Fe, 2858Ni+2858Ni, 3686Kr+4193Nb, and 79197Au+79197Au using an isospin-dependent quantum molecular dynamics (IQMD) model. We show that the balance energy changes with the addition of various components of potentials. Our calculations for the considered energy range clearly demonstrate the dominance of the Coulomb potential and momentum-dependent interactions over the other potentials for the lighter colliding systems. However, the contribution of the symmetry potential also becomes significant while handling heavier nuclei. © 2013 American Physical Society.

Gupta S.,Mata Gujri College | Nirwan J.,Mata Gujri College
International Journal of Environmental Science and Technology | Year: 2015

Heavy metal pollution affects environment adversely and leads to severe implications for both flora and fauna. In the present work, bacterial strain JS-1 was isolated with tolerance for different metals such as mercury (Hg), lead (Pb), cadmium (Cd), nickel (Ni), arsenic (As), tin (Sn), selenium (Se), zinc (Zn), chromium (Cr) and copper (Cu). JS-1 showed a significant tolerance for mercuric chloride (up to 5,000 μg/g) along with an efficient metal uptake and transformation. Growth of JS-1 was marginally affected on exposure to high mercury concentration due to acclimatization of the culture towards mercury. No mercury was found in cell-free supernatant after 96 h of incubation with 500 μg/g and 1,000 μg/g of mercury as an active ingredient. Almost all the mercury was found associated with cell biomass as determined by hydride generation atomic absorption spectroscopy. Only 60 % of mercury was sequestered in bacterial biomass on exposure to 2,000 and 5,000 μg/g mercury. As a detoxification mechanism, nearly 5 % of sequestered mercury was volatilized by the selected isolate (JS-1). Further X-ray diffraction analysis of deposited silvery grey biomass confirmed biotransformation of sequestered mercuric ions into monovalent mercury (Hg2Cl2), a non-bioavailable form of mercury. Culture was characterized morphologically, physiologically and biochemically. 16S rRNA gene sequence of JS-1 revealed its phylogenetic relationship and 98 % homology with Alcaligenes faecalis, a Gram-negative rod-shaped bacterium. © 2014, Islamic Azad University (IAU).

Gupta S.,Mata Gujri College | Bector S.,Mata Gujri College
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2013

Green chemistry is a boon for the development of safe, stable and ecofriendly nanostructures using biological tools. The present study was carried out to explore the potential of selected fungal strains for biosynthesis of intra- and extracellular gold nanostructures. Out of the seven cultures, two fungal strains (SBS-3 and SBS-7) were selected on the basis of development of dark pink colour in cell free supernatant and fungal beads, respectively indicative of extra- and intracellular gold nanoparticles production. Both biomass associated and cell free gold nanoparticles were characterized using X-ray diffractogram (XRD) analysis and transmission electron microscopy (TEM). XRD analysis confirmed crystalline, face-centered cubic lattice of metallic gold nanoparticles along with average crystallite size. A marginal difference in average crystallite size of extracellular (17.76 nm) and intracellular (26 and 22 nm) Au-nanostructures was observed using Scherrer equation. In TEM, a variety of shapes (triangles, spherical, hexagonal) were observed in both extra- and intracellular nanoparticles. 18S rRNA gene sequence analysis by multiple sequence alignment (BLAST) indicated 99 % homology of SBS-3 to Aspergillus fumigatus with 99 % alignment coverage and 98 % homology of SBS-7 to Aspergillus flavus with 98 % alignment coverage respectively. Native-PAGE and activity staining further confirmed enzyme linked synthesis of gold nanoparticles. © 2013 Springer Science+Business Media Dordrecht.

Bansal A.,Sant Longowal Institute of Engineering And Technology | Singh Sekhon J.,Akal Degree College | Singh Sekhon J.,Mata Gujri College | Verma S.S.,Sant Longowal Institute of Engineering And Technology
Journal of Modern Optics | Year: 2015

The individual noble metal nanoparticles (NPs) are combined to form alloys with improved optical response, cost effectiveness and better stability. The selection of noble metal alloy NPs for their better use in plasmonic applications is being made on the bases of surface plasmon resonance peak position, its intensity and full width at half maxima (FWHM). Presently, the effect of metal composition (x), aspect ratio (R), size and metal type on the longitudinal plasmon resonance (LPR) of noble metal Ag-Au alloy nanorods (NRs) has been studied by applying modified Gans theory including finite wavelength effects and found that the LPR shifts towards the longer wavelength region with increase in aspect ratio and size of the NR. Moreover, a linear relationship which is in good agreement to the experimental results between the plasmon resonance and aspect ratio has been obtained. The aspect ratio and NR width-dependent absorption efficiency and FWHM have also been calculated. Further, a negligible effect of metal composition and its type is found on the LPR. © 2015 Taylor & Francis.

Singh A.,Punjabi University | Dhau J.S.,University of Florida | Sharma N.,Punjabi University | Singh A.,Mata Gujri College
Inorganica Chimica Acta | Year: 2015

A convenient methodology which can be tailored to incorporate a chalcogen atom at the C-6 or C-3 position of 2-methoxypyridine (1) was developed. This was achieved by the regioselective lithiation of 1 in the presence and absence of BF3 metdot;Et2O. The reactions of the 2-methoxypyridine-BF3 adduct with lithium diisopropylamide (LDA) and subsequent trapping with chalcogen and iodomethane furnished a C-6 substituted product. However, the use of uncomplexed 1 led to the lithiation and chalcogenation at the C-3 position. The origin of selectivity in these reactions was studied by quantum chemical analysis. It was found that the coordination between the lithium (on C-6) and the fluoride of BF3 preponderates over the directed metalation ability of the methoxy unit in directing the lithiation process. © 2015 Elsevier B.V. All rights reserved..

Mittal P.,Baba Banda Singh Bahadur Engineering College | Singh N.,Mata Gujri College
Proceedings - 2016 2nd International Conference on Computational Intelligence and Communication Technology, CICT 2016 | Year: 2016

Speech provides a natural way of communication. Communicating with a device by talking to it provides a very comfortable interface, provided it understands your instructions well and performs action in time. But there are various challenges in doing so like memory, processing power, accuracy, timeliness, energy consumption, speaker dependency and many more. These challenges need to be considered before developing speech applications for mobile phones. PocketSphinx, Sphinx, Google API can be used for developing speech applications. Dragon Naturally speaking is a proprietary product which provides software for various speech applications. © 2016 IEEE.

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