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Columbia, MD, United States

Grant
Agency: Department of Health and Human Services | Branch: | Program: SBIR | Phase: Phase I | Award Amount: 164.92K | Year: 2014

DESCRIPTION (provided by applicant): Significant increase in the efficiency and speed of atmospheric pressure chemical ionization (APCI) in tandem mass spectrometry (MS/MS) will have a profound impact on its applications to the entire analytical field of characterization of drugs, metabolites, lipids, steroids, etc. A broad applicability of APCI-based MS analysis makes it a unique analytical tool specifically targeting trace amounts of many chemical and biological substances. We plan to build a novel APCI ion source based on atmospheric dielectric barrier discharge (DBD) to significantly improve efficiency and specificity of ACPI-MS based detection method. PUBLIC HEALTH RELEVANCE PUBLIC HEALTH RELEVANCE: Atmospheric pressure ionization is a broadly used technique in the analysis of complex chemical and biological samples providing reliable identification of drugs, disease-specific metabolites, steroids, antibiotics, etc. utilizing mass-spectrometers. We plan to develop a novel APCI source that


Grant
Agency: Department of Energy | Branch: | Program: SBIR | Phase: Phase I | Award Amount: 225.00K | Year: 2014

This project (technology transfer opportunity) is aimed at significant improving both the efficiency and performance of the ambient pressure nanoDESI ionization source for the comprehensive analysis of aerosol particles and surfaces including biological tissues by tandem mass spectrometry (MS/MS). Broad applicability of nanoDESI-based MS analysis makes it a unique analytical tool specifically targeting trace amounts of many chemical and biological substances. During implementation of this project we plan to develop and test a microfluidic chip and a novel subatmospheric ionization source which significantly improves efficiency and stability of the nanoDESI technique. The overall objective of this project is to build a robust commercial device facilitating mass analysis of aerosol and biological samples. The development of stable ionization and sample delivery techniques is the aim of Phase I. To facilitate operation of the device, the nanoDESI platform to be built will be made of two halves. One half will be integrated with an interface mounted on the inlet of mass spectrometer, while another half will be mounted on a XY stage holding the sample under analysis. Liquid connection between the halves will be provided by short piece of flexible capillary. Commercial applications include molecular-based aerosol analysis and analysis of biological tissues


Grant
Agency: Department of Health and Human Services | Branch: National Institutes of Health | Program: SBIR | Phase: Phase I | Award Amount: 144.31K | Year: 2016

DESCRIPTION provided by applicant Significant increase in the efficiency of electrospray ionization ESI and atmospheric pressure chemical ionization APCI sources in tandem mass spectrometry MS MS will have a profound impact on single cell MS based analysis and the entire analytical field of characterization of proteins lipids and metabolites Almost ionization efficiency of nanoflow ESI APCI sources makes it a unique analytical tool specifically applicable to the MS based analysis of zeptomole amounts of chemical and biological substances We plan to build a platform combining subatmospheric nanoflow ESI and APCI sources and very efficient ion collector to drastically improve sensitivity and speed of MS based identification of miniscule amount of biomaterials harvested from single cells PUBLIC HEALTH RELEVANCE A platform combining an ultralow flow liquid chromatography separation platform with nanoflow electrospray ionization source and high mass resolution mass spectrometer will be successfully used in the analysis of complex protein mixtures harvested from single cells The platform will provide reliable protein MS based identification from samples that contain zeptomole amounts of these proteins We plan to develop a novel sub atmospheric interface providing highly efficient collection of ions produced by sub atmospheric pressure ion sAPI sources that can be straightforwardly combined with gas phase separation methods This will drastically improve detection limits and accelerate characterization of proteins and metabolites from single cells using high resolution tandem mass spectrometry The platform can be easily incorporated into a design of a variety of commercial tandem mass spectrometers


Grant
Agency: Department of Health and Human Services | Branch: National Institutes of Health | Program: SBIR | Phase: Phase I | Award Amount: 150.00K | Year: 2016

DESCRIPTION provided by applicant Significant increase in the efficiency of matrix assisted laser desorption ionization MALDI method and utilization of lossless sub atmospheric pressure interface in mass spectrometry MS will have a profound impact on MS based tissue imaging and on the entire analytical field of characterization of proteins lipids and metabolites Proposed increase in ionization efficiency of MALDI sources will make it an indispensable tool for both research laboratories and in clinical settings We plan to build a platform combining atmospheric MALDI source and very efficient ion collector to drastically improve sensitivity and speed of MS based analysis of miniscule amount of biomaterials harvested from tissues PUBLIC HEALTH RELEVANCE A mass spectrometry MS imaging platform utilizing an atmospheric pressure matrix assisted ionization source and ion funnel will be hyphenated with high mass resolution mass spectrometer for the analysis of biological molecules from tissue samples The platform will significantly improve the method sensitivity compared to that achieved using commercial ion sources We plan to develop a novel interface providing highly efficient ionization of analytes harvested from tissue samples and lossless collection of produced ions in the low pressure ion funnel The interface can be straightforwardly combined with gas phase separation methods A use of novel interface will drastically improve detection limits of the MS imaging technology leading to better characterization of protein and metabolite distribution in tissue samples The platform can be easily incorporated into a variety of commercial tandem mass spectrometers


Grant
Agency: Department of Health and Human Services | Branch: | Program: SBIR | Phase: Phase I | Award Amount: 166.87K | Year: 2012

DESCRIPTION (provided by applicant): Increasing efficiency of ion detection in Fourier Transform Mass Spectrometry (FTMS) can have a tremendous impact on its applications in proteomics and entire analytical field. At present, the sensitivity of FTMS instruments is limited by electrical noise of semiconductor circuits utilized to amplify signals from electrodes of Ion Cyclotron Resonance cells. By using superconductor devices based on quantization of magnetic flux instead of semiconductor circuits we will improve sensitivity of the ion detection in FTMS by a factor of 10 or more. To reach high dynamic range in our desktop FTMS system, we also plan to design and test a new Ion Cyclotron Resonance cell, which will allow us to attain high mass resolution and mass accuracy with fewer analyzed ions. PUBLIC HEALTH RELEVANCE: Fourier Transform (FT) Mass Spectrometers (MS) are broadly used in proteomic and metabolomic studies of complex biological samples. These mass spectrometers demonstrate record-high mass accuracy and mass resolution that are vital to achieve high reliability in comparative analyses of normal vs disease proteomes. We plan to develop a novel detection system that will improve sensitivity and dynamic range of the FTMS systems by a factor of 10or more, which will allow much better characterization of disease-specific biomarkers in the FTMS-based analysis of biological fluids or tissues.

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