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Bakhtiyari S.,Ilam University | Haghani K.,Tarbiat Modares University | Farhadi E.,Iranian Blood Transfusion Organization IBTO | Soukhtanloo M.,Mashhad Medical University | And 2 more authors.
Hybridoma | Year: 2010

Mycobacterium contains several immunologically active substances, which play a principal role in mycobacterial diseases. The majority of the highly antigenic proteins present in mycobacterial homogenates are components of the A60 complex. In this study, A60 antigen was prepared from cytoplasm of Mycobacterium bovis Bacillus Calmette-Guerin (BCG). Cytoplasm was fractionated by passage through the column of sepharose 6B and ConA-sepharose 4B. After purification of spleen cells of the immunized mice, the cells were fused with SP2/0 myeloma cells. Four clone cell lines producing antibody against A60 antigens were established and each clone was tested for immunoreactivity against purified A60 by ELISA and immunoblotting. The clone designated DEB7 reacted strongly with A60. Immunoblotting using MAb DEB7 showed that this MAb binds to a single protein of A60 subunit with a molecular weight of 65kDa. This subunit of A60 M. bovis recognized by DEB7 MAb could be used to increase the sensitivity and specificity of immunoassay or other potential roles in mycobacterium infection. © Copyright 2010, Mary Ann Liebert, Inc..

Vahabi S.,Shahid Beheshti University | Amirizadeh N.,Iranian Blood Transfusion Organization | Shokrgozar M.A.,Pasteur Institute of Iran | Mofeed R.,Shahid Beheshti University | And 4 more authors.
Chang Gung Medical Journal | Year: 2012

Background: Recently, tissue engineering has been introduced as a regenerative treatment for bone defects. There is some evidence showing bone regeneration from mesenchymal stem cells (MSC) loaded on hydroxyapatite β-tricalcium phosphate (HA/TCP) as a scaffold in large defects. This study aimed to compare the quality and quantity of regenerated bone using Bio-Oss, HA/TCP and MSC loaded HA/TCP scaffolds. Methods: Mesenchymal stem cells were aspirated from iliac crest bone marrow after extracting the first, second and third premolars and the first molar in five mature hybrid dogs. The cells were cultured and their osteogenic differentiation potential was evaluated after the third cell passage using Alizarin red staining in experimental conditions. The HA/TCP scaffold (3 x 3 x 3 mm) was loaded with undifferentiated mesenchymal stem cells. Bilateral bone defects were then prepared in the jaws using trephine burs. The defects were randomly filled with HA/TCP, Bio-Oss, or HA/TCP + MSCs. One defect served as a control and was left as an empty cavity. All defects except the control defect were covered with an absorbable membrane. Histological and histomorphometric evaluations were conducted after 6 weeks and data were subjected to analysis of variance (ANOVA) (p < 0.05). Results: The empty cavity demonstrated more bone formation (60.80%) than the HA/TCP (44.93%) and Bio-Oss (40.60%) (p < 0.05) groups. However, the difference from the HA/TCP + MSCs group was not significant (46.38%) (p > 0.05). Conclusion: An MSC-loaded HA/TCP scaffold is a more effective alternative than Bio- OSS or HA/TCP in inducing bone regeneration.

Taghavi S.M.,Mashhad Medical University | Fatemi S.S.,Mashhad Medical University | Rokni H.,Mashhad Medical University
Medical Journal of Malaysia | Year: 2012

Ergot-derived dopamine D2 receptor agonists are the usual treatment of hyperprolactinemia and Parkinson's disease and recently bromocriptine has been approved for the treatment of type 2 diabetes. The aim of this study was the evaluation of short-term effect of cabergoline in poorly controlled diabetic patients with oral agent failure who refused insulin therapy. Methods: This study was performed in 17 overweight women and men with type 2 diabetes with persistent hyperglycemia in spite of treatment with maximum dose of sulfonylurea, metformin and pioglitazone. 10 patients (group I) randomized to be treated with cabergoline 0.5 mg weekly for 3 months and 7 patients (group II) with placebo. Fasting and postprandial plasma glucose concentration and HbA1c measured in beginning and end of the study. Results: FBS decreased from 210.70± 21.29 to 144.90± 26.56 mg/dl in cabergoline group whereas it decreased in placebo group insignificantly. Postprandial blood glucose decreased from 264.2±28 mg/dl to 203.6±34.34 mg/dl in cabergoline group whereas it increased in placebo group insignificantly.HbA1c decreased in cabergoline group from 8.48±0.44 to 7.7±0.11 whereas in control group it increased insignificantly from 8.7±0.33 to 8.8±0.16. Conclusion: Cabergoline improves glycemic control in type 2 diabetic patients with oral agent failure. It reduces both fasting and postprandial plasma glucose levels and causes 0.45-1.11 reduction in HbA1c.

Morteza Taghavi S.,Mashhad Medical University | Rokni H.,Mashhad Medical University | Fatemi S.,Mashhad Medical University
Diabetes and Vascular Disease Research | Year: 2011

Objective: To assess the effect of metformin administration on thyroid function in overweight women with polycystic ovarian syndrome (PCOS). Methods: Twenty-seven overweight women with PCOS and hypothyroidism were selected. Fifteen patients (group I) were treated with metformin 1500 mg/day for 6 months and 12 patients (group II) with placebo. Serum thyrotropin (TSH), free T3 and free T4 were measured at baseline and 6 months after the beginning of the study. Results: A significant decrease (p<0.001) in TSH levels was observed in group I but not in group II subjects after 6 months of metformin treatment. No significant change in free T3 and free T4 was observed throughout the study in any group. Conclusion: In obese PCOS patients with primary hypothyroidism, metformin results in a significant fall and sometimes normalisation of TSH, without causing any reciprocal changes in other thyroid function parameters. © The Author(s) 2011.

Morteza T.,Mashhad Medical University | Sedigheh F.,Mashhad Medical University | Reza F.H.,Mashhad Medical University | Reza K.A.,Mashhad Medical University
Diabetologia Croatica | Year: 2010

The possible role of some viral infections in the pathogenesis of type 1 diabetes (T1DM) has been postulated. There is increasing evidence for the viral etiology of type 2 diabetes mellitus (T2DM). Human T cell leukemia virus type 1 (HTLV-I) is endemic in some areas of the world. It is also endemic in Mashhad, northeast Iran. The objective of the present study was to estimate the prevalence of glucose abnormalities in HTLV-I carriers in Mashhad. A total of 177 HTLV-I carriers older than 20 years were compared with 220 sex-, age- and BMI-matched controls without the infection. Blood samples were collected in fasting state and assayed for serum glucose. We also compared the results on the prevalence of glucose abnormalities in the general population of Mashhad, obtained in a large study. The prevalence of glucose abnormalities was significantly higher in HTLV-I carriers than in controls (10.16% vs. 5% for diabetes and 25.98% vs. 6.36% for impaired fasting glucose, P<0.05). The prevalence of glucose abnormalities was also significantly higher from the prevalence recorded in a large epidemiologic study performed in Mashhad. In conclusion, the prevalence of glucose abnormalities was significantly higher in HTLV-I carriers than in controls. HTLV-I infection may increase the risk of diabetes.

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