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Zagreb, Croatia

Antonovic I.,Slavka Kolara 45 | Brigic A.,Rooseveltov trg | Bedek J.,Croatian Biospeleological Society | Sedlar Z.,Marulicev trg
Natura Croatica

This paper presents the distribution of the terrestrial isopod Hyloniscus adonis (Verhoeff, 1927) in Croatia. It was recorded for the first time at the edge of Dubravica peat bog and the adjacent forest. Additionally, it was collected at the edges of Đon močvar peat bog. Revision of the terrestrial isopod collection of the Croatian Biospeleological Society revealed that this species also occurs in central and eastern part of Croatia. Our results reveal that H. adonis is a hygrophilic species inhabiting various types of forests in the colline and montane belts. It prefers humid soils with thick litter and humus layers and most probably seldom leaves its shelter. H. adonis is extremely rare in open bog habitats, most likely due to the harsh environmental conditions (e.g. high soil humidity, low pH values) and can be considered as a tyrphoxenous species. Additionally, it was also found in the entrance zones of caves, most likely due to the favourable microclimatic conditions. It seems to be a temporary cave dweller and can be classified as a trogloxenous species. © 2015, Croatian Natural History Museum. All rights reserved. Source

Bielen A.,University of Zagreb | Vladusic T.,University of Zagreb | Kuharic N.,Rooseveltov trg | Hudina S.,Rooseveltov trg | And 3 more authors.
Periodicum Biologorum

Background and Purpose: Te signal crayfsh Pacifastacus leniusculus (Dana, 1852) is one of the most successful invasive species of crayfsh in European freshwaters, an extremely diverse though endangered group of ecosystems. Te main goal of this study was to functionally characterize multixenobiotic resistance (MXR) mechanism defense activity in P. lenius-culus tissues for the frst time. MXR mechanism protects the cell from a wide variety of toxic compounds, and it is mediated by the transport activity of ATP-binding cassette (ABC) proteins. Materials and Methods: MXR transporter activity dye assay was performed by using fuorescent model substrate rhodamine B (RB) in combination with inhibitors of MXR efux pumps: MK571 and Verapamil, known to inhibit multidrug resistance-associated proteins (MRP) and P-glycoprotein (P-gp), respectively. In this assay, the increase in intracellular fuorescence of the substrate dye, indicates inhibition of MXR efux protein pumps. Te assay was performed in three diferent tissues (gills, hepatopancreas, tail muscle). Additionally, tissues were exposed to selected heavy metals – mercury (HgCl2) and zinc (ZnCl2), known to occur in open freshwaters as pollutants. Results: Optimal time for RB accumulation in gills and hepatopancreas was determined to be 30 minutes. RB efux in gills was inhibited by MK571 and in hepatopancreas by Verapamil, suggesting that multidrug resistance-associated proteins are dominant in gills of P. leniusculus, and P-glycoprotein in hepatopancreas. Finally, inhibitory efect of mercury (HgCl2: 10 and 20 µM) and zinc (ZnCl2: 5–20 µM) on multixenobiotic resistance mechanism activity in gills, and only mercury in hepatopancreas, was detected. Conclusions: Te results for the frst time demonstrate the presence of multixenobiotic resistance mechanism efux activity as an important tissue specifc defense mechanism in P. leniusculus and provide the basis for future molecular and toxicological studies of this invasive and adaptable species. © 2014, Croatian Society of Natural Sciences. All rights Reserved. Source

Pecek G.,Croatian Center for Agriculture | Pavlovic D.M.,Marulicev trg | Babic S.,Marulicev trg
International Journal of Environmental Analytical Chemistry

A method for analysis of 20 commonly used pesticides in surface water based on solid-phase extraction and gas chromatography-mass spectrometry was proposed. During method development the key parameters that can affect SPE extraction and determination such as selection of efficient SPE sorbent, pH of water sample, type and volume of elution solvent, breakthrough volume and matrix effects were investigated. The method was validated using spring water spiked with appropriate concentration of pesticides. The obtained correlation coefficients were in range 0.9972-1.000, limits of detection (LOD) were 0.001-0.5 μg L-1 and the limits of quantification (LOQ) were 0.005-1 μg L-1 depending on a pesticide. Much higher LOD (20 μg L-1) and LOQ (50 μg L-1) values were obtained for bentazone. The influence of matrix was assessed using real water samples spiked with appropriate concentration of pesticide standards solution. Both signal enhancement and suppression were observed, depending on a pesticide, therefore standard addition method was used for pesticides determination. The developed method was applied on real water samples taken in close vicinity of agricultural fields. Many of the targeted pesticides were found in the samples and the results are presented in this article. © 2013 Copyright Taylor and Francis Group, LLC. Source

Dolar D.,Marulicev trg | Perisa M.,Marulicev trg | Kosutic K.,Marulicev trg | Babic S.,Marulicev trg
Desalination and Water Treatment

Once released into the environment pharmaceuticals undergo a series of processes leading to their degradation. Photolysis induced by sunlight is the most important way of pharmaceuticals degradation in water. In this work the removal of enrofloxacin (ENRO) and its photodegradation products with nanofiltration (NF) and reverse osmosis (RO) membranes was investigated. ENRO solution (10mgL-1, pH= 8) was irradiated under artificial light for 2 h. As a result the degradation of ENRO produced three photoproducts with molecular weights (MW) 389, 373 and 114 gmol-1. Reverse osmosis (LFC-1, SWC4+) and tight nanofiltration (NF90) membranes almost removed (>99.99%) all the compounds (parent and photodegradation products), but the other nanofiltration (DK, NF and HL) membranes removed more than 92%, with one exception (36.96% for the compound with MW 114 gmol-1) obtained using HL membrane. © 2013 Desalination Publications. Source

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