Nagano-shi, Japan
Nagano-shi, Japan

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PubMed | Marukome Company, Kyoto University and Nara Women's University
Type: Journal Article | Journal: Journal of agricultural and food chemistry | Year: 2016

Koji is made from grains fermented with Aspergillus oryzae and is essential for the production of many traditional Japanese foods. Many previous studies have shown that koji contributes to the improvement of dyslipidemia. However, little is known regarding the underlying mechanism of this effect. Furthermore, the compound contributing to the activation of lipid metabolism is unknown. We demonstrated that rice koji extract (RKE) induces the mRNA expression of peroxisome proliferator-activated receptor alpha (PPAR) target genes, which promotes lipid metabolism in murine hepatocytes. This effect was not observed in PPAR-KO hepatocytes. We also demonstrated that RKE contained linolenic acid (LIA), oleic acid (OA), and hydroxyoctadecadienoic acids (HODEs), which activate PPAR, using LC-MS analysis. Our findings suggest that RKE, containing LIA, OA, and HODEs, could be valuable in improving dyslipidemia via PPAR activation.


Ohata E.,Shinshu University | Yoshida S.,Shinshu University | Masuda T.,Shinshu University | Kitagawa M.,Marukome Co. | And 3 more authors.
Food Science and Technology Research | Year: 2011

We measured the ability of 100 strains of lactic acid bacteria (LAB) isolated from miso to induce Th1-type cytokines (interleukin (IL)-12 and IFN-γ) using mouse Peyer's patch (PP) cells, and selected Tetragenococcus halophilus MN45 strain (MN45) as the strain with the potential for a strong anti-allergic effect. MN45 cultured in medium containing 15% NaCl for more than 2 days showed the strongest activity of Th1-type cytokine induction among MN45 cultured at various NaCl concentrations and culture times. Next, we analyzed the ability of MN45 to suppress IgE production and the mechanism thereof by neutralizing IL-12 and IFN-γ using PP cells and splenocytes from ovalbumin (OVA)-induced allergic diarrhea model mice. Analysis revealed that MN45 induced IL-12 first, and then IL-12 induced IFN-γ strongly and inhibited IgE production. These results indicate that MN45 may exert an anti-allergic effect.


Ohata E.,Shinshu University | Yoshida S.,Shinshu University | Masuda T.,Shinshu University | Kitagawa M.,Marukome Co. | And 3 more authors.
Food Science and Technology Research | Year: 2011

We investigated the inhibitory effects of oral administration of Tetragenococcus halophilus MN45 (MN45) isolated from miso on the development of atopic dermatitis (AD) using NC/Nga AD model mice. NC/Nga mice were fed a diet containing 0.05% or 0.5% MN45 (0.05% or 0.5% MN45 groups) or lacking MN45 (control group). Mice were sensitized and boosted with picryl chloride by topical application once per week. IgE production in serum, clinical score and ear thickness in both MN45 groups were significantly suppressed. In addition, IgE and IL-17 production from splenocytes from mice in both MN45 groups were significantly decreased. IL-4 production from splenocytes in the 0.5% MN45 group decreased significantly, while IL-10 production from splenocytes in the 0.05% MN45 group increased significantly. These results demonstrate that intake of MN45 is effective in preventing and alleviating the development of type-1 allergic symptoms in humans.

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