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Nagao T.,Osaka Municipal Technical Research Institute | Watanabe Y.,Osaka Municipal Technical Research Institute | Maruyama K.,Maruha Nichiro Holdings Inc. | Momokawa Y.,Kinki University | And 2 more authors.
New Biotechnology | Year: 2011

Docosahexaenoic acid (DHA)-rich oil has been industrially produced by selective hydrolysis of tuna oil with a lipase that acts weakly on DHA. The free fatty acids (FFAs) generated in this process as by-products contain a high DHA concentration (46. wt%) but are treated as industrial waste. This study attempted to reuse these by-product FFAs using a one-pot process, and succeeded in producing triacylglycerols (TAGs) through the esterification of the by-product FFAs with glycerol using immobilized Rhizomucor miehei lipase. Regiospecific analysis of the resulting TAGs showed that the content of DHA at the sn-1(3) position (51.7. mol%) was higher than the content of DHA at the sn-2 position (17.3. mol%). The DHA distribution in TAGs synthesized in this study was similar to the DHA distribution in TAGs from seal oil. © 2010 Elsevier B.V. Source

Fukushima T.,Fukuoka Dental College | Ohno J.,Fukuoka Dental College | Imayoshi R.,Fukuoka Dental College | Mori N.,Fukuoka Dental College | And 3 more authors.
Journal of Materials Science: Materials in Medicine | Year: 2011

A DNA/protamine complex powder was prepared by reaction between DNA and protamine sulfate solution with stirring in order to develop a new injectable biomaterials for dental therapy. The powder of DNA/protamine complex became paste by kneading the complex powder and distilled water. Complex formation was confirmed by FT-IR measurement. The complex paste had a porous structure and its viscosity was approximately 280.1 Pas. The paste could easily pass through a needle of 0.25 mm internal diameter. It seemed that DNA/protamine complex paste has suitable viscosity for clinical use as an injectable biomaterial. Although, the complex paste delayed the growth speed of Staphylococcus aureus, Pseudomonas aeruginosa, Porphyromonas gingivalis and Prevotella intermedia for limited periods, it cannot kill and inhibit growing bacteria. The complex paste disk showed a mild tissue response and gradually degraded after the implantation into the soft tissue of rats. These results suggested that this DNA/protamine complex paste could be a useful material for a biodegradable biomaterial. In particular, this paste will be applicable as an injectable biomaterial using syringe for the repair of defects of living tissue, GBR treatment and/or GTR treatment in dentistry. © 2011 Springer Science+Business Media, LLC. Source

Zhou L.,Nihon University | Zhou L.,Anhui Medical University | Matsumura H.,Nihon University | Mezawa M.,Nihon University | And 4 more authors.
Gene | Year: 2013

Protamine is a small, arginine-rich, nuclear protein that replaces histone late in the haploid phase of spermatogenesis and is believed to be essential for sperm head condensation and DNA stabilization. Protamine has many biological activities and has roles in hematopoiesis, immune responses, the nervous system and bone metabolism. Bone sialoprotein (BSP) is a mineralized connective tissue-specific protein expressed in differentiated osteoblasts that appears to function in the initial mineralization of bone. Protamine (71.35. ng/ml) increased BSP mRNA levels by 6. h in osteoblast-like ROS 17/2.8 cells. In a transient transfection assay, protamine (71.35. ng/ml) increased luciferase activity of the construct (- 116 to +. 60) in ROS 17/2.8 cells and rat bone marrow stromal cells. Luciferase activities induced by protamine were blocked by protein kinase A, tyrosine kinase and ERK1/2 inhibitors. Introduction of 2. bp mutations to the luciferase constructs showed that the effects of protamine were mediated by a cAMP response element (CRE), a fibroblast growth factor 2 response element (FRE) and a homeodomain protein-binding site (HOX). Gel shift analyses showed that protamine (71.35. ng/ml) increased the nuclear protein binding to CRE, FRE and HOX. CREB, phospho-CREB, c-Fos, c-Jun, JunD and Fra2 antibodies disrupted the formation of CRE-protein complexes. Dlx5, Msx2, Runx2 and Smad1 antibodies disrupted FRE- and HOX-protein complex formations. These studies demonstrate that protamine induces BSP transcription by targeting CRE, FRE and HOX sites in the proximal promoter of the rat BSP gene. Moreover, phospho-CREB, c-Fos, c-Jun, JunD, Fra2, Dlx5, Msx2, Runx2 and Smadl transcription factors appear to be key regulators of protamine effects on BSP transcription. © 2012 Elsevier B.V. Source

Maruha Nichiro Holdings Inc., INC., MARUHA NICHIRO FOODS, Nichiro Corporation and Maruha Corporation | Date: 2011-08-16

Cosmetics. Pharmaceutical preparations, namely, anticoagulant preparations, antidepressant preparations, chondriotin preparations, and pharmaceutical preparations for inhalation for the treatment of pulmonary hypertension. Edible fish; edible crustaceans, namely, shrimp, lobster, crab; frozen vegetables and fruits; processed meat products, namely, canned cooked meats, bottled cooked meats, prepackaged meats, and butchered raw meat; processed fish products, namely, canned fish, fish fillets, flakes of dried fish; processed vegetables and fruits, laver processed in dried, toasted and flaked form and for sprinkling on rice in hot water. Bread; confectionery, namely, pastilles, fondants, fruit jellies, instant sherbet mixes, instant ice cream mixes, and frozen confectionery; and seasonings.

Maruha Nichiro Holdings Inc. | Date: 2012-02-29

This invention provides a method for producing ethanol from mannitol using yeast and a yeast strain that produces ethanol from mannitol. The method for producing ethanol from mannitol comprises culturing yeast strains capable of mannitol assimilation and ethanol production from mannitol in a medium containing mannitol.

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